Rapid capture of biomolecules from blood via stimuli-responsive elastomeric particles for acoustofluidic separation

The Analyst ◽  
2020 ◽  
Vol 145 (24) ◽  
pp. 8087-8096
Author(s):  
Linying Li ◽  
C. Wyatt Shields ◽  
Jin Huang ◽  
Yiqun Zhang ◽  
Korine A. Ohiri ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Stimuli Responsive ◽  
Capture And Release ◽  
Detection Of Biomarkers ◽  
Elastomeric Particles

Detection of biomarkers often requires extensive sample preparation. We report a simple acoustofluidic method to isolate biomarkers on elastomeric particles by co-aggregation of elastin-like polypeptides, enabling their efficient capture and release.

scholarly journals Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics

Polymers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 267
Author(s):  
Chen Jiao ◽  
Franziska Obst ◽  
Martin Geisler ◽  
Yunjiao Che ◽  
Andreas Richter ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Disulfide Bonds ◽  
Stimuli Responsive ◽  
Disulfide Exchange ◽  
Protein Capture ◽  
Wide Range ◽  
Redox Responsive ◽  
Potential Applications ◽  
Capture And Release ◽  
Cross Linker

Stimuli-responsive hydrogels have a wide range of potential applications in microfluidics, which has drawn great attention. Double cross-linked hydrogels are very well suited for this application as they offer both stability and the required responsive behavior. Here, we report the integration of poly(N-isopropylacrylamide) (PNiPAAm) hydrogel with a permanent cross-linker (N,N′-methylenebisacrylamide, BIS) and a redox responsive reversible cross-linker (N,N′-bis(acryloyl)cystamine, BAC) into a microfluidic device through photopolymerization. Cleavage and re-formation of disulfide bonds introduced by BAC changed the cross-linking densities of the hydrogel dots, making them swell or shrink. Rheological measurements allowed for selecting hydrogels that withstand long-term shear forces present in microfluidic devices under continuous flow. Once implemented, the thiol-disulfide exchange allowed the hydrogel dots to successfully capture and release the protein bovine serum albumin (BSA). BSA was labeled with rhodamine B and functionalized with 2-(2-pyridyldithio)-ethylamine (PDA) to introduce disulfide bonds. The reversible capture and release of the protein reached an efficiency of 83.6% in release rate and could be repeated over 3 cycles within the microfluidic device. These results demonstrate that our redox-responsive hydrogel dots enable the dynamic capture and release of various different functionalized (macro)molecules (e.g., proteins and drugs) and have a great potential to be integrated into a lab-on-a-chip device for detection and/or delivery.

scholarly journals Stimuli responsive charge-switchable lipids: Capture and release of nucleic acids

2016 ◽  
Vol 196 ◽  
pp. 52-60 ◽  
Author(s):  
Joseph S. Hersey ◽  
Caroline M. LaManna ◽  
Hrvoje Lusic ◽  
Mark W. Grinstaff
Keyword(s):  
Nucleic Acids ◽  
Stimuli Responsive ◽  
Capture And Release

pH-Responsive magnetic nanospheres for the reversibly selective capture and release of glycoproteins

2017 ◽  
Vol 5 (6) ◽  
pp. 1236-1245 ◽  
Author(s):  
Qi Yang ◽  
Yue Zhu ◽  
Bin Luo ◽  
Fang Lan ◽  
Yao Wu ◽  
...  
Keyword(s):  
Biological Sample ◽  
High Selectivity ◽  
Stimuli Responsive ◽  
Ph Responsive ◽  
Protein Mixture ◽  
Magnetic Nanospheres ◽  
Protein Model ◽  
Pure Protein ◽  
Model Protein ◽  
Capture And Release

We present a pH-stimuli-responsive strategy to reversibly capture and release glycoproteins with high selectivity from a pure protein, model protein mixture and even a real biological sample.

scholarly journals Harnessing Peptide Binding to Capture and Reclaim Phosphate

2020 ◽  
Author(s):  
Whitney Fowler ◽  
Chuting Deng ◽  
Gabriella Griffen ◽  
Tess Teodoro ◽  
Ashley Z. Guo ◽  
...  
Keyword(s):  
Peptide Binding ◽  
Biological Processes ◽  
The Novel ◽  
Stimuli Responsive ◽  
Synthetic Material ◽  
Biologically Inspired ◽  
Dense Structure ◽  
New Class ◽  
Multiple Cycles ◽  
Capture And Release

With rising consumer demands, society is tapping into wastewater as an innovative source to recycle depleting resources. Novel reclamation technologies have been recently explored for this purpose, including several that optimize natural biological processes for targeted reclamation. However, this emerging field has a noticeable dearth of synthetic material technologies that are programmed to capture, release and recycle specified targets, and of the novel materials that do exist, synthetic platforms incorporating biologically inspired mechanisms are rare. We present here a prototype of a materials platform utilizing peptide amphiphiles that has been molecularly engineered to sequester, release, and reclaim phosphate utilizing a stimuli-responsive pH trigger, exploiting a protein-inspired binding mechanism that is incorporated directly into the self-assembled material network. This material is able to sequester completely and controllably release phosphate for multiple cycles of reuse. We have determined by simulations that the binding conformation of the peptide becomes constrained in the dense micelle corona at high pH such that phosphate is expelled when it otherwise would be preferentially bound. However, at neutral pH, this dense structure conversely employs multi-chain binding to further stabilize phosphate when it would otherwise be unbound, opening opportunities for higher-order conformational binding design to be engineered into this controllably packed corona. With this work, we are pioneering a new platform to be readily altered to capture other valuable targets, presenting a new class of capture and release materials for recycling resources on the nanoscale.

