pH-Responsive magnetic nanospheres for the reversibly selective capture and release of glycoproteins

2017 ◽  
Vol 5 (6) ◽  
pp. 1236-1245 ◽  
Author(s):  
Qi Yang ◽  
Yue Zhu ◽  
Bin Luo ◽  
Fang Lan ◽  
Yao Wu ◽  
...  
Keyword(s):  
Biological Sample ◽  
High Selectivity ◽  
Stimuli Responsive ◽  
Ph Responsive ◽  
Protein Mixture ◽  
Magnetic Nanospheres ◽  
Protein Model ◽  
Pure Protein ◽  
Model Protein ◽  
Capture And Release

We present a pH-stimuli-responsive strategy to reversibly capture and release glycoproteins with high selectivity from a pure protein, model protein mixture and even a real biological sample.

Soft stimuli-responsive grippers and machines with high load-to-weight ratios

2019 ◽  
Vol 6 (1) ◽  
pp. 160-168 ◽  
Author(s):  
Yajuan Sun ◽  
Linfeng Chen ◽  
Yan Jiang ◽  
Xuan Zhang ◽  
Xiukai Yao ◽  
...  
Keyword(s):  
General Class ◽  
High Load ◽  
Stimuli Responsive ◽  
Ph Responsive

A general class of stimuli-responsive grippers and actuators (e.g., temperature- and pH-responsive) with surprisingly high gripping strengths is introduced.

scholarly journals Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics

Polymers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 267
Author(s):  
Chen Jiao ◽  
Franziska Obst ◽  
Martin Geisler ◽  
Yunjiao Che ◽  
Andreas Richter ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Disulfide Bonds ◽  
Stimuli Responsive ◽  
Disulfide Exchange ◽  
Protein Capture ◽  
Wide Range ◽  
Redox Responsive ◽  
Potential Applications ◽  
Capture And Release ◽  
Cross Linker

Stimuli-responsive hydrogels have a wide range of potential applications in microfluidics, which has drawn great attention. Double cross-linked hydrogels are very well suited for this application as they offer both stability and the required responsive behavior. Here, we report the integration of poly(N-isopropylacrylamide) (PNiPAAm) hydrogel with a permanent cross-linker (N,N′-methylenebisacrylamide, BIS) and a redox responsive reversible cross-linker (N,N′-bis(acryloyl)cystamine, BAC) into a microfluidic device through photopolymerization. Cleavage and re-formation of disulfide bonds introduced by BAC changed the cross-linking densities of the hydrogel dots, making them swell or shrink. Rheological measurements allowed for selecting hydrogels that withstand long-term shear forces present in microfluidic devices under continuous flow. Once implemented, the thiol-disulfide exchange allowed the hydrogel dots to successfully capture and release the protein bovine serum albumin (BSA). BSA was labeled with rhodamine B and functionalized with 2-(2-pyridyldithio)-ethylamine (PDA) to introduce disulfide bonds. The reversible capture and release of the protein reached an efficiency of 83.6% in release rate and could be repeated over 3 cycles within the microfluidic device. These results demonstrate that our redox-responsive hydrogel dots enable the dynamic capture and release of various different functionalized (macro)molecules (e.g., proteins and drugs) and have a great potential to be integrated into a lab-on-a-chip device for detection and/or delivery.

scholarly journals Thermal versus Mechanical Unfolding in a Model Protein

2019 ◽  
Author(s):  
Rafael Tapia-Rojo ◽  
Juan J. Mazo ◽  
Fernando Falo
Keyword(s):  
Free Energy ◽  
Force Spectroscopy ◽  
Reaction Coordinate ◽  
Force Extension ◽  
Thermal Dynamics ◽  
Zero Force ◽  
Protein Model ◽  
Model Protein ◽  
State Models ◽  
Competing Pathways

Force spectroscopy techniques are often used to learn about the free energy landscape of single biomolecules, typically by recovering free energy quantities that, extrapolated to zero force, are compared to those measured in bulk experiments. However, it is not always clear how the information obtained from a mechanically perturbed system can be related to that obtained using other denaturants, since tensioned molecules unfold and refold along a reaction coordinate imposed by the force, which is unlikely meaningful in its absence. Here, we explore this dichotomy by investigating the unfolding landscape of a model protein, which is first unfolded mechanically through typical force spectroscopy-like protocols, and next thermally. When unfolded by non-equilibrium force extension and constant force protocols, we recover a simple two-barrier landscape, as the protein reaches the extended conformation through a metastable intermediate. Interestingly, folding-unfolding equilibrium simulations at low forces suggested a totally different scenario, where this metastable state plays little role in the unfolding mechanism, and the protein unfolds through two competing pathways27. Finally, we use Markov state models to describe the configurational space of the unperturbed protein close to the critical temperature. The thermal dynamics is well understood by a one-dimensional landscape along an appropriate reaction coordinate, however very different from the mechanical picture. In this sense, in our protein model the mechanical and thermal descriptions provide incompatible views of the folding/unfolding landscape of the system, and the estimated quantities to zero force result hard to interpret.

