A nucleic acid-directed, red light-induced chemical reaction

2010 ◽  
Vol 46 (31) ◽  
pp. 5659 ◽  
Author(s):  
Annabelle Fülöp ◽  
Xiaohua Peng ◽  
Marc M. Greenberg ◽  
Andriy Mokhir
Keyword(s):  
Nucleic Acid ◽  
Chemical Reaction ◽  
Red Light

Red light-triggered nucleic acid-templated reaction based on cyclic oligonucleotide substrates

2019 ◽  
Vol 55 (72) ◽  
pp. 10713-10716 ◽  
Author(s):  
Oleksii Zozulia ◽  
Tobias Bachmann ◽  
Nina S. Deussner-Helfmann ◽  
Frank Beierlein ◽  
Mike Heilemann ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Chemical Reaction ◽  
Red Light ◽  
Specific Detection

We developed a red light-triggered, fluorogenic chemical reaction based on cyclic oligonucleotide substrates that is accelerated over 30-fold by nucleic acid templates that allows quick and sequence specific detection of nucleic acid down to 1 nM.

scholarly journals Nucleic Acid Templated Chemical Reaction in a Live Vertebrate

2016 ◽  
Vol 2 (6) ◽  
pp. 394-400 ◽  
Author(s):  
Laurent Holtzer ◽  
Igor Oleinich ◽  
Marcello Anzola ◽  
Eric Lindberg ◽  
Kalyan K. Sadhu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Chemical Reaction

Porphyrin-based photosensitizers and their DNA conjugates for singlet oxygen induced nucleic acid interstrand crosslinking

2017 ◽  
Vol 15 (25) ◽  
pp. 5402-5409 ◽  
Author(s):  
Eva M. Llamas ◽  
João P. C. Tome ◽  
João M. M. Rodrigues ◽  
Tomás Torres ◽  
Annemieke Madder
Keyword(s):  
Nucleic Acid ◽  
Singlet Oxygen ◽  
Red Light ◽  
Dna Conjugates ◽  
Interstrand Crosslink ◽  
Interstrand Crosslinking

Porphyrin-based photosensitisers and their DNA conjugates have been evaluated for interstrand crosslink generation using furan containing oligonucleotides and red light.

Red light-triggered photoreduction on a nucleic acid template

2020 ◽  
Vol 56 (69) ◽  
pp. 10026-10029
Author(s):  
Subrata Dutta ◽  
Jennifer Rühle ◽  
Margot Schikora ◽  
Nina Deussner-Helfmann ◽  
Mike Heilemann ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Red Light ◽  
Pyropheophorbide A ◽  
Azobenzene Derivatives

Conjugate Sn(iv)(pyropheophorbide a)dichloride–(peptide nucleic acid) catalyzes reduction of azobenzene derivatives in the presence of complementary nucleic acid (NA) upon irridiation with red light (660 nm)

Introduction

1978 ◽  
Vol 36 (3) ◽  
pp. 543-544
Author(s):  
W. Bernard
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Nucleic Acid ◽  
Gene Mapping ◽  
Molecular Genetics ◽  
Cell Biology ◽  
Gene Activity ◽  
Close Connection ◽  
Basic Information ◽  
Simple Systems

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.

Infection of Escherichia coli with bacteriophages

1969 ◽  
Vol 27 ◽  
pp. 370-371
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Nucleic Acid ◽  
Cell Surface ◽  
Virus Type ◽  
Infection Process ◽  
Adsorption Site ◽  
The Other ◽  
Specific Receptors ◽  
Bacterial Receptors

The first step in the infection of a bacterium by a virus consists of a collision between cell and bacteriophage. The presence of virus-specific receptors on the cell surface will trigger a number of events leading eventually to release of the phage nucleic acid. The execution of the various "steps" in the infection process varies from one virus-type to the other, depending on the anatomy of the virus. Small viruses like ØX 174 and MS2 adsorb directly with their capsid to the bacterial receptors, while other phages possess attachment organelles of varying complexity. In bacteriophages T3 (Fig. 1) and T7 the small conical processes of their heads point toward the adsorption site; a welldefined baseplate is attached to the head of P22; heads without baseplates are not infective.

Nucleic Acid Molecules Prepared by Monolayer Techniques

1972 ◽  
Vol 30 ◽  
pp. 178-179
Author(s):  
Dimitrij Lang
Keyword(s):  
Electron Microscopy ◽  
Standard Deviation ◽  
Nucleic Acid ◽  
Cytochrome C ◽  
Nucleic Acids ◽  
Contour Length ◽  
Sample Standard Deviation ◽  
Molecular Weights ◽  
Length Measurements ◽  
Protein Monolayer

The success of the protein monolayer technique for electron microscopy of individual DNA molecules is based on the prevention of aggregation and orientation of the molecules during drying on specimen grids. DNA adsorbs first to a surface-denatured, insoluble cytochrome c monolayer which is then transferred to grids, without major distortion, by touching. Fig. 1 shows three basic procedures which, modified or not, permit the study of various important properties of nucleic acids, either in concert with other methods or exclusively:1) Molecular weights relative to DNA standards as well as number distributions of molecular weights can be obtained from contour length measurements with a sample standard deviation between 1 and 4%.

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