scholarly journals Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection

2018 ◽  
Vol 9 (4) ◽  
pp. 896-903 ◽  
Author(s):  
Jessica Sayers ◽  
Richard J. Payne ◽  
Nicolas Winssinger
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Specific Detection ◽  
Ligation Reaction ◽  
Low Concentrations ◽  
Mirna Detection ◽  
Peptide Ligation

A PNA-templated peptide ligation reaction has been developed between selenocystine and selenoesters. The methodology was used for the sequence specific detection of miRNA at low concentrations.

scholarly journals A new colorimetric peptide nucleic acid-based assay for the specific detection of bacteria

2014 ◽  
Vol 9 (10) ◽  
pp. 1131-1142
Author(s):  
Carina Almeida ◽  
Diogo Constante ◽  
André Ferreira ◽  
Laura Cerqueira ◽  
Maria J Vieira ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Specific Detection

scholarly journals Effect of Chlorine on Incorporation of Helicobacter pylori into Drinking Water Biofilms

2009 ◽  
Vol 76 (5) ◽  
pp. 1669-1673 ◽  
Author(s):  
M. S. Gi�o ◽  
N. F. Azevedo ◽  
S. A. Wilks ◽  
M. J. Vieira ◽  
C. W. Keevil
Keyword(s):  
Helicobacter Pylori ◽  
Drinking Water ◽  
Nucleic Acid ◽  
Pure Culture ◽  
Peptide Nucleic Acid ◽  
Culture Methods ◽  
Low Concentrations ◽  
H Pylori ◽  
Specific Peptide ◽  
Effect Of Chlorine

ABSTRACT The use of a specific peptide nucleic acid (PNA) probe demonstrated that Helicobacter pylori persisted inside biofilms exposed to low concentrations of chlorine (0.2 and 1.2 mg liter−1) for at least 26 days, although no culturable cells were recovered. Coupled with data obtained using viability stains in pure culture, this result suggests that H. pylori can survive chlorination but remain undetectable by culture methods, which can be effectively replaced by PNA hybridization.

scholarly journals Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Cronobacter Genomospecies (Enterobacter sakazakii) in Powdered Infant Formula

2009 ◽  
Vol 75 (9) ◽  
pp. 2925-2930 ◽  
Author(s):  
C. Almeida ◽  
N. F. Azevedo ◽  
C. Iversen ◽  
S. Fanning ◽  
C. W. Keevil ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Infant Formula ◽  
Peptide Nucleic Acid ◽  
Powdered Infant Formula ◽  
Enterobacter Sakazakii ◽  
Specific Detection ◽  
Peptide Nucleic Acid Probe ◽  
Bacterial Contaminants ◽  
Specificity And Sensitivity

ABSTRACT Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.

scholarly journals Design of Tail-Clamp Peptide Nucleic Acid Tethered with Azobenzene Linker for Sequence-Specific Detection of Homopurine DNA

Molecules ◽  
2017 ◽  
Vol 22 (11) ◽  
pp. 1840 ◽  
Author(s):  
Shinjiro Sawada ◽  
Toshifumi Takao ◽  
Nobuo Kato ◽  
Kunihiro Kaihatsu
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Specific Detection

Synergy of Peptide–Nucleic Acid and Spherical Nucleic Acid Enabled Quantitative and Specific Detection of Tumor Exosomal MicroRNA

2019 ◽  
Vol 91 (20) ◽  
pp. 13198-13205 ◽  
Author(s):  
Li Liu ◽  
Hao Lu ◽  
Ruixue Shi ◽  
Xin-Xin Peng ◽  
Qingwei Xiang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Specific Detection ◽  
Spherical Nucleic Acid ◽  
Exosomal Microrna

scholarly journals Highly sensitive and specific screening of EGFR mutation using a PNA microarray-based fluorometric assay based on rolling circle amplification and graphene oxide

RSC Advances ◽  
2019 ◽  
Vol 9 (66) ◽  
pp. 38298-38308 ◽  
Author(s):  
Xiaojun Xu ◽  
Shu Xing ◽  
Mengjia Xu ◽  
Pan Fu ◽  
Tingting Gao ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Egfr Mutation ◽  
Rolling Circle Amplification ◽  
Specific Detection ◽  
Fluorometric Method ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
Detection Probe

A facile peptide nucleic acid microarray-based fluorometric method was developed for sensitive and specific detection of EGFR mutation by using rolling circle amplification, graphene oxide, and a fluorescently-labeled detection probe.

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