Comparison of Geometries for Diffusion-Based Extraction of Dimethyl Sulphoxide From a Cell Suspension

2008 ◽  
Vol 2 (2) ◽  
Author(s):  
Katie Fleming Glass ◽  
Clara Mata ◽  
Ellen K. Longmire ◽  
Allison Hubel
Keyword(s):  
Microfluidic Device ◽  
Flow Rate ◽  
Channel Length ◽  
Dimethyl Sulphoxide ◽  
Hematopoietic Stem ◽  
Cryoprotective Agents ◽  
Multi Stage ◽  
Flow Geometry ◽  
A Cell ◽  
Flow Configurations

Microfluidics can be used in a variety of medical applications. In this study, a microfluidic device is being developed to remove cryoprotective agents from cells post thaw (1–150ml). Hematopoietic stem cells are typically cryopreserved with Dimethyl sulphoxide (DMSO), which is toxic upon infusion. Conventional methods of removing DMSO results in cells losses of 25–30%. The overall objective of this study is to characterize the influence of flow geometry on extraction of DMSO from a cell stream. For all the flow geometries analyzed, flow rate fraction, Peclet Number, and channel geometry had the greatest influence on extraction of DMSO from the cell stream. The range of flow rate fractions that can achieve the desired removal ranges between 0.10 and 0.30. Similarly, the range of Peclet numbers is 250–2500. Distinct differences in channel length could be observed between the different flow configurations studied. The flow rates and channel geometries studied suggest that clinical volumes of cell suspensions (1–100ml) can be processed using a multi-stage microfluidic device in short periods of time (<1hr).

LTCC 3D FLOW FOCALIZATION DEVICE FOR LIQUID-LIQUID PARTIAL SOLVENT EXTRACTION

2016 ◽  
Vol 2016 (CICMT) ◽  
pp. 000111-000117
Author(s):  
Houari Cobas Gomez ◽  
Jéssica Gonçalves da Silva ◽  
Jocasta Mileski Machado ◽  
Bianca Oliveira Agio ◽  
Francisco Jorge Soares de Oliveira ◽  
...  
Keyword(s):  
Solvent Extraction ◽  
Aqueous Phase ◽  
Microfluidic Device ◽  
Flow Rate ◽  
Extraction Efficiency ◽  
Channel Length ◽  
Main Channel ◽  
Process Variables ◽  
3D Flow ◽  
Acetone Concentration

Abstract The present work shows a ceramics microfluidic device for partial solvent extraction scheme. The technology used for device fabrication was Low Temperature Cofired Ceramics (LTCC) which allows us for complex and chemical resistant 3D microfluidic devices. The proposed system aims to partially extract the solvent present in a mixture containing aqueous and organic phases. This scheme uses a 3D flow focalization in order to improve the solvent diffusion into the external aqueous phase. The device is composed by three different parts, the input channels distribution, the main channel and the output channels distribution. The designed input channels distribution ensures a centered 3D focalized solvent stream along the main channel. The focalized solvent mixes with the surrounding water thanks to diffusion. Projected output channels take the central fluid out separately from the surrounding. Thus the device has two different outputs, one for the focalized fluid and another one for the waste fluid, which is the aqueous phase plus solvent. For a device concept proof, acetone and water were used as organic and aqueous phases, respectively. COMSOL Multiphysics was used for device microfluidics and chemical transport simulation. The extraction efficiency was the variable used as indicator for device performance validation. The flow rate ratio between phases, total flow rate, main channel length and focalized stream channel output hydraulic diameter (ODH) were used as process variables for simulation purposes. A factorial experimental planning was used in order to analyze the extraction efficiency taking into account process variables effects. From simulation results it was determined main channel length and ODH as the variables with stronger effect on extraction efficiency. Obtained simulated efficiencies were as high as 80.6%. Considering previous results observations a microfluidic device was fabricated with a main channel length of 21,4 mm and ODH of 214,63 μm. Gas chromatography was used to measured acetone concentration in outputs samples and from here the extraction efficiency. Experimental results were in agreement with simulation, returning extraction efficiencies in the order of 80.8% ± 2.2%.