Microfluidic actuator based on stimuli-responsive hydrogel grafted into Cucurbita moschata xylems

Soft Matter ◽  
2021 ◽  
Author(s):  
Marcelo Ricardo Romero ◽  
Gisella Trejo ◽  
José Vedelago ◽  
Cesar Gomez
Keyword(s):  
Cucurbita Moschata ◽  
Stimuli Responsive ◽  
Ph Responsive ◽  
Chemically Modified ◽  
Responsive Polymer ◽  
Plant Stem

A chemical actuator was developed taking advantage of the internal microstructure of a plant stem. Stem xylems of Cucurbita moschata were chemically modified with a pH-responsive polymer to obtain an...

A stimuli responsive two component supramolecular hydrogelator with aggregation-induced emission properties

Soft Matter ◽  
2019 ◽  
Vol 15 (36) ◽  
pp. 7117-7121 ◽  
Author(s):  
Dennis Aschmann ◽  
Steffen Riebe ◽  
Thorben Neumann ◽  
Dennis Killa ◽  
Jan-Erik Ostwaldt ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Stimuli Responsive ◽  
Ph Responsive ◽  
Aggregation Induced Emission ◽  
Emission Properties ◽  
Two Component

A dual pH-responsive two component hydrogelator with aggregation-induced emission properties is described. The orchestration of supramolecular guadiniumcarbonylpyrrole dimerisation and the recognition of carboxylic acid is reason for the gelation.

scholarly journals Dual Stimuli-Responsive Copper Nanoparticles Decorated SBA-15: A Highly Efficient Catalyst for the Oxidation of Alcohols in Water

Nanomaterials ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 2051
Author(s):  
Anju Maria Thomas ◽  
Jerome Peter ◽  
Saravanan Nagappan ◽  
Anandhu Mohan ◽  
Chang-Sik Ha
Keyword(s):  
Mesoporous Silica ◽  
Copper Nanoparticles ◽  
Catalytic Performance ◽  
Solution Temperature ◽  
Stimuli Responsive ◽  
Hybrid Catalyst ◽  
Ph Responsive ◽  
Efficient Catalyst ◽  
13C Nuclear Magnetic Resonance ◽  
Alcohol Conversion

In the present work, a temperature and pH-responsive hybrid catalytic system using copolymer-capped mesoporous silica particles with metal nanoparticles is proposed. The poly(2-(dimethylamino)ethyl methacrylate)(DMAEMA)-co-N-tert-butyl acrylamide) (TBA)) shell on mesoporous silica SBA-15 was obtained through free radical polymerization. Then, copper nanoparticles (CuNPs) decorated SBA-15/copolymer hybrid materials were synthesized using the NaBH4 reduction method. SBA-15 was functionalized with trimethoxylsilylpropyl methacrylate (TMSPM) and named TSBA. It was found that the CuNPs were uniformly dispersed in the mesoporous channels of SBA-15, and the hybrid catalyst exhibited excellent catalytic performance for the selective oxidation of different substituted benzyl alcohols in water using H2O2 as an oxidant at room temperature. The dual (temperature and pH-) responsive behaviors of the CuNPs/p(DMAEMA-co-TBA)/TSBA catalyst were investigated using the dynamic light scattering technique. The conversion of catalytic products and selectivity were calculated using gas chromatographic techniques, whereas the molecular structure of the products was identified using 1H and 13C nuclear magnetic resonance (NMR) spectroscopy. The catalyst showed excellent catalytic activity toward the oxidation of alcohol to aldehyde in an aqueous medium below the lower critical solution temperature (LCST) and pKa values (7–7.5) of the copolymer. The main advantages of the hybrid catalyst, as compared to the existing catalysts, are outstanding alcohol conversion (up to 99%) for a short reaction time (1 h), small amount of the catalyst (5 mg), and good recyclability equal to at least five times.

scholarly journals Carbon Nano-Onions Reinforced Multilayered Thin Film System for Stimuli-Responsive Drug Release

