scholarly journals Visual Detection of Cucumber Green Mottle Mosaic Virus Based on Terminal Deoxynucleotidyl Transferase Coupled with DNAzymes Amplification

Sensors ◽  
2019 ◽  
Vol 19 (6) ◽  
pp. 1298 ◽  
Author(s):  
Ying Wang ◽  
Jing Liu ◽  
Hong Zhou
Keyword(s):  
Detection Limit ◽  
Mosaic Virus ◽  
Visual Detection ◽  
Detection Method ◽  
Plant Protection ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Stem Segment ◽  
Monitoring Time ◽  
G Quadruplex ◽  
Cascade Amplification

A simple, rapid, and sensitive visual detection method for observing cucumber green mottle mosaic virus was reported based on the template-independent polymerization activity of terminal deoxynucleotidyl transferase (TdT), coupled with the cascade amplification of Mg2+-dependent DNAzyme and hemin/G-quadruplex DNAzyme. Briefly, the hybridized dsDNA of T1/P1 was cut into two parts at its position of 5′-AA↓CG↑TT-3′ by the restricted enzyme AcII. The longer, newborn fragment originating from P1 was tailed at its 3’-end by oligo dG, and an intact enzymatic sequence of Mg2+-dependent DNAzyme was generated. The substrate sequence in the loop segment of the hairpin probe (HP) hybridized with the newborn enzymatic sequence and was cleaved into two parts in the presence of Mg2+. The locked G-quadruplex sequence in the stem segment of the HP was released, which catalyzed the oxidation of ABTS2- in the presence of H2O2, and the resulting solution turned green. A correlation between the absorbance and concentration of T1 was obtained in a range from 0.1 pM to 2 nM, with a detection limit of 0.1 pM. In addition to promoting a lower detection limit and shorter monitoring time, this method also demonstrated an excellent selectivity to single or double nucleotide changes. Therefore, the designed strategy provided a rapid and efficient platform for viral inspection and plant protection.

A G-quadruplex DNAzyme-based LAMP biosensing platform for a novel colorimetric detection ofListeria monocytogenes

2018 ◽  
Vol 10 (8) ◽  
pp. 848-854 ◽  
Author(s):  
Zhanmin Liu ◽  
Chenhui Yao ◽  
Yanming Wang ◽  
Cuiyun Yang
Keyword(s):  
Listeria Monocytogenes ◽  
Visual Detection ◽  
Colorimetric Detection ◽  
G Quadruplex ◽  
Oflisteria Monocytogenes ◽  
Cascade Amplification

A LAMP-based method for the visual detection ofListeria monocytogeneshas been developed by employing DNAzyme-catalyzed cascade amplification of the colorimetric signal.

A signal cascade amplification strategy based on RT-PCR triggering of a G-quadruplex DNAzyme for a novel electrochemical detection of viable Cronobacter sakazakii

The Analyst ◽  
2020 ◽  
Vol 145 (13) ◽  
pp. 4477-4483
Author(s):  
Yuanyuan Yuan ◽  
Xianyong Wu ◽  
Zhanmin Liu ◽  
Qiqi Ning ◽  
Liqiang Fu ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Rapid Detection ◽  
Detection Method ◽  
Cronobacter Sakazakii ◽  
Rt Pcr ◽  
Signal Cascade ◽  
G Quadruplex ◽  
Amplification Strategy ◽  
Cascade Amplification

An effective and sensitive DNAzyme method for electrochemical detection of viable Cronobacter sakazakii was designed. The detection method is based on RT-PCR and cascade amplification of ribozymes to achieve rapid detection of viable Cronobacter sakazakii.

