Micro OS-ELISA: Rapid noncompetitive detection of a small biomarkerpeptide by open-sandwich enzyme-linked immunosorbent assay (OS-ELISA) integrated into microfluidic device

Lab on a Chip ◽  
2010 ◽  
Vol 10 (1) ◽  
pp. 92-100 ◽  
Author(s):  
Masaki Ihara ◽  
Amane Yoshikawa ◽  
Yushu Wu ◽  
Hiroko Takahashi ◽  
Kazuma Mawatari ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

A lab in a bento box: an autonomous centrifugal microfluidic system for an enzyme-linked immunosorbent assay

2020 ◽  
Vol 12 (40) ◽  
pp. 4858-4866
Author(s):  
Takaaki Abe ◽  
Shunya Okamoto ◽  
Akinobu Taniguchi ◽  
Michiyasu Fukui ◽  
Akinobu Yamaguchi ◽  
...  
Keyword(s):  
Injection Molding ◽  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Device Driver ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay

In this paper, we report on the demonstration of a portable immunoassay system consisting of a small centrifugal microfluidic device driver (bento box) and a centrifugal microfluidic device made of polypropylene and fabricated by injection molding.

Direct digital manufacturing of a mini-centrifuge-driven centrifugal microfluidic device and demonstration of a smartphone-based colorimetric enzyme-linked immunosorbent assay

2016 ◽  
Vol 8 (2) ◽  
pp. 256-262 ◽  
Author(s):  
Yoshiaki Ukita ◽  
Yuichi Utsumi ◽  
Yuzuru Takamura
Keyword(s):  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Three Dimensional ◽  
Enzyme Linked Immunosorbent Assay ◽  
Digital Manufacturing ◽  
3D Printer ◽  
Direct Digital Manufacturing

This study reports the first demonstration of an enzyme-linked immunosorbent assay (ELISA) using a microfluidic device that was fabricated in a three-dimensional (3D) printer.

Surface Modification for Enhancing Antibody Binding on Polymer-Based Microfluidic Device for Enzyme-Linked Immunosorbent Assay

Langmuir ◽  
2006 ◽  
Vol 22 (22) ◽  
pp. 9458-9467 ◽  
Author(s):  
Yunling Bai ◽  
Chee Guan Koh ◽  
Megan Boreman ◽  
Yi-Je Juang ◽  
I-Ching Tang ◽  
...  
Keyword(s):  
Surface Modification ◽  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Antibody Binding ◽  
Enzyme Linked Immunosorbent Assay

Plasminogen Interactions with Immobilized Fibrinogen

1989 ◽  
Vol 62 (04) ◽  
pp. 1078-1082 ◽  
Author(s):  
Burt Adelman ◽  
Patricia Ouynn
Keyword(s):  
Immunosorbent Assay ◽  
Aminocaproic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Regions ◽  
Dose Dependent Fashion ◽  
Epsilon Aminocaproic Acid ◽  
Dose Dependent

SummaryThis report describes the binding of plasminogen to fibrinogen adsorbed onto polystyrene wells. Binding was determined by enzyme linked immunosorbent assay. Both glu- and lys-plasminogen bound to immobilized fibrinogen in a dose-dependent fashion. However, more lys- than glu-plasminogen bound when equal concentrations of either were added to immobilized fibrinogen. Plasminogen binding was inhibited by epsilon aminocaproic acid indicating that binding was mediated via lysine-binding regions of plasminogen. Soluble fibrinogen added in excess of immobilized fibrinogen did not compete for plasminogen binding but fibrinogen fragments produced by plasmin digestion of fibrinogen did. Treatment of immobilized fibrinogen with thrombin caused a small but significant (p <0.01) increase in plasminogen binding. These studies demonstrate that immobilized fibrinogen binds both glu- and lys-plasminogen and that binding is mediated via lysine-binding regions. These interactions may facilitate plasminogen binding to fibrinogen adsorbed on to surfaces and to cells such as platelets which bind fibrinogen.

Sign in / Sign up

Export Citation Format

Share Document