scholarly journals Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Dietmar Enko ◽  
Claudia Habres ◽  
Franz Wallner ◽  
Barbara Mayr ◽  
Gabriele Halwachs-Baumann
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Samples ◽  
Antibody Screening ◽  
Indirect Antiglobulin Test ◽  
Cell Antibody ◽  
Antiglobulin Test ◽  
Low Ionic Strength ◽  
Enzyme Test ◽  
Screening Result

The aim of this study was to determine the frequencies and specificities of “enzyme-only” detected red blood cell (RBC) alloantibodies in the routine antibody screening and antibody identification in patients hospitalized in Austria. Routine blood samples of 2420 patients were investigated. The antibody screening was performed with a 3-cell panel in the low-ionic strength saline- (LISS-) indirect antiglobulin test (IAT) and with an enzyme-pretreated (papain) 3-cell panel fully automated on the ORTHO AutoVue Innova System. The antibody identification was carried out manually with an 11-cell panel in the LISS-IAT and with an enzyme-pretreated (papain) 11-cell panel. In total 4.05% (n=98) of all patients (n=2420) had a positive RBC antibody screening result. Of them 25.51% (25/98) showed “enzyme-only” detected specific or nonspecific RBC alloantibodies. Rhesus and Lewis system antibodies were found the only specificities of “enzyme-only” RBC alloantibodies: all in all 4.8% (4/98) were detected with anti-E, 3.06% (3/98) with anti-Lea, 3.06% (3/98) with anti-D after anti-D prophylaxis and 1.02% (1/98) with anti-e. In total, 14.29% (14/98) showed a nonspecific RBC alloantibody result with the enzyme test. The results of the present study demonstrate that a high number of unwanted positive reactions with the enzyme technique overshadows the detection of “enzyme-only” RBC alloantibodies. (Trial Registration: K-37-13).

scholarly journals Red blood cell alloimmunisation and autoimmunisation in transfusion dependent beta thalassemics from Southern India

2015 ◽  
Vol 05 (03) ◽  
pp. 004-008
Author(s):  
Mohammed Saleem E. K. ◽  
Soundarya Mahalingam ◽  
Shamee Shastri ◽  
Kamalakshi G. Bhat
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Beta Thalassemia ◽  
Red Cell ◽  
Transfusion Therapy ◽  
Female Ratio ◽  
Antibody Screening ◽  
Cell Antigen ◽  
Low Ionic Strength ◽  
Male To Female

AbstractThe development of red blood cell (RBC) isoimmunization with alloantibodies and autoantibodies complicate transfusion therapy in multiply transfused thalassemia patients. We conducted a study to analyse the frequency in our population. Clinical and antibody profile from 55 multiply transfused thalassemic patients who were receiving transfusions were collected and analyzed prospectively. A commercially available 3 cell antigen panel was used for the antibody screening procedure. If antibody screening with the 3-cell antigen panel was positive, an extended 11-cell antigen panel was used for antibody identification in LISS (Low Ionic Strength Solution). All patients received blood matched for only ABO and Rh (D) antigens. A total of 55 transfusion dependent â thalassemics were included in this study out of which 30 (54.55%) were males and 25(45.45%) females with a male to female ratio of 1.2: 1. Frequency of red cell alloimmunization in this study was found to be 1.8%. None of the patients developed red cell autoimmunization. The alloantibody identified in the the patient who developed alloimmunisation was was anti-K. In conclusion, the transfusion of matched blood is essential for chronically transfused beta thalassemia patients in order to avoid alloimmunization.

Red Blood Cell Antibody Screening with Groupamatic System

Vox Sanguinis ◽  
1981 ◽  
Vol 40 (1) ◽  
pp. 34-43 ◽  
Author(s):  
S. Confida ◽  
C. Hurel ◽  
N. Chesnel ◽  
M. Garretta ◽  
A. Muller
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Antibody Screening ◽  
Cell Antibody

Evaluation of a Decision Tree for Efficient Antenatal Red Blood Cell Antibody Screening

Epidemiology ◽  
2018 ◽  
Vol 29 (3) ◽  
pp. 453-457 ◽  
Author(s):  
Isabelle Le Ray ◽  
Brian Lee ◽  
Agneta Wikman ◽  
Marie Reilly
Keyword(s):  
Decision Tree ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Antibody Screening ◽  
Cell Antibody