Continuous separation of particles using a microfluidic device equipped with flow rate control valves

2006 ◽  
Vol 1127 (1-2) ◽  
pp. 214-220 ◽  
Author(s):  
Yuushi Sai ◽  
Masumi Yamada ◽  
Masahiro Yasuda ◽  
Minoru Seki
Keyword(s):  
Microfluidic Device ◽  
Flow Rate ◽  
Rate Control ◽  
Continuous Separation ◽  
Control Valves ◽  
Flow Rate Control

scholarly journals Combination gene therapy for HIV using a conditional suicidal gene with CCR5 knockout

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Tugba Mehmetoglu-Gurbuz ◽  
Rose Yeh ◽  
Himanshu Garg ◽  
Anjali Joshi
Keyword(s):  
Gene Therapy ◽  
Lentiviral Vector ◽  
Suicide Gene ◽  
Vector System ◽  
Tat Protein ◽  
Ccr5 Gene ◽  
Hematopoietic Stem ◽  
Therapy Strategy ◽  
Hiv Tat ◽  
A Cell

Abstract Background Gene therapy approaches using hematopoietic stem cells to generate an HIV resistant immune system have been shown to be successful. The deletion of HIV co-receptor CCR5 remains a viable strategy although co-receptor switching to CXCR4 remains a major pitfall. To overcome this, we designed a dual gene therapy strategy that incorporates a conditional suicide gene and CCR5 knockout (KO) to overcome the limitations of CCR5 KO alone. Methods A two-vector system was designed that included an integrating lentiviral vector that expresses a HIV Tat dependent Thymidine Kinase mutant SR39 (TK-SR39) and GFP reporter gene. The second non-integrating lentiviral (NIL) vector expresses a CCR5gRNA-CRISPR/Cas9 cassette and HIV Tat protein. Results Transduction of cells sequentially with the integrating followed by the NIL vector allows for insertion of the conditional suicide gene, KO of CCR5 and transient expression of GFP to enrich the modified cells. We used this strategy to modify TZM cells and generate a cell line that was resistant to CCR5 tropic viruses while permitting infection of CXCR4 tropic viruses which could be controlled via treatment with Ganciclovir. Conclusions Our study demonstrates proof of principle that a combination gene therapy for HIV is a viable strategy and can overcome the limitation of editing CCR5 gene alone.

Non-contact-based determination of membrane permeability to water and dimethyl sulfoxide of cells virtually trapped in a self-induced micro-vortex

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Hsiu-Yang Tseng ◽  
Chiu-Jen Chen ◽  
Zong-Lin Wu ◽  
Yong-Ming Ye ◽  
Guo-Zhen Huang
Keyword(s):  
Cell Membrane ◽  
Dimethyl Sulfoxide ◽  
Membrane Permeability ◽  
Cell Membrane Permeability ◽  
Cell Type ◽  
Cryoprotective Agents ◽  
A Cell

Cell-membrane permeability to water (Lp) and cryoprotective agents (Ps) of a cell type is a crucial cellular information for achieving optimal cryopreservation in the biobanking industry. In this work, a...

Physical basis of the dependence of blood viscosity on tube radius

1960 ◽  
Vol 198 (6) ◽  
pp. 1193-1200 ◽  
Author(s):  
Robert H. Haynes
Keyword(s):  
Flow Rate ◽  
Blood Viscosity ◽  
Apparent Viscosity ◽  
Hind Limb ◽  
Marginal Zone ◽  
Tube Wall ◽  
Effective Diameter ◽  
Tube Radius ◽  
Vascular Bed ◽  
A Cell

Two theories are applied to measurements of the decrease in apparent viscosity of blood in narrow tubes (Fahraeus-Lindqvist effect). First, the effect may be attributed to the presence of unsheared laminae in the fluid (sigma phenomenon), and it was found that the thickness of such laminae must vary between 3.5 µ at 10% hematocrit and 34 µ at 80%. Alternatively, the effect may be caused by a cell-free marginal zone adjacent to the tube wall, which would have to be 6 µ thick at 10% hematocrit and 1.5 µ at 80%. There is a slight suggestion in the data that the effect may be reversed as the flow rate approaches zero (i.e. the apparent viscosity rises in small tubes). Finally, a method is proposed for calculating the effective diameter of a vascular bed, and it was found to be 55 µ for a dog's hind limb.