Alcohol Determination by HPLC with Postcolumn Derivatization Based on the Thiosulfate-catalyzed Reaction of Alcohols and Cerium(IV) and Fluorescence Detection of Cerium(III)

2020 ◽  
Vol 16 ◽  
Author(s):  
Ikko Mikami ◽  
Eri Shibayama ◽  
Kengo Takagi
Keyword(s):  
Detection Limit ◽  
Fluorescence Detection ◽  
Reaction Rate ◽  
Detection Method ◽  
Fluorescence Detector ◽  
Reaction Solution ◽  
Optimum Detection ◽  
Postcolumn Derivatization ◽  
Sensitive Method

Background: Determination of a reducing substance based on the reaction between Ce(IV) and a reducing substance and fluorescence detection of Ce(III) generated has been reported as a selective and sensitive method. However, this method could not be applied to the determination of alcohol due to the low reaction rate of alcohol and Ce(IV). Objective: We found that thiosulfate catalytically enhanced reaction of alcohols (such as, methanol, ethanol, and propanol) and Ce(IV). Utilizing this effect, we developed a new method for the determination of alcohols. Results: In the presence of thiosulfate, an increase in fluorescence intensity was detected by injecting alcohol at concentrations of several millimolar, whereas it was not observed even at the concentration of 10% v/v (2 M for ethanol) in the absence of thiosulfate. The optimum detection conditions were determined to be 4.0 mM Ce(IV) sulfate and 0.50 mM thiosulfate, and the detection limit (S/N = 3) of ethanol under these conditions was 1 mM. In the calibration curves, changes in the slope were observed when the alcohol concentrations were approximately 10–25 mM. Using a thiosulfate solution containing ethanol as the reaction solution, a calibration curve without any change in slope was obtained, although the concentration of ethanol at the detection limit increased. The alcohols in the liquor and fuel were successfully analyzed using the proposed detection method as a postcolumn reaction. Conclusion: This new alcohol detection method using a versatile fluorescence detector can be applied to the postcolumn reaction of HPLC omitting need of time-consuming pretreatment processes.

scholarly journals Topical Application of Double-Stranded RNA Targeting 2b and CP Genes of Cucumber mosaic virus Protects Plants against Local and Systemic Viral Infection

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 963
Author(s):  
Maria C. Holeva ◽  
Athanasios Sklavounos ◽  
Rajendran Rajeswaran ◽  
Mikhail M. Pooggin ◽  
Andreas E. Voloudakis
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Topical Application ◽  
Plant Virus ◽  
Plant Protection ◽  
Post Inoculation ◽  
Tobacco Plants ◽  
Double Stranded Rna

Cucumber mosaic virus (CMV) is a destructive plant virus with worldwide distribution and the broadest host range of any known plant virus, as well as a model plant virus for understanding plant–virus interactions. Since the discovery of RNA interference (RNAi) as a major antiviral defense, RNAi-based technologies have been developed for plant protection against viral diseases. In plants and animals, a key trigger of RNAi is double-stranded RNA (dsRNA) processed by Dicer and Dicer-like (DCL) family proteins in small interfering RNAs (siRNAs). In the present study, dsRNAs for coat protein (CP) and 2b genes of CMV were produced in vitro and in vivo and applied onto tobacco plants representing a systemic solanaceous host as well as on a local host plant Chenopodium quinoa. Both dsRNA treatments protected plants from local and systemic infection with CMV, but not against infection with unrelated viruses, confirming sequence specificity of antiviral RNAi. Antiviral RNAi was effective when dsRNAs were applied simultaneously with or four days prior to CMV inoculation, but not four days post inoculation. In vivo-produced dsRNAs were more effective than the in vitro-produced; in treatments with in vivo dsRNAs, dsRNA-CP was more effective than dsRNA-2b, while the effects were opposite with in vitro dsRNAs. Illumina sequencing of small RNAs from in vivo dsRNA-CP treated and non-treated tobacco plants revealed that interference with CMV infection in systemic leaves coincides with strongly reduced accumulation of virus-derived 21- and 22-nucleotide (nt) siRNAs, likely generated by tobacco DCL4 and DCL2, respectively. While the 21-nt class of viral siRNAs was predominant in non-treated plants, 21-nt and 22-nt classes accumulated at almost equal (but low) levels in dsRNA treated plants, suggesting that dsRNA treatment may boost DCL2 activity. Taken together, our findings confirm the efficacy of topical application of dsRNA for plant protection against viruses and shed more light on the mechanism of antiviral RNAi.

