Silver ion-stabilized DNA triplexes for completely enzyme-free and sensitive fluorescence detection of transcription factors via catalytic hairpin assembly amplification

2019 ◽  
Vol 7 (5) ◽  
pp. 763-767 ◽  
Author(s):  
Yao Qin ◽  
Daxiu Li ◽  
Ruo Yuan ◽  
Yun Xiang
Keyword(s):  
Transcription Factors ◽  
Fluorescence Detection ◽  
Silver Ion ◽  
Dna Triplexes ◽  
Catalytic Hairpin Assembly ◽  
Dna Triplex

A new silver ion-stabilized DNA triplex enables enzyme-free and amplified sensitive fluorescence detection of transcription factors.

scholarly journals DNA triplex structure, thermodynamics, and destabilisation: insight from molecular simulations

2018 ◽  
Vol 20 (20) ◽  
pp. 14013-14023 ◽  
Author(s):  
Belinda J. Boehm ◽  
Charles Whidborne ◽  
Alexander L. Button ◽  
Tara L. Pukala ◽  
David M. Huang
Keyword(s):  
Molecular Dynamics ◽  
Neurodegenerative Disease ◽  
Molecular Dynamics Simulations ◽  
Small Molecule ◽  
Molecular Simulations ◽  
Friedreich’S Ataxia ◽  
Dna Triplexes ◽  
Dna Triplex ◽  
Triplex Structure ◽  
Dynamics Simulations

Molecular dynamics simulations are used to elucidate the structure and thermodynamics of DNA triplexes associated with the neurodegenerative disease Friedreich's ataxia (FRDA), as well as complexes of these triplexes with the small molecule netropsin, which is known to destabilise triplexes.

Silver Ion Unusually Stabilizes the Structure of a Parallel-Motif DNA Triplex

2009 ◽  
Vol 131 (11) ◽  
pp. 3826-3827 ◽  
Author(s):  
Toshihiro Ihara ◽  
Tatsuaki Ishii ◽  
Norie Araki ◽  
Anthony W. Wilson ◽  
Akinori Jyo
Keyword(s):  
Silver Ion ◽  
Dna Triplex

Ultrasensitive fluorescence detection of transcription factors based on kisscomplex formation and the T7 RNA polymerase amplification method

2017 ◽  
Vol 53 (43) ◽  
pp. 5846-5849 ◽  
Author(s):  
Kai Zhang ◽  
Ke Wang ◽  
Xue Zhu ◽  
Minhao Xie
Keyword(s):  
Transcription Factors ◽  
Rna Polymerase ◽  
Fluorescence Detection ◽  
Detection Limits ◽  
High Sensitivity ◽  
Fluorescence Assay ◽  
T7 Rna Polymerase ◽  
Protein Fluorescence ◽  
Amplification Method

Herein, we report a kisscomplex based protein fluorescence assay (KPFA) method, which employed the formation of a kisscomplex and the T7 RNA polymerase amplification method, for the assay of transcription factors with high sensitivity. The detection limits of MITF and NF-κB p65 are 0.23 pM and 0.496 pM, respectively.

Enzyme-free signal amplified Au nanoparticle fluorescence detection of thrombin via target-triggered catalytic hairpin assembly

2021 ◽  
Vol 160 ◽  
pp. 105649
Author(s):  
Terefe Tafese Bezuneh ◽  
Tadesse Haile Fereja ◽  
Shimeles Addisu Kitte ◽  
Haijuan Li ◽  
Yongdong Jin
Keyword(s):  
Fluorescence Detection ◽  
Au Nanoparticle ◽  
Catalytic Hairpin Assembly

Silver-ion-mediated Mg2+-dependent DNAzyme activity for amplified fluorescence detection of cysteine

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 1982-1987 ◽  
Author(s):  
Xu-Hua Zhao ◽  
Li-Zhuan Zhang ◽  
Su-Ya Zhao ◽  
Xiao-Hua Cui ◽  
Liang Gong ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Silver Ion ◽  
Fluorescent Biosensor

Schematic illustration of a DNAzyme-based fluorescent biosensor for amplified Cys detection.

scholarly journals Interaction of silver ion with CG.C+ base triplets in DNA triplex

2009 ◽  
Vol 53 (1) ◽  
pp. 19-20 ◽  
Author(s):  
T. Ihara ◽  
T. Ishii ◽  
A. Jyo
Keyword(s):  
Silver Ion ◽  
Dna Triplex

Amplified probing of protein/DNA interactions for sensitive fluorescence detection of transcription factors

2018 ◽  
Vol 6 (37) ◽  
pp. 6002-6007 ◽  
Author(s):  
Jin Li ◽  
Jinshan Tang ◽  
Bingying Jiang ◽  
Yun Xiang ◽  
Ruo Yuan
Keyword(s):  
Transcription Factors ◽  
Fluorescence Detection ◽  
Metal Ion ◽  
Dna Interactions ◽  
Protection Strategy ◽  
Protein Dna Interactions ◽  
Enzyme Protection

Coupling the enzyme protection strategy with metal-ion dependent DNAzyme amplification leads to sensitive monitoring of protein/DNA interactions.

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