A signal cascade amplification strategy based on RT-PCR triggering of a G-quadruplex DNAzyme for a novel electrochemical detection of viable Cronobacter sakazakii

The Analyst ◽  
2020 ◽  
Vol 145 (13) ◽  
pp. 4477-4483
Author(s):  
Yuanyuan Yuan ◽  
Xianyong Wu ◽  
Zhanmin Liu ◽  
Qiqi Ning ◽  
Liqiang Fu ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Rapid Detection ◽  
Detection Method ◽  
Cronobacter Sakazakii ◽  
Rt Pcr ◽  
Signal Cascade ◽  
G Quadruplex ◽  
Amplification Strategy ◽  
Cascade Amplification

An effective and sensitive DNAzyme method for electrochemical detection of viable Cronobacter sakazakii was designed. The detection method is based on RT-PCR and cascade amplification of ribozymes to achieve rapid detection of viable Cronobacter sakazakii.

A cascade signal amplification strategy for sensitive and label-free DNA detection based on Exo III-catalyzed recycling coupled with rolling circle amplification

The Analyst ◽  
2014 ◽  
Vol 139 (11) ◽  
pp. 2884-2889 ◽  
Author(s):  
Xingti Liu ◽  
Qingwang Xue ◽  
Yongshun Ding ◽  
Jing Zhu ◽  
Lei Wang ◽  
...  
Keyword(s):  
Detection Method ◽  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Label Free ◽  
Exonuclease Iii ◽  
Rolling Circle ◽  
Free Dna ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Cascade Amplification

A sensitive and label-free DNA detection method was developed based on cascade amplification combining exonuclease-III recycling with rolling circle amplification.

Simultaneous and rapid detection method for measles and rubella using single-tube multiplex real-time quantitative RT-PCR

2019 ◽  
Vol 25 (10) ◽  
pp. 829-831 ◽  
Author(s):  
Nori Yoshioka ◽  
Hideharu Hagiya ◽  
Matsuo Deguchi ◽  
Shigeto Hamaguchi ◽  
Masanori Kagita ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Detection Method ◽  
Rt Pcr ◽  
Single Tube

scholarly journals Visual Detection of Cucumber Green Mottle Mosaic Virus Based on Terminal Deoxynucleotidyl Transferase Coupled with DNAzymes Amplification

Sensors ◽  
2019 ◽  
Vol 19 (6) ◽  
pp. 1298 ◽  
Author(s):  
Ying Wang ◽  
Jing Liu ◽  
Hong Zhou
Keyword(s):  
Detection Limit ◽  
Mosaic Virus ◽  
Visual Detection ◽  
Detection Method ◽  
Plant Protection ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Stem Segment ◽  
Monitoring Time ◽  
G Quadruplex ◽  
Cascade Amplification

A simple, rapid, and sensitive visual detection method for observing cucumber green mottle mosaic virus was reported based on the template-independent polymerization activity of terminal deoxynucleotidyl transferase (TdT), coupled with the cascade amplification of Mg2+-dependent DNAzyme and hemin/G-quadruplex DNAzyme. Briefly, the hybridized dsDNA of T1/P1 was cut into two parts at its position of 5′-AA↓CG↑TT-3′ by the restricted enzyme AcII. The longer, newborn fragment originating from P1 was tailed at its 3’-end by oligo dG, and an intact enzymatic sequence of Mg2+-dependent DNAzyme was generated. The substrate sequence in the loop segment of the hairpin probe (HP) hybridized with the newborn enzymatic sequence and was cleaved into two parts in the presence of Mg2+. The locked G-quadruplex sequence in the stem segment of the HP was released, which catalyzed the oxidation of ABTS2- in the presence of H2O2, and the resulting solution turned green. A correlation between the absorbance and concentration of T1 was obtained in a range from 0.1 pM to 2 nM, with a detection limit of 0.1 pM. In addition to promoting a lower detection limit and shorter monitoring time, this method also demonstrated an excellent selectivity to single or double nucleotide changes. Therefore, the designed strategy provided a rapid and efficient platform for viral inspection and plant protection.

A label-free cyclic assembly of G-quadruplex nanowires for cascade amplification detection of T4 polynucleotide kinase activity and inhibition

The Analyst ◽  
2015 ◽  
Vol 140 (17) ◽  
pp. 6124-6130 ◽  
Author(s):  
Zhilu Shi ◽  
Xiafei Zhang ◽  
Rui Cheng ◽  
Baoxin Li ◽  
Yan Jin
Keyword(s):  
Kinase Activity ◽  
Hybridization Chain Reaction ◽  
Label Free ◽  
Chain Reaction ◽  
Polynucleotide Kinase ◽  
G Quadruplex ◽  
Amplification Strategy ◽  
Cascade Amplification ◽  
T4 Polynucleotide Kinase

A label-free and enzyme-free amplification strategy has been developed for sensitively and specifically studying PNK activity and inhibitionviathe hybridization chain reaction (HCR).

A single-tube RT-PCR for rapid detection and differentiation of some African isolates of palyam serogroup orbiviruses

2009 ◽  
Vol 161 (1) ◽  
pp. 70-74 ◽  
Author(s):  
Imadeldin E. Aradaib ◽  
Mohamed E.H. Mohamed ◽  
Mohamed A. Abdalla
Keyword(s):  
Rapid Detection ◽  
Rt Pcr ◽  
Single Tube
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