scholarly journals Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography

2020 ◽  
Vol 9 (2) ◽  
pp. 301
Author(s):  
Nak Jun Lee ◽  
Sejung Maeng ◽  
Hyeon Ung Kim ◽  
Yoon Ho Roh ◽  
Changhyun Hwang ◽  
...  
Keyword(s):  
Fluorescence Analysis ◽  
Medical Intervention ◽  
Breast Cancer Cell Lines ◽  
Invasive Technique ◽  
Cell Capture ◽  
Hydrogel Microparticles ◽  
Hydrogel Particles ◽  
Antibody Conjugation ◽  
Cell Affinity ◽  
Novel Method

Technologies for the detection and isolation of circulating tumor cells (CTCs) are essential in liquid biopsy, a minimally invasive technique for early diagnosis and medical intervention in cancer patients. A promising method for CTC capture, using an affinity-based approach, is the use of functionalized hydrogel microparticles (MP), which have the advantages of water-like reactivity, biologically compatible materials, and synergy with various analysis platforms. In this paper, we demonstrate the feasibility of CTC capture by hydrogel particles synthesized using a novel method called degassed mold lithography (DML). This technique increases the porosity and functionality of the MPs for effective conjugation with antibodies. Qualitative fluorescence analysis demonstrates that DML produces superior uniformity, integrity, and functionality of the MPs, as compared to conventional stop flow lithography (SFL). Analysis of the fluorescence intensity from porosity-controlled MPs by each reaction step of antibody conjugation elucidates that more antibodies are loaded when the particles are more porous. The feasibility of selective cell capture is demonstrated using breast cancer cell lines. In conclusion, using DML for the synthesis of porous MPs offers a powerful method for improving the cell affinity of the antibody-conjugated MPs.

Linker-free antibody conjugation for sensitive hydrogel microparticle-based multiplex immunoassay

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6712-6720
Author(s):  
Hyun Jee Lee ◽  
Ju Yeon Kim ◽  
Yoon Ho Roh ◽  
Sun Min Kim ◽  
Ki Wan Bong
Keyword(s):  
Multiplex Immunoassay ◽  
Hydrogel Microparticles ◽  
Highly Sensitive ◽  
Free Antibody ◽  
Antibody Conjugation ◽  
Hydrogel Microparticle

Graphically encoded hydrogel microparticles were directly conjugated with reduced antibodies without linkers for highly sensitive multiplex immunoassay.

Electronic Stool (e-Stool): A Novel Self-Stabilizing Video Capsule Endoscope for Reliable Non-Invasive Colonic Imaging

2011 ◽  
Author(s):  
Dobromir Filip ◽  
Marjan Eggermont ◽  
Jacquelyn Nagel ◽  
C. N. Andrews ◽  
Orly Yadid-Pecht ◽  
...  
Keyword(s):  
Final Stage ◽  
Invasive Technique ◽  
Gi Tract ◽  
Expansion Process ◽  
Natural Processes ◽  
Non Invasive ◽  
Novel Method ◽  
Biomimetic Approach ◽  
Capsule Size ◽  
Human Stool

Video capsule endoscopy (VCE) has become a popular non-invasive technique to study the small intestine. However, colonic VCE has been problematic due to capsule tumbling in the larger lumen of this organ. Self-stabilizing VCE is a novel method to visualize the colon without tumbling utilizing a biomimetic approach. The proposed design uses the free energy of the body’s natural processes employed to move chyme, and imitates the formation and propagation of stool. In its final stage, it physically and mechanically mimics natural feces. The process starts by administering the capsule orally. The capsule size, shape, and material were chosen to provide a smooth transit throughout the gastrointestinal (GI) tract. Once it reaches the colon, its special outer casing enzymatically dissolves. A stabilizing component that is attached to the back end of the capsule starts quickly expanding in the cecum by osmosis. This increase of the volumetric size of the expandable component (stabilizing component) invokes natural peristalsis by colonic mass reflex. Since the expansion process takes place very quickly, the capsule gets stabilized before the expansion-provoked peristalsis starts. At the final stage, the artificially created expanded component (behaving like an artificial stool) centralizes the capsule during its voyage in the colon, allowing a very smooth transit due to its viscosity. The aim of the present study is to present the design of the capsule from a biomimetic perspective and to comparatively quantify the mechanical properties of the design with those of actual human stool.

scholarly journals Uncovering friction dynamics using hydrogel particles as soft ball bearings

Soft Matter ◽  
2020 ◽  
Vol 16 (15) ◽  
pp. 3821-3831
Author(s):  
Raisa E. D. Rudge ◽  
Jesse P. M. van de Sande ◽  
Joshua A. Dijksman ◽  
Elke Scholten
Keyword(s):  
Ball Bearings ◽  
Hydrogel Microparticles ◽  
Rate Dependent ◽  
Hydrogel Particles ◽  
Friction Dynamics

