Linker-free antibody conjugation for sensitive hydrogel microparticle-based multiplex immunoassay

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6712-6720
Author(s):  
Hyun Jee Lee ◽  
Ju Yeon Kim ◽  
Yoon Ho Roh ◽  
Sun Min Kim ◽  
Ki Wan Bong
Keyword(s):  
Multiplex Immunoassay ◽  
Hydrogel Microparticles ◽  
Highly Sensitive ◽  
Free Antibody ◽  
Antibody Conjugation ◽  
Hydrogel Microparticle

Graphically encoded hydrogel microparticles were directly conjugated with reduced antibodies without linkers for highly sensitive multiplex immunoassay.

scholarly journals Highly sensitive label-free antibody detection using a long period fibre grating sensor

2018 ◽  
Vol 271 ◽  
pp. 24-32 ◽  
Author(s):  
LiangLiang Liu ◽  
Leonel Marques ◽  
Ricardo Correia ◽  
Stephen P. Morgan ◽  
Seung-Woo Lee ◽  
...  
Keyword(s):  
Antibody Detection ◽  
Label Free ◽  
Long Period ◽  
Highly Sensitive ◽  
Free Antibody

scholarly journals Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography

2020 ◽  
Vol 9 (2) ◽  
pp. 301
Author(s):  
Nak Jun Lee ◽  
Sejung Maeng ◽  
Hyeon Ung Kim ◽  
Yoon Ho Roh ◽  
Changhyun Hwang ◽  
...  
Keyword(s):  
Fluorescence Analysis ◽  
Medical Intervention ◽  
Breast Cancer Cell Lines ◽  
Invasive Technique ◽  
Cell Capture ◽  
Hydrogel Microparticles ◽  
Hydrogel Particles ◽  
Antibody Conjugation ◽  
Cell Affinity ◽  
Novel Method

Technologies for the detection and isolation of circulating tumor cells (CTCs) are essential in liquid biopsy, a minimally invasive technique for early diagnosis and medical intervention in cancer patients. A promising method for CTC capture, using an affinity-based approach, is the use of functionalized hydrogel microparticles (MP), which have the advantages of water-like reactivity, biologically compatible materials, and synergy with various analysis platforms. In this paper, we demonstrate the feasibility of CTC capture by hydrogel particles synthesized using a novel method called degassed mold lithography (DML). This technique increases the porosity and functionality of the MPs for effective conjugation with antibodies. Qualitative fluorescence analysis demonstrates that DML produces superior uniformity, integrity, and functionality of the MPs, as compared to conventional stop flow lithography (SFL). Analysis of the fluorescence intensity from porosity-controlled MPs by each reaction step of antibody conjugation elucidates that more antibodies are loaded when the particles are more porous. The feasibility of selective cell capture is demonstrated using breast cancer cell lines. In conclusion, using DML for the synthesis of porous MPs offers a powerful method for improving the cell affinity of the antibody-conjugated MPs.

scholarly journals Microparticle parking and isolation for highly sensitive microRNA detection

Lab on a Chip ◽  
2017 ◽  
Vol 17 (18) ◽  
pp. 3120-3128 ◽  
Author(s):  
Jae Jung Kim ◽  
Lynna Chen ◽  
Patrick S. Doyle
Keyword(s):  
Microfluidic Platform ◽  
Hydrogel Microparticles ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Monodisperse Droplets

We demonstrate a microfluidic platform that immobilizes hydrogel microparticles at defined locations, and encapsulates particles in monodisperse droplets for sensitive bioassays.

scholarly journals A Highly Sensitive and Specific SARS-CoV-2 Spike- and Nucleoprotein-Based Fluorescent Multiplex Immunoassay (FMIA) to Measure IgG, IgA, and IgM Class Antibodies

2021 ◽  
Author(s):  
Anna Solastie ◽  
Camilla Virta ◽  
Anu Haveri ◽  
Nina Ekström ◽  
Anu Kantele ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Clinical Performance ◽  
Neutralizing Antibody ◽  
Serological Assay ◽  
Multiplex Immunoassay ◽  
Reliable Estimation ◽  
Highly Sensitive ◽  
Antibody Titers

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serological assays with excellent clinical performance are essential for reliable estimation of the persistence of immunity after infection or vaccination. In this paper we present a thoroughly validated SARS-CoV-2 serological assay with excellent clinical performance and good comparability to neutralizing antibody titers.

Multiplex immunoassay of chicken cytokines via highly-sensitive chemiluminescent imaging array

2019 ◽  
Vol 1049 ◽  
pp. 213-218 ◽  
Author(s):  
Yihong Zhong ◽  
Xinyue Wu ◽  
Juan Li ◽  
Qingchun Lan ◽  
Qingling Jing ◽  
...  
Keyword(s):  
Multiplex Immunoassay ◽  
Imaging Array ◽  
Highly Sensitive

scholarly journals Hydrogel Microparticles Functionalized with Engineered Escherichia coli as Living Lactam Biosensors

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5556 ◽  
Author(s):  
Conghui Ma ◽  
Jie Li ◽  
Boyin Zhang ◽  
Chenxi Liu ◽  
Jingwei Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Flow Cytometry ◽  
Metabolic Engineering ◽  
High Throughput ◽  
Droplet Microfluidics ◽  
Dependent Manner ◽  
Hydrogel Microparticles ◽  
Commodity Chemicals ◽  
Hydrogel Microparticle ◽  
Dose Dependent

Recently there has been an increasing need for synthesizing valued chemicals through biorefineries. Lactams are an essential family of commodity chemicals widely used in the nylon industry with annual production of millions of tons. The bio-production of lactams can substantially benefit from high-throughput lactam sensing strategies for lactam producer screening. We present here a robust and living lactam biosensor that is directly compatible with high-throughput analytical means. The biosensor is a hydrogel microparticle encapsulating living microcolonies of engineered lactam-responsive Escherichia coli. The microparticles feature facile and ultra-high throughput manufacturing of up to 10,000,000 per hour through droplet microfluidics. We show that the biosensors can specifically detect major lactam species in a dose-dependent manner, which can be quantified using flow cytometry. The biosensor could potentially be used for high-throughput metabolic engineering of lactam biosynthesis.

scholarly journals Multiplex Immunoassay Techniques for On-Site Detection of Security Sensitive Toxins

Toxins ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 727
Author(s):  
Christopher Pöhlmann ◽  
Thomas Elßner
Keyword(s):  
Food Poisoning ◽  
Natural Food ◽  
Low Molecular Weight ◽  
Multiplex Detection ◽  
Multiplex Immunoassay ◽  
Highly Sensitive ◽  
Botulinum Neurotoxins ◽  
Living Organisms ◽  
Site Identification ◽  
Detection Technologies

Biological toxins are a heterogeneous group of high molecular as well as low molecular weight toxins produced by living organisms. Due to their physical and logistical properties, biological toxins are very attractive to terrorists for use in acts of bioterrorism. Therefore, among the group of biological toxins, several are categorized as security relevant, e.g., botulinum neurotoxins, staphylococcal enterotoxins, abrin, ricin or saxitoxin. Additionally, several security sensitive toxins also play a major role in natural food poisoning outbreaks. For a prompt response to a potential bioterrorist attack using biological toxins, first responders need reliable, easy-to-use and highly sensitive methodologies for on-site detection of the causative agent. Therefore, the aim of this review is to present on-site immunoassay platforms for multiplex detection of biological toxins. Furthermore, we introduce several commercially available detection technologies specialized for mobile or on-site identification of security sensitive toxins.

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