Advances in Molecularly Imprinting Technology for Bioanalytical Applications

In recent years, along with the rapid development of relevant biological fields, there has been a tremendous motivation to combine molecular imprinting technology (MIT) with biosensing. In this situation, bioprobes and biosensors based on molecularly imprinted polymers (MIPs) have emerged as a reliable candidate for a comprehensive range of applications, from biomolecule detection to drug tracking. Unlike their precursors such as classic immunosensors based on antibody binding and natural receptor elements, MIPs create complementary cavities with stronger binding affinity, while their intrinsic artificial polymers facilitate their use in harsh environments. The major objective of this work is to review recent MIP bioprobes and biosensors, especially those used for biomolecules and drugs. In this review, MIP bioprobes and biosensors are categorized by sensing method, including optical sensing, electrochemical sensing, gravimetric sensing and magnetic sensing, respectively. The working mechanism(s) of each sensing method are thoroughly discussed. Moreover, this work aims to present the cutting-edge structures and modifiers offering higher properties and performances, and clearly point out recent efforts dedicated to introduce multi-sensing and multi-functional MIP bioprobes and biosensors applicable to interdisciplinary fields.

Rapid and selective determination of melamine from eggs and milk based on magnetic surface molecularly imprinting technology

Fe3O4@SiO2@MIPs, which have strong magnetic responsiveness and high selectivity, were synthesized and applied towards the rapid extraction of MEL from milk and eggs.

NON CONVENTIONAL SURFACE FUNCTIONALIZATION OF POROUS POLY-ε-CAPROLACTONE SCAFFOLDS USING BIOACTIVE MOLECULARLY IMPRINTED NANOSPHERES

Polymeric scaffolds, in the form of porous membranes, modified to favour cell adhesion and proliferation on their surface, were prepared using an innovative technique of functionalization, based on molecularly imprinting technology. A series of membranes based on poly-ε-caprolactone were obtained through phase inversion using different solvents and non-solvents. Diffusion tests showed that prepared membranes exhibited a good permeability towards important nutrients such as folic acid and glucose. The ability of membranes, modified with imprinted nanoparticles, to facilitate cell adhesion and proliferation was evaluated by cell culture method using murine fibroblast cell line. SEM analysis at 72 h after seeding revealed an improved cell distribution on the surface of the membranes functionalized by deposition of molecularly imprinted nanoparticles.