A cascade signal amplification strategy for sensitive and label-free DNA detection based on Exo III-catalyzed recycling coupled with rolling circle amplification

The Analyst ◽  
2014 ◽  
Vol 139 (11) ◽  
pp. 2884-2889 ◽  
Author(s):  
Xingti Liu ◽  
Qingwang Xue ◽  
Yongshun Ding ◽  
Jing Zhu ◽  
Lei Wang ◽  
...  
Keyword(s):  
Detection Method ◽  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Label Free ◽  
Exonuclease Iii ◽  
Rolling Circle ◽  
Free Dna ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Cascade Amplification

A sensitive and label-free DNA detection method was developed based on cascade amplification combining exonuclease-III recycling with rolling circle amplification.

Target-responsive dumbbell probe-mediated rolling circle amplification strategy for highly sensitive Hg2+ detection

RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 27091-27097 ◽  
Author(s):  
Qingwang Xue ◽  
Yanqin Lv ◽  
Yuanfu Zhang ◽  
Shuling Xu ◽  
Qiaoli Yue ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Highly Sensitive ◽  
Mercuric Ions ◽  
Amplification Strategy

A novel label-free amplified fluorescent sensing scheme based on target-responsive dumbbell probe-mediated rolling circle amplification (D-RCA) has been developed for sensitive and selective detection of mercuric ions.

Label-free DNA detection based on oligonucleotide-stabilized silver nanoclusters and exonuclease III-catalyzed target recycling amplification

2014 ◽  
Vol 6 (15) ◽  
pp. 6082-6087 ◽  
Author(s):  
Hui Ma ◽  
Wei Wei ◽  
Qian Lu ◽  
Zhixin Zhou ◽  
Henan Li ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Free Dna ◽  
Sensitivity And Selectivity ◽  
Exo Iii ◽  
Ag Ncs ◽  
Recycling Amplification

A label-free DNA biosensor with high sensitivity and selectivity is constructed by using DNA–Ag NCs and Exo III-catalyzed target recycling amplification.

A low-cost, label-free DNA detection method in lab-on-chip format based on electrohydrodynamic instabilities, with application to long-range PCR

Lab on a Chip ◽  
2012 ◽  
Vol 12 (22) ◽  
pp. 4738 ◽  
Author(s):  
Mohamed Lemine Youba Diakité ◽  
Jerôme Champ ◽  
Stephanie Descroix ◽  
Laurent Malaquin ◽  
François Amblard ◽  
...  
Keyword(s):  
Long Range ◽  
Detection Method ◽  
Dna Detection ◽  
Low Cost ◽  
Label Free ◽  
Lab On Chip ◽  
Free Dna ◽  
Chip Format ◽  
Long Range Pcr ◽  
On Chip

A ratiometric electrochemical biosensor for ultrasensitive and highly selective detection of the K-ras gene via exonuclease III-assisted target recycling and rolling circle amplification strategies

2019 ◽  
Vol 11 (32) ◽  
pp. 4146-4156 ◽  
Author(s):  
Qi Xiao ◽  
Jinrong Feng ◽  
Jiawen Li ◽  
Yi Liu ◽  
Dan Wang ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Ras Gene ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Rolling Circle ◽  
Exo Iii

A ratiometric electrochemical biosensor for ultrasensitive and highly selective detection of the K-ras gene via Exo III-assisted target recycling and RCA strategies.

Label-free detection of nicotinamide adenine dinucleotide based on ligation-triggered exonuclease III-assisted signal amplification

RSC Advances ◽  
2015 ◽  
Vol 5 (105) ◽  
pp. 86625-86630 ◽  
Author(s):  
Haiyan Zhao ◽  
Lei Wang ◽  
Xingti Liu ◽  
Zhiyue Gao ◽  
Wei Jiang
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Signal Amplification ◽  
Nicotinamide Adenine ◽  
Label Free ◽  
Exonuclease Iii ◽  
Label Free Detection ◽  
Exo Iii ◽  
Amplification Strategy

Schematic illustration of the Exo III-assisted amplification strategy for NAD+ detection.

Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4267-4272 ◽  
Author(s):  
Biqing Bao ◽  
Yanrui Pan ◽  
Bingbing Gu ◽  
Jia Chen ◽  
Yu Xu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Conjugated Polyelectrolytes ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Exo Iii ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Cascade Amplification

A ratiometric and cascade amplification strategy that combines the signal amplification and effecitive FRET property of CPEs with the Exo III-assisted target recycling method has been developed for DNA detection.

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