scholarly journals Assessment of Dimethoate in Olive Oil Samples Using a Dual Responsive Molecularly Imprinting-Based Approach

Foods ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 618
Author(s):  
Raquel Garcia ◽  
Elisabete P. Carreiro ◽  
João Carlos Lima ◽  
Marco Gomes da Silva ◽  
Ana Maria Costa Freitas ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Olive Oil ◽  
Functional Materials ◽  
Morphological Characterization ◽  
Advanced Materials ◽  
Stimuli Responsive ◽  
Molecularly Imprinted ◽  
Selective Enrichment ◽  
Dual Responsive ◽  
Preparation Step

A new generation of advanced materials developed by molecular imprinting technology showing a stimuli-responsive functionality are emerging. The switchable ability to control the uptake/release of the target analyte by action of external stimulus combined with a remarkable selectivity and specificity, makes these functional materials very attractive for sample preparation purposes. In this work, the usefulness of a sample preparation tool for the selective enrichment/pre-concentration of dimethoate from olive oil spiked samples based on “tailor-made” dual responsive magnetic and photonic molecularly imprinted polymers as sorbents is explored. To achieve this goal, a smart molecularly imprinted polymer (MIP) possessing magnetic and photonic responsiveness was successfully synthesized, and its physico-chemical and morphological characterization was assessed. Further, the trace analysis of dimethoate in spiked olive oil samples was validated and successfully implemented using smart-MIPs as sorbents in the sample preparation step, with high recoveries (83.5 ± 0.3%) and low detection limit (0.03 µg·mL−1).

Stimuli-Responsive Microfluidic Interface Enables Highly Efficient Capture and Release of Circulating Fetal Cells for Non-Invasive Prenatal Testing

2020 ◽  
Vol 92 (13) ◽  
pp. 9281-9286
Author(s):  
Huimin Zhang ◽  
Yuanyuan Yang ◽  
Yilong Liu ◽  
Yidi Wang ◽  
Weidong Ruan ◽  
...  
Keyword(s):  
Prenatal Testing ◽  
Stimuli Responsive ◽  
Fetal Cells ◽  
Non Invasive ◽  
Highly Efficient ◽  
Capture And Release

DEVELOPMENT OF IMMUNOCHEMICAL SYSTEMS TO CONTROL AUTHENTICITY AND COMPOSITION OF MEAT PRODUCTS

2021 ◽  
Vol 1 (19) ◽  
pp. 308-310
Author(s):  
E.A. Zvereva ◽  
O.D. Hendriсkson ◽  
D.S. Popravko ◽  
B.B. Dzantiev
Keyword(s):  
Heat Treatment ◽  
Sample Preparation ◽  
Meat Products ◽  
Selective Detection ◽  
Immunochromatographic Test ◽  
Technological Processes ◽  
Test Systems ◽  
Muscle Tissues ◽  
Detection Of Biomarkers

Immunochromatographic test systems with rapid sample preparation have been developed for testing the composition of meat products, which ensure the identification and assessment of the content of muscle tissues from different groups of animals. It was shown that the technological processes of enzymatic and heat treatment did not impair the selective detection of biomarkers.

scholarly journals Harnessing Peptide Binding to Capture and Reclaim Phosphate

2020 ◽  
Author(s):  
Whitney Fowler ◽  
Chuting Deng ◽  
Gabriella Griffen ◽  
Tess Teodoro ◽  
Ashley Z. Guo ◽  
...  
Keyword(s):  
Peptide Binding ◽  
Biological Processes ◽  
The Novel ◽  
Stimuli Responsive ◽  
Synthetic Material ◽  
Biologically Inspired ◽  
Dense Structure ◽  
New Class ◽  
Multiple Cycles ◽  
Capture And Release

With rising consumer demands, society is tapping into wastewater as an innovative source to recycle depleting resources. Novel reclamation technologies have been recently explored for this purpose, including several that optimize natural biological processes for targeted reclamation. However, this emerging field has a noticeable dearth of synthetic material technologies that are programmed to capture, release and recycle specified targets, and of the novel materials that do exist, synthetic platforms incorporating biologically inspired mechanisms are rare. We present here a prototype of a materials platform utilizing peptide amphiphiles that has been molecularly engineered to sequester, release, and reclaim phosphate utilizing a stimuli-responsive pH trigger, exploiting a protein-inspired binding mechanism that is incorporated directly into the self-assembled material network. This material is able to sequester completely and controllably release phosphate for multiple cycles of reuse. We have determined by simulations that the binding conformation of the peptide becomes constrained in the dense micelle corona at high pH such that phosphate is expelled when it otherwise would be preferentially bound. However, at neutral pH, this dense structure conversely employs multi-chain binding to further stabilize phosphate when it would otherwise be unbound, opening opportunities for higher-order conformational binding design to be engineered into this controllably packed corona. With this work, we are pioneering a new platform to be readily altered to capture other valuable targets, presenting a new class of capture and release materials for recycling resources on the nanoscale.

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