Red light-triggered nucleic acid-templated reaction based on cyclic oligonucleotide substrates

2019 ◽  
Vol 55 (72) ◽  
pp. 10713-10716 ◽  
Author(s):  
Oleksii Zozulia ◽  
Tobias Bachmann ◽  
Nina S. Deussner-Helfmann ◽  
Frank Beierlein ◽  
Mike Heilemann ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Chemical Reaction ◽  
Red Light ◽  
Specific Detection

We developed a red light-triggered, fluorogenic chemical reaction based on cyclic oligonucleotide substrates that is accelerated over 30-fold by nucleic acid templates that allows quick and sequence specific detection of nucleic acid down to 1 nM.

A nucleic acid-directed, red light-induced chemical reaction

2010 ◽  
Vol 46 (31) ◽  
pp. 5659 ◽  
Author(s):  
Annabelle Fülöp ◽  
Xiaohua Peng ◽  
Marc M. Greenberg ◽  
Andriy Mokhir
Keyword(s):  
Nucleic Acid ◽  
Chemical Reaction ◽  
Red Light

scholarly journals Rapid and specific detection of Salmonella infections using chemically modified nucleic acid probes

2019 ◽  
Vol 1054 ◽  
pp. 157-166 ◽  
Author(s):  
Isabel Machado ◽  
Victoria Garrido ◽  
Luiza I. Hernandez ◽  
Juliana Botero ◽  
Nora Bastida ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Specific Detection ◽  
Nucleic Acid Probes ◽  
Chemically Modified ◽  
Salmonella Infections

scholarly journals Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection

2018 ◽  
Vol 9 (4) ◽  
pp. 896-903 ◽  
Author(s):  
Jessica Sayers ◽  
Richard J. Payne ◽  
Nicolas Winssinger
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Specific Detection ◽  
Ligation Reaction ◽  
Low Concentrations ◽  
Mirna Detection ◽  
Peptide Ligation

A PNA-templated peptide ligation reaction has been developed between selenocystine and selenoesters. The methodology was used for the sequence specific detection of miRNA at low concentrations.

Magnetic colloids: preparation and biomedical applications

e-Polymers ◽  
2005 ◽  
Vol 5 (1) ◽  
Author(s):  
Abdelhamid Elaissari
Keyword(s):  
Nucleic Acid ◽  
Biomedical Applications ◽  
Colloidal Particles ◽  
Solid Support ◽  
Diagnostic Methods ◽  
Nucleic Acid Detection ◽  
Specific Detection ◽  
Biomedical Diagnosis ◽  
Biomedical Diagnostics ◽  
Limited Sensitivity

AbstractIncreasing interest has been focused on the preparation of magnetic latex particles and their use in biomedical diagnostics. All elaborated magnetic colloidal particles or latexes are principally used as a solid support of biomolecules involved in the specific capture of targeted biomolecules such as antigens in immunoassays (i.e., ELISA) and nucleic acid detection in ELOSA. Nowadays, the main objective in biomedical diagnosis is to enhance the sensitivity and to accelerate the procedure by elaborating new magnetic colloidal particles and diagnostic methods. The general problem in this field is related to the limited sensitivity. One possibility to partially solve it is to increase the concentration of captured targets (antigens, nucleic acids, etc.) before the specific detection step. Thus, the use of appropriate magnetic colloidal particles and methods for extraction and concentration of biomolecules is of extreme interest.

Sign in / Sign up

Export Citation Format

Share Document