Droplet-based blood group antibody screening with laser incubation

The Analyst ◽  
2021 ◽  
Vol 146 (8) ◽  
pp. 2499-2505
Author(s):  
Clare A. Manderson ◽  
Heather McLiesh ◽  
Rico F. Tabor ◽  
Gil Garnier
Keyword(s):  
Blood Group ◽  
Hydrophobic Surface ◽  
Paper Strip ◽  
Antibody Screening ◽  
Indirect Antiglobulin Test ◽  
Antiglobulin Test

Complete indirect antiglobulin test (IAT) in a single diagnostic incorporating a laser-incubated droplet on a hydrophobic surface with read-out provided by a paper strip.

scholarly journals Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Dietmar Enko ◽  
Claudia Habres ◽  
Franz Wallner ◽  
Barbara Mayr ◽  
Gabriele Halwachs-Baumann
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Samples ◽  
Antibody Screening ◽  
Indirect Antiglobulin Test ◽  
Cell Antibody ◽  
Antiglobulin Test ◽  
Low Ionic Strength ◽  
Enzyme Test ◽  
Screening Result

The aim of this study was to determine the frequencies and specificities of “enzyme-only” detected red blood cell (RBC) alloantibodies in the routine antibody screening and antibody identification in patients hospitalized in Austria. Routine blood samples of 2420 patients were investigated. The antibody screening was performed with a 3-cell panel in the low-ionic strength saline- (LISS-) indirect antiglobulin test (IAT) and with an enzyme-pretreated (papain) 3-cell panel fully automated on the ORTHO AutoVue Innova System. The antibody identification was carried out manually with an 11-cell panel in the LISS-IAT and with an enzyme-pretreated (papain) 11-cell panel. In total 4.05% (n=98) of all patients (n=2420) had a positive RBC antibody screening result. Of them 25.51% (25/98) showed “enzyme-only” detected specific or nonspecific RBC alloantibodies. Rhesus and Lewis system antibodies were found the only specificities of “enzyme-only” RBC alloantibodies: all in all 4.8% (4/98) were detected with anti-E, 3.06% (3/98) with anti-Lea, 3.06% (3/98) with anti-D after anti-D prophylaxis and 1.02% (1/98) with anti-e. In total, 14.29% (14/98) showed a nonspecific RBC alloantibody result with the enzyme test. The results of the present study demonstrate that a high number of unwanted positive reactions with the enzyme technique overshadows the detection of “enzyme-only” RBC alloantibodies. (Trial Registration: K-37-13).

scholarly journals Evaluation of 2-column agglutination versus conventional tube technique for antibody screening

2021 ◽  
Vol 9 (3) ◽  
pp. 407-412
Author(s):  
A. Abou Jabal ◽  
T. Shubeilat ◽  
F. Hajjiri
Keyword(s):  
Serum Samples ◽  
Antibody Screening ◽  
Negative Results ◽  
Specificity And Sensitivity ◽  
Low Concentrations ◽  
Indirect Antiglobulin Test ◽  
Antiglobulin Test ◽  
Screening And Identification ◽  
Clinically Significant ◽  
Positive Results

The study aimed to determine the specificity and sensitivity of the Ortho BioVue two-column agglutination system for the detection of low concentrations of clinically significant antibodies in serum. The BioVue system was compared with the conventional tube technique [LISS-Coombs indirect antiglobulin test], and the two-stage Papenzyme test was used to resolve discrepancies between the two methods. We tested 3000 serum samples from randomly selected patients at King Hussein Medical Centre. Both the antibody screening and identification gave negative results in 2952 patients and positive results in 48 patients. We found the BioVue system to be the more sensitive technique. However, if papain enzyme-treated cells were included in the conventional tube technique when applied to antibody screening and identification, both methods would be of comparable sensitivity

scholarly journals The importance of immunohematology testing in the neonatal period

2013 ◽  
Vol 66 (7-8) ◽  
pp. 317-321 ◽  
Author(s):  
Nevenka Bujandric ◽  
Mirjana Krga-Milanovic
Keyword(s):  
Blood Transfusion ◽  
Pregnant Women ◽  
Blood Group ◽  
Direct Antiglobulin Test ◽  
Hemolytic Disease ◽  
Blood Samples ◽  
Blood Types ◽  
Exchange Blood Transfusion ◽  
Indirect Antiglobulin Test ◽  
Antiglobulin Test

