dna triplexes
Recently Published Documents


TOTAL DOCUMENTS

93
(FIVE YEARS 2)

H-INDEX

23
(FIVE YEARS 0)

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Shiliang He ◽  
Zhilei Ge ◽  
Xiaolei Zuo ◽  
Chunhai Fan ◽  
Xiuhai Mao

AbstractDNA nanostructures are among the most fascinating self-assembled nanostructures in diverse areas of science and technology, because of their nanoscale precision in biomolecule and nanoparticle organization. The implementation of dynamic and spatial regulation in structural morphology and hierarchical assembly upon specific external stimuli will greatly expand their applications in biocomputation, clinical diagnosis, and cancer therapy. Recently, noncanonical nucleic acids, particularly DNA triplexes, i-motifs, and G-quadruplexes, have become powerful tools for biosensing and mechanical switching. Developments in incorporating stimuli-responsive noncanonical nucleic acids into DNA nanostructures provide a promising approach to regulating the spatial organization and hierarchical assembly of DNA nanostructures. In this review, we briefly introduce recent progress in constructing DNA nanostructures with dynamic regulation of the structural transformation and programmable assembly pathways at the nanometer scale by noncanonical nucleic acids and discuss their potential applications and challenges.


2021 ◽  
Vol 17 ◽  
pp. 749-761
Author(s):  
Yongdong Su ◽  
Maitsetseg Bayarjargal ◽  
Tracy K Hale ◽  
Vyacheslav V Filichev

Two phosphate modifications were introduced into the DNA backbone using the Staudinger reaction between the 3’,5’-dinucleoside β-cyanoethyl phosphite triester formed during DNA synthesis and sulfonyl azides, 4-(azidosulfonyl)-N,N,N-trimethylbutan-1-aminium iodide (N+ azide) or p-toluenesulfonyl (tosyl or Ts) azide, to provide either a zwitterionic phosphoramidate with N+ modification or a negatively charged phosphoramidate for Ts modification in the DNA sequence. The incorporation of these N+ and Ts modifications led to the formation of thermally stable parallel DNA triplexes, regardless of the number of modifications incorporated into the oligodeoxynucleotides (ONs). For both N+ and Ts-modified ONs, the antiparallel duplexes formed with complementary RNA were more stable than those formed with complementary DNA (except for ONs with modification in the middle of the sequence). Additionally, the incorporation of N+ modifications led to the formation of duplexes with a thermal stability that was less dependent on the ionic strength than native DNA duplexes. The thermodynamic analysis of the melting curves revealed that it is the reduction in unfavourable entropy, despite the decrease in favourable enthalpy, which is responsible for the stabilisation of duplexes with N+ modification. N+ONs also demonstrated greater resistance to nuclease digestion by snake venom phosphodiesterase I than the corresponding Ts-ONs. Cell uptake studies showed that Ts-ONs can enter the nucleus of mouse fibroblast NIH3T3 cells without any transfection reagent, whereas, N+ONs remain concentrated in vesicles within the cytoplasm. These results indicate that both N+ and Ts-modified ONs are promising for various in vivo applications.


2020 ◽  
Author(s):  
Yongdong Su ◽  
Maitsetseg Bayarjargal ◽  
Tracy K Hale ◽  
Vyacheslav V Filichev

Two phosphate modifications were introduced into the DNA backbone using Staudinger reaction between the 3’,5’-dinucleoside β-cyanoethyl phosphite triester formed during DNA synthesis and the sulfonyl azides, 4-(azidosulfonyl)-N,N,N-trimethylbutan-1-aminium iodide (N+ azide) or p-toluenesulfonyl (tosyl or Ts) azide, to provide either a zwitterionic phosphoramidate with N+ modification or a negatively charged phosphoramidate for Ts- modification in the DNA sequence. Incorporation of these N+ and Ts- modifications led to the formation of thermally stable parallel DNA triplexes, regardless of the number of modifications incorporated into the oligodeoxynucleotides (ONs). For both N+ and Ts- modified ONs, the antiparallel duplexes formed with complementary RNA were more stable than those formed with complementary DNA (except for ONs where the modification is in the middle of the sequence). Incorporation of N+ modifications led to the formation of duplexes whose thermal stability was less dependent on ionic strength than native DNA duplexes. Thermodynamic analysis of melting curves revealed that it is a reduction in unfavourable entropy, despite the decrease in favourable enthalpy, which is responsible for the stabilisation of duplexes with N+ modification. N+ ONs also demonstrated greater resistance to nuclease digestion by snake venom phosphodiesterase I than the corresponding Ts-ONs. Cell permeability studies showed that Ts- ONs diffuse into the nucleus of mouse fibroblast NIH3T3 cells without the need for transfection reagents. In contrast, N+ ONs were concentrated in vesicles within the cytoplasm. These results indicate that both N+ and Ts- modified ONs are promising for various in vivo applications.


