Erythrocyte Antibody Screening in Solid-Phase: A Comparison of Two Solid-Phase Microplate Assays with the Indirect Antiglobulin Test in Polyethylene Glycol for the Detection of Irregular Erythrocyte Antibodies

Vox Sanguinis ◽  
1990 ◽  
Vol 59 (2) ◽  
pp. 96-100
Author(s):  
Corinne A.M. Hazenberg ◽  
Margriet B. Mulder ◽  
Josée M. Beelen
Keyword(s):  
Polyethylene Glycol ◽  
Solid Phase ◽  
Antibody Screening ◽  
Indirect Antiglobulin Test ◽  
Antiglobulin Test

scholarly journals Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Dietmar Enko ◽  
Claudia Habres ◽  
Franz Wallner ◽  
Barbara Mayr ◽  
Gabriele Halwachs-Baumann
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Samples ◽  
Antibody Screening ◽  
Indirect Antiglobulin Test ◽  
Cell Antibody ◽  
Antiglobulin Test ◽  
Low Ionic Strength ◽  
Enzyme Test ◽  
Screening Result

The aim of this study was to determine the frequencies and specificities of “enzyme-only” detected red blood cell (RBC) alloantibodies in the routine antibody screening and antibody identification in patients hospitalized in Austria. Routine blood samples of 2420 patients were investigated. The antibody screening was performed with a 3-cell panel in the low-ionic strength saline- (LISS-) indirect antiglobulin test (IAT) and with an enzyme-pretreated (papain) 3-cell panel fully automated on the ORTHO AutoVue Innova System. The antibody identification was carried out manually with an 11-cell panel in the LISS-IAT and with an enzyme-pretreated (papain) 11-cell panel. In total 4.05% (n=98) of all patients (n=2420) had a positive RBC antibody screening result. Of them 25.51% (25/98) showed “enzyme-only” detected specific or nonspecific RBC alloantibodies. Rhesus and Lewis system antibodies were found the only specificities of “enzyme-only” RBC alloantibodies: all in all 4.8% (4/98) were detected with anti-E, 3.06% (3/98) with anti-Lea, 3.06% (3/98) with anti-D after anti-D prophylaxis and 1.02% (1/98) with anti-e. In total, 14.29% (14/98) showed a nonspecific RBC alloantibody result with the enzyme test. The results of the present study demonstrate that a high number of unwanted positive reactions with the enzyme technique overshadows the detection of “enzyme-only” RBC alloantibodies. (Trial Registration: K-37-13).

scholarly journals Evaluation of 2-column agglutination versus conventional tube technique for antibody screening

2021 ◽  
Vol 9 (3) ◽  
pp. 407-412
Author(s):  
A. Abou Jabal ◽  
T. Shubeilat ◽  
F. Hajjiri
Keyword(s):  
Serum Samples ◽  
Antibody Screening ◽  
Negative Results ◽  
Specificity And Sensitivity ◽  
Low Concentrations ◽  
Indirect Antiglobulin Test ◽  
Antiglobulin Test ◽  
Screening And Identification ◽  
Clinically Significant ◽  
Positive Results

The study aimed to determine the specificity and sensitivity of the Ortho BioVue two-column agglutination system for the detection of low concentrations of clinically significant antibodies in serum. The BioVue system was compared with the conventional tube technique [LISS-Coombs indirect antiglobulin test], and the two-stage Papenzyme test was used to resolve discrepancies between the two methods. We tested 3000 serum samples from randomly selected patients at King Hussein Medical Centre. Both the antibody screening and identification gave negative results in 2952 patients and positive results in 48 patients. We found the BioVue system to be the more sensitive technique. However, if papain enzyme-treated cells were included in the conventional tube technique when applied to antibody screening and identification, both methods would be of comparable sensitivity

Evaluation of the polyethylene glycol-indirect antiglobulin test for routine compatibility testing

Transfusion ◽  
1989 ◽  
Vol 29 (8) ◽  
pp. 686-688 ◽  
Author(s):  
JL Slater ◽  
DJ Griswold ◽  
LS Wojtyniak ◽  
MJ Reisling
Keyword(s):  
Polyethylene Glycol ◽  
Indirect Antiglobulin Test ◽  
Antiglobulin Test

Droplet-based blood group antibody screening with laser incubation

The Analyst ◽  
2021 ◽  
Vol 146 (8) ◽  
pp. 2499-2505
Author(s):  
Clare A. Manderson ◽  
Heather McLiesh ◽  
Rico F. Tabor ◽  
Gil Garnier
Keyword(s):  
Blood Group ◽  
Hydrophobic Surface ◽  
Paper Strip ◽  
Antibody Screening ◽  
Indirect Antiglobulin Test ◽  
Antiglobulin Test

Complete indirect antiglobulin test (IAT) in a single diagnostic incorporating a laser-incubated droplet on a hydrophobic surface with read-out provided by a paper strip.

Electrospun Polyethylene Glycol/Polyvinyl Alcohol Composite Nanofibrous Membranes as Shape-Stabilized Solid–Solid Phase Change Materials

2020 ◽  
Vol 2 (3) ◽  
pp. 167-177 ◽  
Author(s):  
Junwen Huang ◽  
Houyong Yu ◽  
Somia Yassin Hussain Abdalkarim ◽  
Jaromir Marek ◽  
Jiri Militky ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Polyvinyl Alcohol ◽  
Phase Change ◽  
Phase Change Materials ◽  
Solid Phase ◽  
Nanofibrous Membranes
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