Paper-based assay for red blood cell antigen typing by the indirect antiglobulin test

2016 ◽  
Vol 408 (19) ◽  
pp. 5231-5238 ◽  
Author(s):  
Natasha Yeow ◽  
Heather McLiesh ◽  
Liyun Guan ◽  
Wei Shen ◽  
Gil Garnier
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Antigen ◽  
Indirect Antiglobulin Test ◽  
Antiglobulin Test

Red Blood Cell Antibody Screening with Groupamatic System

Vox Sanguinis ◽  
1981 ◽  
Vol 40 (1) ◽  
pp. 34-43
Author(s):  
S. Confida ◽  
C. Hurel ◽  
N. Chesnel ◽  
M. Garretto ◽  
A. Muller
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Antibody Screening ◽  
Cell Antibody

scholarly journals Red blood cell antibody screening in pregnancy

2011 ◽  
Vol 5 (1) ◽  
pp. 56 ◽  
Author(s):  
ShanthalaA.M Devi ◽  
VanamalaA Alwar ◽  
S Sitalakshmi ◽  
Karuna Rameshkumar ◽  
Rita Mhaskar
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Antibody Screening ◽  
Cell Antibody ◽  
In Pregnancy

Real-time Red Blood Cell Counting and Osmolarity Analysis using a Photoacoustic-based Microfluidic System

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Wenxiu Zhao ◽  
Haibo Yu ◽  
Yangdong Wen ◽  
Hao Luo ◽  
Boliang Jia ◽  
...  
Keyword(s):  
Blood Cell ◽  
Physical Properties ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Diagnostic Procedure ◽  
Microfluidic System ◽  
Cell Counting ◽  
Blood Samples ◽  
System P ◽  
Blood Cell Counting

Counting the number of red blood cells (RBCs) in blood samples is a common clinical diagnostic procedure, but conventional methods are unable to provide the size and other physical properties...

In vitro effects of temperature on red blood cell deformability and membrane stability in human and various vertebrate species

2021 ◽  
pp. 1-10
Author(s):  
Adam Attila Matrai ◽  
Gabor Varga ◽  
Bence Tanczos ◽  
Barbara Barath ◽  
Adam Varga ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Whole Blood ◽  
Mechanical Stability ◽  
Cell Deformability ◽  
Blood Samples ◽  
Effects Of Temperature ◽  
The Way ◽  
Rbc Deformability

BACKGROUND: The effects of temperature on micro-rheological variables have not been completely revealed yet. OBJECTIVE: To investigate micro-rheological effects of heat treatment in human, rat, dog, and porcine blood samples. METHODS: Red blood cell (RBC) - buffer suspensions were prepared and immersed in a 37, 40, and 43°C heat-controlled water bath for 10 minutes. Deformability, as well as mechanical stability of RBCs were measured in ektacytometer. These tests were also examined in whole blood samples at various temperatures, gradually between 37 and 45°C in the ektacytometer. RESULTS: RBC deformability significantly worsened in the samples treated at 40 and 43°C degrees, more expressed in human, porcine, rat, and in smaller degree in canine samples. The way of heating (incubation vs. ektacytometer temperation) and the composition of the sample (RBC-PBS suspension or whole blood) resulted in the different magnitude of RBC deformability deterioration. Heating affected RBC membrane (mechanical) stability, showing controversial alterations. CONCLUSION: Significant changes occur in RBC deformability by increasing temperature, showing inter-species differences. The magnitude of alterations is depending on the way of heating and the composition of the sample. The results may contribute to better understanding the micro-rheological deterioration in hyperthermia or fever.

Unexpected red blood cell antibody distributions in Chinese people by a systematic literature review

Transfusion ◽  
2015 ◽  
Vol 56 (4) ◽  
pp. 975-979 ◽  
Author(s):  
Chunxia Chen ◽  
Jinzhe Tan ◽  
Lixin Wang ◽  
Bing Han ◽  
Wei Sun ◽  
...  
Keyword(s):  
Literature Review ◽  
Blood Cell ◽  
Systematic Literature Review ◽  
Red Blood Cell ◽  
Chinese People ◽  
Cell Antibody
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