Hydrogel microparticles transition through four rate-dependent regimes.

scholarly journals Flexible Octopus-Shaped Hydrogel Particles for Specific Cell Capture

Small ◽  
2016 ◽  
Vol 12 (15) ◽  
pp. 2001-2008 ◽  
Author(s):  
Lynna Chen ◽  
Harry Z. An ◽  
Ramin Haghgooie ◽  
Aaron T. Shank ◽  
Joseph M. Martel ◽  
...  
Keyword(s):  
Specific Cell ◽  
Cell Capture ◽  
Hydrogel Particles

scholarly journals Analysis of Foot Ulcer Level Using Non-Invasive Technique Based on Image Segmentation for Type 2 Diabetic Patients

2020 ◽  
pp. 430-434
Author(s):  
Tamilselvan K S ◽  
Sumithra M G ◽  
Murugeasan G ◽  
Balaji Sathyamoorthy ◽  
Rajeshwari Hegde
Keyword(s):  
Image Segmentation ◽  
Foot Ulcer ◽  
Clinical Analysis ◽  
Assessment Method ◽  
Medical Intervention ◽  
Invasive Technique ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Empirical Observation ◽  
Foot Ulceration

Medical imaging is that the technique and method of making visual representations of the inside of a body for clinical analysis and medical intervention, furthermore as visual illustration of the performance of some organs or tissues (physiology). In computer vision, image segmentation is the method of partitioning a digital image into its constituent parts with identical pixel values. The goal of segmentation is to modify the illustration of a picture into one thing that is a lot of purposeful and easier to investigate. A foot ulceration is associate degree open sore on the foot. Foot ulceration may be a shallow red crater that involves solely the surface skin. Foot ulceration can also be terribly deep. There are many issues with current practices for treating diabetic foot ulcers. First, patients should move to their wound clinic on a daily basis to own their wounds checked by their clinicians. Second, a clinician’s wound assessment method is predicated on visual examination. Technology using image analysis techniques may be a potential resolution to each these issues. The wound image is captured by the camera on the smart phone with the help of a picture capture box. In the Image pre-processing step, the electronic image is first down-sampled into high-resolution image. Second, the pictures are sleeked to get rid of noise (which is assumed primarily to be Gaussian noise made by image acquisition process) by mistreatment the Gaussian blur methodology whose variance with 0.5 was through empirical observation judged to be optimum supported multiple experiments. The foot outline is decided by using Sobel edge detection methodology. Then, Region of interest is developed by keeping the foot as foreground and rest as background. Color segmentation is performed by partitioning pixels into totally different clusters supported color similarity and abstraction relation. The RYB image is transformed to HSV and color thresholding is performed to find the outer area.

Beyond Cell Capture: Antibody Conjugation Improves Hemocompatibility for Vascular Tissue Engineering Applications

2010 ◽  
Vol 16 (8) ◽  
pp. 2485-2495 ◽  
Author(s):  
Mark Seow Khoon Chong ◽  
Swee-Hin Teoh ◽  
Erin Yiling Teo ◽  
Zhi-Yong Zhang ◽  
Chueng Neng Lee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Cell Capture ◽  
Engineering Applications ◽  
Antibody Conjugation ◽  
Capture Antibody

Total reflection X-ray fluorescence analysis of aerosol particles with direct dissolution of the collection filter on a substrate

2021 ◽  
Author(s):  
Tsugufumi Matsuyama ◽  
Hiroki Yamaguchi ◽  
Kouichi Tsuji
Keyword(s):  
Trace Elements ◽  
Aerosol Particles ◽  
Fluorescence Analysis ◽  
Total Reflection ◽  
X Ray ◽  
Novel Method ◽  
Direct Dissolution

In this study, a novel method based on total reflection X-ray analysis was developed for determining the trace elements in aerosol particles.

scholarly journals Catastrophe Disguised as a Complication After Epiduroscopy-A Case Study

2021 ◽  
pp. 1-5
Author(s):  
Eva M. Monzon
Keyword(s):  
Clinical History ◽  
Neurological Complications ◽  
Medical Intervention ◽  
Lower Limbs ◽  
Invasive Technique ◽  
Interventional Treatment ◽  
Key Words Complications ◽  
Surgical Interventions ◽  
Lower Limb Pain ◽  
Left Limb

