A Dean-flow-coupled interfacial viscoelastic fluid for microparticle separation applied in a cell smear method

The Analyst ◽  
2019 ◽  
Vol 144 (20) ◽  
pp. 5934-5946 ◽  
Author(s):  
Xin Shi ◽  
Liyan Liu ◽  
Wenfeng Cao ◽  
Guorui Zhu ◽  
Wei Tan
Keyword(s):  
Microfluidic Device ◽  
Viscoelastic Fluid ◽  
Cell Separation ◽  
Dean Flow ◽  
A Cell

An interfacial microfluidic device realizing cell separation and washing simultaneously and efficiently.

Microfluidic device for sheathless particle focusing and separation using a viscoelastic fluid

2015 ◽  
Vol 1406 ◽  
pp. 244-250 ◽  
Author(s):  
Jeonghun Nam ◽  
Bumseok Namgung ◽  
Chwee Teck Lim ◽  
Jung-Eun Bae ◽  
Hwa Liang Leo ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Viscoelastic Fluid ◽  
Particle Focusing

scholarly journals Sheathless Dean-flow-coupled elasto-inertial particle focusing and separation in viscoelastic fluid

RSC Advances ◽  
2017 ◽  
Vol 7 (6) ◽  
pp. 3461-3469 ◽  
Author(s):  
Dan Yuan ◽  
Say Hwa Tan ◽  
Qianbin Zhao ◽  
Sheng Yan ◽  
Ronald Sluyter ◽  
...  
Keyword(s):  
Viscoelastic Fluid ◽  
Straight Channel ◽  
Inertial Particle ◽  
Channel Section ◽  
Dean Flow ◽  
Particle Focusing

Sheathless particle focusing and separation in viscoelastic fluid is demonstrated using an integrated ECCA (straight channel section with asymmetrical expansion–contraction cavity arrays) straight channel.

Continuous plasma extraction under viscoelastic fluid in a straight channel with asymmetrical expansion–contraction cavity arrays

Lab on a Chip ◽  
2016 ◽  
Vol 16 (20) ◽  
pp. 3919-3928 ◽  
Author(s):  
Dan Yuan ◽  
Jun Zhang ◽  
Ronald Sluyter ◽  
Qianbin Zhao ◽  
Sheng Yan ◽  
...  
Keyword(s):  
Viscoelastic Fluid ◽  
High Purity ◽  
Inertial Effects ◽  
Straight Channel ◽  
Dean Flow ◽  
Plasma Extraction

By exploiting the Dean-flow-coupled elasto-inertial effects, continuous, sheathless, and high purity plasma extraction under viscoelastic fluid in a straight channel with asymmetrical expansion–contraction cavity arrays (ECCA channel) is demonstrated.

Comparison of Geometries for Diffusion-Based Extraction of Dimethyl Sulphoxide From a Cell Suspension

2008 ◽  
Vol 2 (2) ◽  
Author(s):  
Katie Fleming Glass ◽  
Clara Mata ◽  
Ellen K. Longmire ◽  
Allison Hubel
Keyword(s):  
Microfluidic Device ◽  
Flow Rate ◽  
Channel Length ◽  
Dimethyl Sulphoxide ◽  
Hematopoietic Stem ◽  
Cryoprotective Agents ◽  
Multi Stage ◽  
Flow Geometry ◽  
A Cell ◽  
Flow Configurations

Microfluidics can be used in a variety of medical applications. In this study, a microfluidic device is being developed to remove cryoprotective agents from cells post thaw (1–150ml). Hematopoietic stem cells are typically cryopreserved with Dimethyl sulphoxide (DMSO), which is toxic upon infusion. Conventional methods of removing DMSO results in cells losses of 25–30%. The overall objective of this study is to characterize the influence of flow geometry on extraction of DMSO from a cell stream. For all the flow geometries analyzed, flow rate fraction, Peclet Number, and channel geometry had the greatest influence on extraction of DMSO from the cell stream. The range of flow rate fractions that can achieve the desired removal ranges between 0.10 and 0.30. Similarly, the range of Peclet numbers is 250–2500. Distinct differences in channel length could be observed between the different flow configurations studied. The flow rates and channel geometries studied suggest that clinical volumes of cell suspensions (1–100ml) can be processed using a multi-stage microfluidic device in short periods of time (<1hr).

