Ultrasensitive fluorescence detection of transcription factors based on kisscomplex formation and the T7 RNA polymerase amplification method

2017 ◽  
Vol 53 (43) ◽  
pp. 5846-5849 ◽  
Author(s):  
Kai Zhang ◽  
Ke Wang ◽  
Xue Zhu ◽  
Minhao Xie
Keyword(s):  
Transcription Factors ◽  
Rna Polymerase ◽  
Fluorescence Detection ◽  
Detection Limits ◽  
High Sensitivity ◽  
Fluorescence Assay ◽  
T7 Rna Polymerase ◽  
Protein Fluorescence ◽  
Amplification Method

Herein, we report a kisscomplex based protein fluorescence assay (KPFA) method, which employed the formation of a kisscomplex and the T7 RNA polymerase amplification method, for the assay of transcription factors with high sensitivity. The detection limits of MITF and NF-κB p65 are 0.23 pM and 0.496 pM, respectively.

scholarly journals Stringent control in Escherichia coli applies also to transcription by T7 RNA polymerase.

1987 ◽  
Vol 262 (9) ◽  
pp. 3940-3943
Author(s):  
M. Yamagishi ◽  
J.R. Cole ◽  
M. Nomura ◽  
F.W. Studier ◽  
J.J. Dunn
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
T7 Rna Polymerase ◽  
Stringent Control
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