scholarly journals Microparticle parking and isolation for highly sensitive microRNA detection

Lab on a Chip ◽  
2017 ◽  
Vol 17 (18) ◽  
pp. 3120-3128 ◽  
Author(s):  
Jae Jung Kim ◽  
Lynna Chen ◽  
Patrick S. Doyle
Keyword(s):  
Microfluidic Platform ◽  
Hydrogel Microparticles ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Monodisperse Droplets

We demonstrate a microfluidic platform that immobilizes hydrogel microparticles at defined locations, and encapsulates particles in monodisperse droplets for sensitive bioassays.

A fully integrated microfluidic platform for highly sensitive analysis of immunochemical parameters

The Analyst ◽  
2017 ◽  
Vol 142 (22) ◽  
pp. 4206-4214 ◽  
Author(s):  
Sascha Lutz ◽  
Eloisa Lopez-Calle ◽  
Pamela Espindola ◽  
Christoph Boehm ◽  
Thorsten Brueckner ◽  
...  
Keyword(s):  
Cardiac Markers ◽  
Immunochemical Analysis ◽  
Sensitive Analysis ◽  
Microfluidic Platform ◽  
Fully Integrated ◽  
Highly Sensitive

A fully integrated cartridge for highly sensitive immunochemical analysis of cardiac markers with new microfluidic functionalities is presented.

scholarly journals Highly sensitive dual mode electrochemical platform for microRNA detection

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Pawan Jolly ◽  
Marina R. Batistuti ◽  
Anna Miodek ◽  
Pavel Zhurauski ◽  
Marcelo Mulato ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Dual Mode ◽  
Electrode Surfaces ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Detection Mode ◽  
Amplification Strategy

Abstract MicroRNAs (miRNAs) play crucial regulatory roles in various human diseases including cancer, making them promising biomarkers. However, given the low levels of miRNAs present in blood, their use as cancer biomarkers requires the development of simple and effective analytical methods. Herein, we report the development of a highly sensitive dual mode electrochemical platform for the detection of microRNAs. The platform was developed using peptide nucleic acids as probes on gold electrode surfaces to capture target miRNAs. A simple amplification strategy using gold nanoparticles has been employed exploiting the inherent charges of the nucleic acids. Electrochemical impedance spectroscopy was used to monitor the changes in capacitance upon any binding event, without the need for any redox markers. By using thiolated ferrocene, a complementary detection mode on the same sensor was developed where the increasing peaks of ferrocene were recorded using square wave voltammetry with increasing miRNA concentration. This dual-mode approach allows detection of miRNA with a limit of detection of 0.37 fM and a wide dynamic range from 1 fM to 100 nM along with clear distinction from mismatched target miRNA sequences. The electrochemical platform developed can be easily expanded to other miRNA/DNA detection along with the development of microarray platforms.

Linker-free antibody conjugation for sensitive hydrogel microparticle-based multiplex immunoassay

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6712-6720
Author(s):  
Hyun Jee Lee ◽  
Ju Yeon Kim ◽  
Yoon Ho Roh ◽  
Sun Min Kim ◽  
Ki Wan Bong
Keyword(s):  
Multiplex Immunoassay ◽  
Hydrogel Microparticles ◽  
Highly Sensitive ◽  
Free Antibody ◽  
Antibody Conjugation ◽  
Hydrogel Microparticle

Graphically encoded hydrogel microparticles were directly conjugated with reduced antibodies without linkers for highly sensitive multiplex immunoassay.

Accelerated DNAzyme-based fluorescent nanoprobe for highly sensitive microRNA detection in live cells

2020 ◽  
Vol 56 (3) ◽  
pp. 470-473 ◽  
Author(s):  
Yanan Wu ◽  
Hong-Min Meng ◽  
Juan Chen ◽  
Kemei Jiang ◽  
Ran Yang ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Live Cells ◽  
Fluorescent Nanoprobe ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

An accelerated DNAzyme-based fluorescent nanoprobe was developed for rapid and highly sensitive detection of microRNA in live cells.

Post-synthesis functionalized hydrogel microparticles for high performance microRNA detection

2019 ◽  
Vol 1076 ◽  
pp. 110-117 ◽  
Author(s):  
Yoon Ho Roh ◽  
Hyun Jee Lee ◽  
Hyun June Moon ◽  
Sun Min Kim ◽  
Ki Wan Bong
Keyword(s):  
High Performance ◽  
Hydrogel Microparticles ◽  
Microrna Detection ◽  
Post Synthesis

DNA-templated in situ growth of AgNPs on SWNTs: a new approach for highly sensitive SERS assay of microRNA

2015 ◽  
Vol 51 (30) ◽  
pp. 6552-6555 ◽  
Author(s):  
Jing Zheng ◽  
Junhui Bai ◽  
Qifeng Zhou ◽  
Jishan Li ◽  
Yinhui Li ◽  
...  
Keyword(s):  
In Situ Growth ◽  
Hybrid Nanocomposite ◽  
New Approach ◽  
Microrna Detection ◽  
Highly Sensitive

The SWNT@AgNPs hybrid nanocomposite was employed to achieve microRNA detection.

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