scholarly journals Annexin II expressed by osteoblasts and endothelial cells regulates stem cell adhesion, homing, and engraftment following transplantation

Blood ◽  
2007 ◽  
Vol 110 (1) ◽  
pp. 82-90 ◽  
Author(s):  
Younghun Jung ◽  
Jingcheng Wang ◽  
Junhui Song ◽  
Yusuke Shiozawa ◽  
Jianhua Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Annexin Ii ◽  
Bone Marrow Niche ◽  
Peptide Fragments ◽  
Hematopoietic Stem ◽  
Marrow Cavity ◽  
Endosteal Niche ◽  
A Cell ◽  
Adhesive Interactions

Differentiation of hematopoietic stem cells (HSCs) after birth is largely restricted to the bone marrow cavity, where HSCs are associated closely with osteoblasts (OBs). How OBs localize HSCs to the endosteal niche remains unclear. To explore adhesive interactions between HSCs and OBs, a cell blot analysis was used that revealed 2 major bands that corresponded to monomers and multimers of annexin II (Anxa2). Immunohistochemistry revealed that OBs and marrow endothelial cells express Anxa2 at high levels. Function-blocking studies confirmed that Anxa2 mediates HSC adhesion mainly via the N-terminal portion of the Anxa2 peptide. Adhesion of HSCs to OBs derived from Anxa2-deficient animals (Anxa2−/−) was significantly impaired compared with OBs obtained from wild-type animals (Anxa2+/+). Moreover, fewer HSCs were found in the marrow of Anxa2−/− versus Anxa2+/+ animals. Short-term lodging, engraftment, and survival of irradiated mice with whole marrow cells were substantially inhibited by N-terminal peptide fragments of Anxa2 or anti-Anxa2 antibodies. Similar findings were noted in long-term competitive repopulation studies. Collectively, these findings reveal that Anxa2 regulates HSC homing and binding to the bone marrow microenvironment and suggest that Anxa2 is crucial for determining the bone marrow niche of HSCs.

scholarly journals Integration of a Solar Parabolic Dish Collector with a Small-Scale Multi-Stage Flash Desalination Unit: Experimental Evaluation, Exergy and Economic Analyses

2021 ◽  
Vol 13 (20) ◽  
pp. 11295
Author(s):  
Ali Babaeebazaz ◽  
Shiva Gorjian ◽  
Majid Amidpour
Keyword(s):  
Solar Radiation ◽  
Flow Rate ◽  
Quality Parameters ◽  
Small Scale ◽  
Conical Cavity ◽  
Multi Stage ◽  
Circulation Circuit ◽  
Parabolic Dish ◽  
Economic Analyses ◽  
The Cost

In this study, a small-scale two-stage multi-stage flash (MSF) desalination unit equipped with a vacuum pump and a solar parabolic collector (PDC) with a conical cavity receiver were integrated. To eliminate the need for heat exchangers, a water circulation circuit was designed in a way that the saline feedwater could directly flow through the receiver of the PDC. The system’s performance was examined during six days in July 2020, from 10:00 a.m. to 3:00 p.m., under two distinct scenarios of the MSF desalination operation under the vacuum (−10 kPa) and atmospheric pressure by considering three saline feedwater water flow rates of 0.7, 1 and 1.3 L/min. Furthermore, the performance of the solar PDC-MSF desalination plant was evaluated by conducting energy and exergy analyses. The results indicated that the intensity of solar radiation, which directly affects the top brine temperature (TBT), and the values of the saline feedwater flow rate have the most impact on productivity. The maximum productivity of 3.22 L per 5 h in a day was obtained when the temperature and saline feedwater flow rate were 94.25 °C (at the maximum solar radiation of 1015.3 W/m2) and 0.7 L/min, respectively, and the MSF was under vacuum pressure. Additionally, it was found that increasing the feedwater flow rate from 0.7 to 1.3 L/min reduces distillate production by 76.4% while applying the vacuum improves the productivity by about 34% at feedwater flow rate of 0.7 L/min. The exergy efficiency of the MSF unit was obtained as 0.07% with the highest share of exergy destruction in stages. The quality parameters of the produced distillate including pH, TDS, EC and DO were measured, ensuring they lie within the standard range for drinking water. Moreover, the cost of freshwater produced by the MSF plant varied from 37 US$/m3 to 1.5 US$/m3 when the treatment capacity increased to 8000 L/day.