Pharmaceutics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1208
Author(s):  
Narsimha Mamidi ◽  
Ramiro Velasco Delgadillo ◽  
Aldo Gonzáles Ortiz ◽  
Enrique Barrera
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Drug Release ◽  
Self Assembly ◽  
Critical Role ◽  
Layer By Layer ◽  
Film System ◽  
Stimuli Responsive ◽  
Ph Responsive ◽  
Thin Film System

Herein, poly (N-(4-aminophenyl) methacrylamide))-carbon nano-onions (PAPMA-CNOs = f-CNOs) and anilinated-poly (ether ether ketone) (AN-PEEK) have synthesized, and AN-PEEK/f-CNOs composite thin films were primed via layer-by-layer (LbL) self-assembly for stimuli-responsive drug release. The obtained thin films exhibited pH-responsive drug release in a controlled manner; pH 4.5 = 99.2% and pH 6.5 = 59.3% of doxorubicin (DOX) release was observed over 15 days. Supramolecular π-π stacking interactions between f-CNOs and DOX played a critical role in controlling drug release from thin films. Cell viability was studied with human osteoblast cells and augmented viability was perceived. Moreover, the thin films presented 891.4 ± 8.2 MPa of the tensile strength (σult), 43.2 ± 1.1 GPa of Young’s modulus (E), and 164.5 ± 1.7 Jg−1 of toughness (K). Quantitative scrutiny revealed that the well-ordered aligned nanofibers provide critical interphase, and this could be responsible for augmented tensile properties. Nonetheless, a pH-responsive and mechanically robust biocompatible thin-film system may show potential applications in the biomedical field.

Stimuli-Responsive Materials Applied as Carriers of Immobilized Enzymes

2009 ◽  
Vol 610-613 ◽  
pp. 1198-1202 ◽  
Author(s):  
Yu Zhang ◽  
Jing Liang Xu ◽  
Zhen Hong Yuan
Keyword(s):  
Steric Hindrance ◽  
Enzymatic Reaction ◽  
Immobilized Enzymes ◽  
Stimuli Responsive ◽  
Ph Responsive ◽  
Responsive Materials ◽  
Temperature Responsive ◽  
Activity Loss ◽  
Better Than

Enzymes, immobilized on pH/temperature/ion-responsive carriers, relieve poor contact and could be conveniently recycled as well. Enzymes were immobilized on pH-responsive carriers often by covalent method, which was better than non-covalent method (adsorption). The immobilized enzymes showed slight steric hindrance for enzymatic reaction, but always lose much activity during recycle process. The Km values were often increased significantly via immobilization on temperature/ion responsive carriers, while the immobilized enzymes expressed superior reusability. Both covalent and non-covalent methods were fit for enzymes to be immobilized on temperature-responsive carriers. Non-covalent method (entrapment) was preferential choose for enzymes to be immobilized on ion-responsive carriers. Many drawbacks, such as large activity loss and slow respondence during stimulus occurrence, seriously restrained the effect of the immobilized enzymes. It was supposed to open up special stimuli-responsive materials for enzymes immobilization, and choose proper immobilization methods, correspondingly.

scholarly journals Synthesis of surface capped mesoporous silica nanoparticles for pH-stimuli responsive drug delivery applications

MedChemComm ◽  
2017 ◽  
Vol 8 (9) ◽  
pp. 1797-1805 ◽  
Author(s):  
Madhappan Santha Moorthy ◽  
Subramanian Bharathiraja ◽  
Panchanathan Manivasagan ◽  
Kang Dae Lee ◽  
Junghwan Oh
Keyword(s):  
Drug Delivery ◽  
Cancer Therapy ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Drug Carrier ◽  
Stimuli Responsive ◽  
Ph Responsive ◽  
Drug Delivery Applications

Herein, we propose a “host–guest” complexation-based mesoporous silica drug carrier, MSNs@Mela@TTM, for pH-responsive drug delivery applications in cancer therapy.

Fabrication of pH-responsive monodisperse microcapsules using interfacial tension of immiscible phases

Soft Matter ◽  
2020 ◽  
Vol 16 (22) ◽  
pp. 5139-5147
Author(s):  
Dhawal R. Thakare ◽  
Grayson Schaer ◽  
Mostafa Yourdkhani ◽  
Nancy R. Sottos
Keyword(s):  
Interfacial Tension ◽  
Stimuli Responsive ◽  
Ph Responsive ◽  
Precise Control ◽  
Monodisperse Emulsion ◽  
Monodisperse Microcapsules

Monodisperse stimuli-responsive microcapsules are difficult to fabricate with precise control over capsule properties. The paper reports a facile technique to produce highly tunable and monodisperse emulsion-templated acid-responsive microcapsules.

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