scholarly journals Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis

Micromachines ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 699
Author(s):  
Rohollah Nasiri ◽  
Amir Shamloo ◽  
Javad Akbari ◽  
Peyton Tebon ◽  
Mehmet R. Dokmeci ◽  
...  
Keyword(s):  
Numerical Simulation ◽  
Angular Velocity ◽  
Microfluidic Device ◽  
Cell Separation ◽  
High Efficiency ◽  
Separation Efficiency ◽  
White Blood Cells ◽  
Cell Lysis ◽  
Target Cells ◽  
Separation Unit

Separation of circulating tumor cells (CTCs) from blood samples and subsequent DNA extraction from these cells play a crucial role in cancer research and drug discovery. Microfluidics is a versatile technology that has been applied to create niche solutions to biomedical applications, such as cell separation and mixing, droplet generation, bioprinting, and organs on a chip. Centrifugal microfluidic biochips created on compact disks show great potential in processing biological samples for point of care diagnostics. This study investigates the design and numerical simulation of an integrated microfluidic device, including a cell separation unit for isolating CTCs from a blood sample and a micromixer unit for cell lysis on a rotating disk platform. For this purpose, an inertial microfluidic device was designed for the separation of target cells by using contraction–expansion microchannel arrays. Additionally, a micromixer was incorporated to mix separated target cells with the cell lysis chemical reagent to dissolve their membranes to facilitate further assays. Our numerical simulation approach was validated for both cell separation and micromixer units and corroborates existing experimental results. In the first compartment of the proposed device (cell separation unit), several simulations were performed at different angular velocities from 500 rpm to 3000 rpm to find the optimum angular velocity for maximum separation efficiency. By using the proposed inertial separation approach, CTCs, were successfully separated from white blood cells (WBCs) with high efficiency (~90%) at an angular velocity of 2000 rpm. Furthermore, a serpentine channel with rectangular obstacles was designed to achieve a highly efficient micromixer unit with high mixing quality (~98%) for isolated CTCs lysis at 2000 rpm.

Characterization of the efficiency of a cell separation process by the extent of elimination of a contaminating cell type

1990 ◽  
Vol 191 (1) ◽  
pp. 144-155 ◽  
Author(s):  
Walter J. Bruyninckx ◽  
Walter H. Vanneste ◽  
Peter J.C. Leijh ◽  
Ralph Van Furth ◽  
Roger E. Vercauteren
Keyword(s):  
Cell Separation ◽  
Separation Process ◽  
Cell Type ◽  
A Cell

Cell separation by an aqueous two-phase system in a microfluidic device

The Analyst ◽  
2009 ◽  
Vol 134 (10) ◽  
pp. 1994 ◽  
Author(s):  
Masatoshi Tsukamoto ◽  
Shu Taira ◽  
Shohei Yamamura ◽  
Yasutaka Morita ◽  
Naoki Nagatani ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Cell Separation ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Deformability and size-based cancer cell separation using an integrated microfluidic device

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7335-7346 ◽  
Author(s):  
Long Pang ◽  
Shaofei Shen ◽  
Chao Ma ◽  
Tongtong Ma ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Cell Size ◽  
Cancer Cell ◽  
Microfluidic Device ◽  
Cell Separation

We present an integrated microfluidic device for cell separation based on the cell size and deformability by combining the microstructure-constricted filtration and pneumatic microvalves.

An inertial microfluidic device for targeted cell separation

2018 ◽  
Vol 44 ◽  
pp. S129
Author(s):  
A. Shamloo ◽  
A. Mashhadian
Keyword(s):  
Microfluidic Device ◽  
Cell Separation
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