scholarly journals Normal cycling patterns of hematopoietic stem cell subpopulations: an assay using long-term in vivo BrdU infusion

Blood ◽  
1985 ◽  
Vol 66 (6) ◽  
pp. 1460-1462 ◽  
Author(s):  
ME Pietrzyk ◽  
GV Priestley ◽  
NS Wolf
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Improved Method ◽  
Hematopoietic Stem ◽  
Infusion Study ◽  
A Cell ◽  
Cell Subpopulations ◽  
Over Time

It was found in a long-term bromodeoxyuridine (BrdU) infusion study that two or more different subpopulations of bone marrow stem cells exist in mice. One of these subpopulations appears to be noncycling and forms approximately 10% of eight-day CFU-S. Another one, a subpopulation of slowly cycling bone marrow cells, is represented as 14- day CFU-S. The 14-day CFU-S have a regular increment in the percentage of the subpopulation entering the cycle over time, with a cell generation half-time of 21 days. The cycling status in these experiments was ascertained by in vivo continuous long-term BrdU infusion. An improved method is presented for long-term BrdU infusion with UV killing of cycled cells.

LTCC 3D MICROMIXERS FOR NON-MISCIBLE FLUIDS MICROEMULSION GENERATION

2016 ◽  
Vol 2016 (CICMT) ◽  
pp. 000096-000102
Author(s):  
Houari Cobas Gomez ◽  
Bianca Oliveira Agio ◽  
Jéssica Gonçalves da Silva ◽  
Natalia Neto Pereira Cerize ◽  
Adriano Marim de Oliveira ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Flow Rate ◽  
Device Fabrication ◽  
Chaotic Advection ◽  
Drop Diameter ◽  
Production Volume ◽  
Total Flow ◽  
Total Flow Rate ◽  
Miscible Fluids ◽  
Working Region

Abstract The present work shows a ceramics microfluidic device for non-miscible fluids microemulsion generation using 3D serpentine micromixers. The technology used for device fabrication was Low Temperature Cofired Ceramics (LTCC) which allows us for complex, high temperature and pressure resistant 3D microfluidic devices. The proposed device aims to obtain microemulsion with controlled drop size, low dispersion index and high production volumes using Top-Down approach. Previous simulation work had showed 3D serpentine as one of the best structures for rapid mixing due the chaotic advection generated on every 90 deg direction change. This effect, when mixing two fluids as oil and water leads to streamlines pinching-off making possible drop generation. We have used this effect on our device. For the experimental section, it was fabricated a 3D serpentine mixer microfluidic device with working region suitable for variable total flow rate. For certain value of total flow rate, the microemulsion showed higher drop diameter and polydispersity values. In this region, no control could be done in order to obtain the same drop value with the same process parameters. Inside the working region drop diameter values repeatability was obtained. In this region our experimental results had showed a relation between drop diameter and total flow rate. As a total flow rate increase the drop diameter decrease due to a stronger chaotic advection effect. In the other hand, the polydispersity index also decreases. Microemulsions with average size lower than few micrometer or submicron were obtained. When compared with other reported devices, our device presented a production volume in the range of tens of ml/s for the same output microemulsion size.

scholarly journals Learning The Fluid/Flow Properties Using Microfluidics

2019 ◽  
Vol 215 ◽  
pp. 10002
Author(s):  
Pooria Hadikhani ◽  
Navid Borhani ◽  
S. Mohammad H. Hashemi ◽  
Demetri Psaltis
Keyword(s):  
Neural Networks ◽  
Fluid Flow ◽  
Microfluidic Device ◽  
Flow Rate ◽  
Deep Neural Networks ◽  
Good Accuracy ◽  
Flow Properties ◽  
Flow Rates

Deep neural networks (DNN) are employed to measure the flow rate and the concentration of the liquid using the images of the droplets in a microfluidic device. The trained networks are able to measure flow rates and concentrations with good accuracy.

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