Background: Epiduroscopy is a minimally invasive technique that enables diagnosis and treatment within the epidural space with direct vision. Previously reported complications of this technique have been mostly mild neurological complications of a transitory nature. In the present case, we describe a serious complication following an epiduroscopy, resulting in death. Case Report: The patient was a 39-year-old woman with lower back and lower limb pain who had undergone 3 surgical interventions previously on the lumbar spine. Only limited results from conservative and interventional treatment had been observed. She underwent an epiduroscopy in the Pain Management Unit. Immediately after the procedure, which was completed without incident, the patient presented neurological symptoms with areflexic paraplegia and a loss of sensation in the lower limbs and the upper left limb. Imaging tests identified signs of craniospinal hypotension that progressed slowly despite medical intervention. Ventricular dilation and cerebral edema without remission occurred, which ultimately caused the patient’s death 2 months after admission to the intensive care unit. Conclusion: Epiduroscopy has the potential to present adverse effects and complications. A thorough evaluation of the clinical history and the imaging tests are advisable Key words: Complications, epiduroscopy, low back pain, spinal cord, spinal endoscopy

Novel method for measuring junctional proton permeation in isolated ventricular myocyte cell pairs

2004 ◽  
Vol 287 (5) ◽  
pp. H2352-H2363 ◽  
Author(s):  
Pawel Swietach ◽  
Richard D. Vaughan-Jones
Keyword(s):  
Ventricular Myocytes ◽  
Reaction Model ◽  
Weak Acid ◽  
Invasive Technique ◽  
Guinea Pig Heart ◽  
Simple Method ◽  
Proton Permeability ◽  
Single Ventricular Myocytes ◽  
A Cell ◽  
Novel Method

Partial exposure of single ventricular myocytes to membrane-permeant weak acids or bases, using a dual-microperfusion technique, generates large and stable intracellular pH (pHi) gradients. In this study, we have investigated the feasibility of using the technique to estimate junctional proton permeability. This was done by recording the pHi gradient developed across the junctional region of a pair of conjoined ventricular myocytes, isolated enzymically from a guinea pig heart when one of the cells was partially exposed to acetate or ammonium. We show that under HEPES-buffered conditions, the junctional discontinuity in the pHi profile can be used to derive an apparent proton permeability coefficient ( PHapp). The mean PHapp obtained was 4.45 ± 0.21·10−4 cm/s ( n = 43) at an average junctional pHi of 7.04 ± 0.02. In the presence of the junctional inhibitor α-glycyrrhetinic acid, exposure of the proximal cell to weak acid or base produced no pHi change in the distal cell, confirming that distal changes were normally caused by acid-base flux through connexons assembled into junctional channels. The validity of the dual-microperfusion method was tested further by using a diffusion-permeation-reaction model for intracellular protons, designed to highlight possible errors in the estimates of PHapp. Our technique for measuring PHapp provides a useful alternative to the previous, more invasive technique of locally loading acid through a cell-attached patch pipette. The technique may provide a simple method for investigating the factors regulating cell-to-cell proton transmission.

scholarly journals Assessment of a novel method to detect clarithromycin-resistant Helicobacter pylori using a stool antigen test reagent

2020 ◽  
Author(s):  
Toshihiko Kakiuchi ◽  
Kazutoshi Hashiguchi ◽  
Ichiro Imamura ◽  
Aiko Nakayama ◽  
Ayako Takamori ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Nested Pcr ◽  
Invasive Technique ◽  
Antigen Test ◽  
Resistance Mutations ◽  
Stool Antigen Test ◽  
Urease Test ◽  
Stool Antigen ◽  
Novel Method ◽  
H Pylori

Abstract Background The resistance rate of Helicobacter pylori to clarithromycin (CAM) is high among the infected children in Japan; therefore, a new method for detecting CAM-resistant H. pylori using a less invasive technique is strongly desired. We aimed to confirm the clinical usefulness of our newly developed Nested polymerase chain reaction-QProbe (quenching probe) (Nested PCR-QP) method using stool specimens. Methods The first was an evaluation of our method using the residual solution of the H. pylori stool antigen test for adolescents, and the second was an evaluation of our method using culture testing for adults. Results Of the 57 middle school students with H. pylori , the Nested PCR-QP test results of 53 (90.3%) could be analyzed; 28 students were found to have CAM resistance mutations. The results indicating genetic mutation in 28 and no mutation in 23 students were consistent with those of PCR-direct sequencing. In the 23 adults who were diagnosed with H. pylori infection using the rapid urease test and culture testing, it was possible to use Nested PCR-QP for analyzing all 21 adults who tested positive in the stool H. pylori antigen test. The results obtained for all the 21 adults were consistent with those obtained via the drug susceptibility test. Conclusions Our novel method could be useful for non-invasively detecting CAM resistance mutations in H. pylori. This may help select an eradication drug to reduce eradication failure rates against H. pylori.

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