Target-controlled gating liposome “off–on” cascade amplification for sensitive and accurate detection of phospholipase D in breast cancer cells with a low-background signal

2016 ◽  
Vol 52 (70) ◽  
pp. 10660-10663 ◽  
Author(s):  
Qingwang Xue ◽  
Wei Jiang ◽  
Lei Wang
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Phospholipase D ◽  
Breast Cancer Cells ◽  
Detection Method ◽  
Background Signal ◽  
Low Background ◽  
Accurate Detection ◽  
Cascade Amplification

Here we developed a simple, sensitive and accurate PLD detection method based on a target-controlled gating liposome (TCGL) “off–on” cascade amplified strategy and personal glucose meters (PGMs).

A label-free biosensor for selective detection of DNA and Pb2+ based on a G-quadruplex

RSC Advances ◽  
2016 ◽  
Vol 6 (10) ◽  
pp. 7765-7771 ◽  
Author(s):  
Ruipeng Hou ◽  
Xiaoqing Niu ◽  
Fengling Cui
Keyword(s):  
Detection Method ◽  
Selective Detection ◽  
Label Free ◽  
Schematic Representation ◽  
G Quadruplex

Schematic representation of the DNA and Pb2+ detection method.

scholarly journals Multispectral visual detection method for conveyor belt longitudinal tear

Measurement ◽  
2019 ◽  
Vol 143 ◽  
pp. 246-257 ◽  
Author(s):  
Chengcheng Hou ◽  
Tiezhu Qiao ◽  
Haitao Zhang ◽  
Yusong Pang ◽  
Xiaoyan Xiong
Keyword(s):  
Visual Detection ◽  
Detection Method ◽  
Conveyor Belt ◽  
Longitudinal Tear

scholarly journals Computer Vision Measurement of Pointer Meter Readings Based on Inverse Perspective Mapping

2019 ◽  
Vol 9 (18) ◽  
pp. 3729 ◽  
Author(s):  
Bao ◽  
Tan ◽  
Liu ◽  
Miao
Keyword(s):  
Computer Vision ◽  
Visual Detection ◽  
Detection Method ◽  
Transformation Matrix ◽  
Normal Vector ◽  
Vision Measurement ◽  
Extrinsic Parameters ◽  
Perspective Effect ◽  
Rotation Transformation ◽  
Inverse Perspective Mapping

A computer vision method for measuring multiple pointer meters is proposed based on the inverse perspective mapping. First, the measured meter scales are used as the calibration objects to obtain the extrinsic parameters of the meter plane. Second, normal vector of the meter plane can be acquired by the extrinsic parameters, obtaining the rotation transformation matrix of the meter image. Then, the acquired meter image is transformed to a position both parallel to the meter plane and near the main point by the rotation transformation matrix and the extrinsic parameters, eliminating the perspective effect of the acquired image. Finally, the transformed image is tested by the visual detection method to obtain the readings of the pointer meter, improving measurement precision. The results of the measurement verify the effectiveness and accuracy of the method.

Label-free and sensitive detection of coralyne and heparin based on target-induced G-quadruplex formation

2019 ◽  
Vol 11 (10) ◽  
pp. 1331-1337 ◽  
Author(s):  
Mingjian Chen ◽  
Changbei Ma ◽  
Han Zhao ◽  
Kemin Wang
Keyword(s):  
Detection Method ◽  
Sensitive Detection ◽  
Label Free ◽  
G Quadruplex ◽  
Quadruplex Formation

Herein we propose a label-free and sensitive detection method for coralyne and heparin, based on utilizing the complex of adenosine16 (A16) and coralyne to induce the formation of a G-quadruplex scaffold.

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