Surface Modification for Enhancing Antibody Binding on Polymer-Based Microfluidic Device for Enzyme-Linked Immunosorbent Assay

Langmuir ◽  
2006 ◽  
Vol 22 (22) ◽  
pp. 9458-9467 ◽  
Author(s):  
Yunling Bai ◽  
Chee Guan Koh ◽  
Megan Boreman ◽  
Yi-Je Juang ◽  
I-Ching Tang ◽  
...  
Keyword(s):  
Surface Modification ◽  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Antibody Binding ◽  
Enzyme Linked Immunosorbent Assay

A lab in a bento box: an autonomous centrifugal microfluidic system for an enzyme-linked immunosorbent assay

2020 ◽  
Vol 12 (40) ◽  
pp. 4858-4866
Author(s):  
Takaaki Abe ◽  
Shunya Okamoto ◽  
Akinobu Taniguchi ◽  
Michiyasu Fukui ◽  
Akinobu Yamaguchi ◽  
...  
Keyword(s):  
Injection Molding ◽  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Device Driver ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay

In this paper, we report on the demonstration of a portable immunoassay system consisting of a small centrifugal microfluidic device driver (bento box) and a centrifugal microfluidic device made of polypropylene and fabricated by injection molding.

scholarly journals Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline

2021 ◽  
Vol 5 (5) ◽  
pp. 447-453
Author(s):  
Rachmat Hidayat ◽  
Patricia Wulandari
Keyword(s):  
Immunosorbent Assay ◽  
Solid Surface ◽  
Color Change ◽  
Antibody Binding ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hormone Levels ◽  
Elisa Technique ◽  
Antibody Complex ◽  
Antigen Antibody ◽  
Detection Signal

ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding. In the ELISA technique, antigen immobilization will be carried out on a solid surface, then bound with antibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the form of a color change will be formed due to the reaction between the enzyme and the substrate.

scholarly journals The landscape of antibody binding in SARS-CoV-2 infection

2020 ◽  
Author(s):  
Anna S Heffron ◽  
Sean J McIlwain ◽  
Maya F Amjadi ◽  
David A Baker ◽  
Saniya Khullar ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Diagnostic Accuracy ◽  
B Cell ◽  
Immunosorbent Assay ◽  
Antibody Binding ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Peptide Microarray ◽  
B Cell Epitopes ◽  
Homologous Peptide

The search for potential antibody-based diagnostics, vaccines, and therapeutics for pandemic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has focused almost exclusively on the spike (S) and nucleocapsid (N) proteins. Coronavirus membrane (M), ORF3a, and ORF8 proteins are humoral immunogens in other coronaviruses (CoVs) but remain largely uninvestigated for SARS-CoV-2. Here we use ultradense peptide microarray mapping to show that SARS-CoV-2 infection induces robust antibody responses to epitopes throughout the SARS-CoV-2 proteome, particularly in M, in which one epitope achieved excellent diagnostic accuracy. We map 79 B cell epitopes throughout the SARS-CoV-2 proteome and demonstrate that antibodies that develop in response to SARS-CoV-2 infection bind homologous peptide sequences in the six other known human CoVs. We also confirm reactivity against four of our top-ranking epitopes by enzyme-linked immunosorbent assay (ELISA). Illness severity correlated with increased reactivity to nine SARS-CoV-2 epitopes in S, M, N, and ORF3a in our population. Our results demonstrate previously unknown, highly reactive B cell epitopes throughout the full proteome of SARS-CoV-2 and other CoV proteins.

Micro OS-ELISA: Rapid noncompetitive detection of a small biomarkerpeptide by open-sandwich enzyme-linked immunosorbent assay (OS-ELISA) integrated into microfluidic device

Lab on a Chip ◽  
2010 ◽  
Vol 10 (1) ◽  
pp. 92-100 ◽  
Author(s):  
Masaki Ihara ◽  
Amane Yoshikawa ◽  
Yushu Wu ◽  
Hiroko Takahashi ◽  
Kazuma Mawatari ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

Direct digital manufacturing of a mini-centrifuge-driven centrifugal microfluidic device and demonstration of a smartphone-based colorimetric enzyme-linked immunosorbent assay

2016 ◽  
Vol 8 (2) ◽  
pp. 256-262 ◽  
Author(s):  
Yoshiaki Ukita ◽  
Yuichi Utsumi ◽  
Yuzuru Takamura
Keyword(s):  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Three Dimensional ◽  
Enzyme Linked Immunosorbent Assay ◽  
Digital Manufacturing ◽  
3D Printer ◽  
Direct Digital Manufacturing

This study reports the first demonstration of an enzyme-linked immunosorbent assay (ELISA) using a microfluidic device that was fabricated in a three-dimensional (3D) printer.

scholarly journals Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline

2021 ◽  
Vol 5 (2) ◽  
pp. 352-358
Author(s):  
Rachmat Hidayat ◽  
Patricia Wulandari
Keyword(s):  
Immunosorbent Assay ◽  
Solid Surface ◽  
Color Change ◽  
Antibody Binding ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hormone Levels ◽  
Elisa Technique ◽  
Antibody Complex ◽  
Antigen Antibody ◽  
Detection Signal

A B S T R A C TELISA (Enzyme-linked immunosorbent assay) is a technique used to assessthe quantification of peptide, protein, antibody and hormone levels, basedon the principle of antigen-antibody binding. In the ELISA technique, antigenimmobilization will be carried out on a solid surface, then bound withantibodies to form an antigen-antibody bond complex, where the antigen-antibody complex is bound to the enzyme. The detection signal in the formof a color change will be formed due to the reaction between the enzyme andthe substrate.

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