Introduction. In order to diagnose hemolytic disease of the newborn it is necessary to determine ABO/Rh blood group, direct antiglobulin test and indirect antiglobulin test in newborns as well as ABO/Rh and indirect antiglobulin test in their mothers due to a possible incompatibility between the blood types of the mother and her baby. The study was aimed at reviewing and analyzing the results of screening of the newborns and pregnant women on the territory of South Backa District during 2011, as well as at estimating the necessity to give blood transfusion to the newborns in the same period. Material and Methods. Data obtained from the information system and protocol of the Institute for Blood Transfusion of Vojvodina were used in a retrospective analysis of screening of newborns and pregnant women. Results. Blood samples taken from 3313 newborns were tested and the following distribution of the ABO blood types was recorded: O in 45.6%, A in 35%, B in 15.9%, in 3.5%; 78.9%, newborns were found to be D-positive, 21.1% were D-negative. The direct antiglobulin test was positive in 165 (4.9%) cases, it was weakly positive +/- in 85 (51.5%) , positive 1+ in 45 (27.3%), positive 2+ in 17 (10.3%), positive 3+ in 12 (7.3%), positive 4+ in 6 (3.6%). In all cases, the monospecific direct antiglobulin test was positive with IgG antibodies. Of four newborns with positive direct antiglobulin test who required exchange blood transfusion, two had RhD incompatibility and two had ABO incompatibility. Blood samples of 3429 (100%) women were tested and 36 (1.05%) were actively immunized: 23 (0.67%) to RhD antigen, 8 (0.23%) to blood group antigens other than ABH and Rh, specificity could not be determined in 5 (0.15%). Anti-D antibodies were found in 19 (0.55%) pregnant women, in two (0.06%) cases newborns required exchange blood transfusion. Conclusion. Testing neonates and pregnant women contributes to the detection of blood type incompatibility between the mother and her child; it provides an opportunity for clinicians to implement the adequate prevention of RhD alloimmunization as well as to make timely diagnosis and to introduce treatment of hemolytic disease in newborns.

scholarly journals USEFULNESS OF MONOCLONAL ANTI-HUMAN IgG REAGENT IN THE INDIRECT ANTIGLOBULIN TEST FOR A PATIENT WITH THE ANTI-JOHN MILTON HAGEN BLOOD GROUP

2018 ◽  
Vol 64 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Kimiyo Ogawa ◽  
Keiko Nagahama ◽  
Tatsuya Shiga ◽  
Takayuki Maruhashi ◽  
Akihiko Yokohama
Keyword(s):  
Blood Group ◽  
John Milton ◽  
Human Igg ◽  
Indirect Antiglobulin Test ◽  
Antiglobulin Test

scholarly journals Rare presentation of mixed autoimmune hemolytic anemia in children: Report of 2 cases

2017 ◽  
Vol 9 (04) ◽  
pp. 332-336 ◽  
Author(s):  
Preeti Rai ◽  
Geetika Sharma ◽  
Deeksha Singh ◽  
Jyoti Garg
Keyword(s):  
Blood Group ◽  
Hemolytic Anemia ◽  
Autoimmune Hemolytic Anemia ◽  
Drug Induced ◽  
Rare Presentation ◽  
Indirect Antiglobulin Test ◽  
Immune Hemolytic Anemia ◽  
Thermal Amplitude ◽  
Antiglobulin Test ◽  
Cross Match

AbstractImmune hemolytic anemia is characterized by clinical and laboratory features of hemolytic anemia with direct antiglobulin test (DAT) positivity. It could be autoimmune hemolytic anemia (AIHA), alloimmune, or drug-induced hemolysis based on the antigenic stimulus. Furthermore, based on thermal amplitude of autoantibody, AIHA is classified as warm (65%), cold (30%), and mixed (5%) type. Mixed AIHA is extremely rare in children and must be differentiated from warm AIHA with clinically insignificant cold agglutinins and cold hemagglutinin disease as their treatment is different. It may present as blood group discrepancy or cross-match incompatibility leading to delay in arranging suitable blood unit for transfusion. Therefore, a thorough immunohematology workup including monospecific DAT, indirect antiglobulin test at 4°C and 37°C, determination of thermal amplitude and titer is essential. We hereby present two pediatric cases of mixed AIHA presenting as ABO forward and reverse blood group discrepancy and cross-match incompatibility.

Blood Group Antibody Screening Tests during Pregnancy

1987 ◽  
Vol 42 (7) ◽  
pp. 432
Author(s):  
P. J. BOWELL ◽  
D. L. ALLEN ◽  
C. C. ENTWISTLE
Keyword(s):  
Blood Group ◽  
Screening Tests ◽  
Antibody Screening
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