2020 ◽  
Vol 48 (17) ◽  
pp. 9899-9917
Author(s):  
Jiahui Zhang ◽  
Ashkan Fakharzadeh ◽  
Feng Pan ◽  
Christopher Roland ◽  
Celeste Sagui

Abstract Expansion of the GAA/TTC repeats in the first intron of the FXN gene causes Friedreich’s ataxia. Non-canonical structures are linked to this expansion. DNA triplexes and R-loops are believed to arrest transcription, which results in frataxin deficiency and eventual neurodegeneration. We present a systematic in silico characterization of the possible DNA triplexes that could be assembled with GAA and TTC strands; the two hybrid duplexes [r(GAA):d(TTC) and d(GAA):r(UUC)] in an R-loop; and three hybrid triplexes that could form during bidirectional transcription when the non-template DNA strand bonds with the hybrid duplex (collapsed R-loops, where the two DNA strands remain antiparallel). For both Y·R:Y and R·R:Y DNA triplexes, the parallel third strand orientation is more stable; both parallel and antiparallel protonated d(GA+A)·d(GAA):d(TTC) triplexes are stable. Apparent contradictions in the literature about the R·R:Y triplex stability is probably due to lack of molecular resolution, since shifting the third strand by a single nucleotide alters the stability ranking. In the collapsed R-loops, antiparallel d(TTC+)·d(GAA):r(UUC) is unstable, while parallel d(GAA)·r(GAA):d(TTC) and d(GA+A)·r(GAA):d(TTC) are stable. In addition to providing new structural perspectives for specific therapeutic aims, our results contribute to a systematic structural basis for the emerging field of quantitative R-loop biology.


2020 ◽  
Vol 26 (25) ◽  
pp. 5676-5684
Author(s):  
Chenguang Lou ◽  
Josephine Tuborg Boesen ◽  
Niels Johan Christensen ◽  
Kasper K. Sørensen ◽  
Peter W. Thulstrup ◽  
...  

2019 ◽  
Vol 47 (15) ◽  
pp. 8084-8095 ◽  
Author(s):  
Haiying Liu ◽  
Yiding Yang ◽  
Yuanlong Ge ◽  
Juanhong Liu ◽  
Yong Zhao

Abstract TERC is an RNA component of telomerase. However, TERC is also ubiquitously expressed in most human terminally differentiated cells, which don’t have telomerase activity. The function of TERC in these cells is largely unknown. Here, we report that TERC enhances the expression and secretion of inflammatory cytokines by stimulating NK-κB pathway in a telomerase-independent manner. The ectopic expression of TERC in telomerase-negative cells alters the expression of 431 genes with high enrichment of those involved in cellular immunity. We perform genome-wide screening using a previously identified ‘binding motif’ of TERC and identify 14 genes that are transcriptionally regulated by TERC. Among them, four genes (LIN37, TPRG1L, TYROBP and USP16) are demonstrated to stimulate the activation of NK-κB pathway. Mechanistically, TERC associates with the promoter of these genes through forming RNA–DNA triplexes, thereby enhancing their transcription. In vivo, expression levels of TERC and TERC target genes (TYROBP, TPRG1L and USP16) are upregulated in patients with inflammation-related diseases such as type II diabetes and multiple sclerosis. Collectively, these results reveal an unknown function of TERC on stimulating inflammatory response and highlight a new mechanism by which TERC modulates gene transcription. TERC may be a new target for the development of anti-inflammation therapeutics.


2019 ◽  
Vol 7 (5) ◽  
pp. 763-767 ◽  
Author(s):  
Yao Qin ◽  
Daxiu Li ◽  
Ruo Yuan ◽  
Yun Xiang

A new silver ion-stabilized DNA triplex enables enzyme-free and amplified sensitive fluorescence detection of transcription factors.


Nanoscale ◽  
2019 ◽  
Vol 11 (39) ◽  
pp. 18026-18030 ◽  
Author(s):  
Shuo Yang ◽  
Wenyan Liu ◽  
Risheng Wang

We demonstrate the pH-regulated, multistep self-assembly of DNA nanostructures by employing DNA triplexes as dynamic linkers in a stepwise, selective, and reversible fashion.


2018 ◽  
Vol 58 (4) ◽  
pp. 773-783 ◽  
Author(s):  
Yossa Dwi Hartono ◽  
You Xu ◽  
Andrey Karshikoff ◽  
Lennart Nilsson ◽  
Alessandra Villa

2018 ◽  
Vol 20 (20) ◽  
pp. 14013-14023 ◽  
Author(s):  
Belinda J. Boehm ◽  
Charles Whidborne ◽  
Alexander L. Button ◽  
Tara L. Pukala ◽  
David M. Huang

Molecular dynamics simulations are used to elucidate the structure and thermodynamics of DNA triplexes associated with the neurodegenerative disease Friedreich's ataxia (FRDA), as well as complexes of these triplexes with the small molecule netropsin, which is known to destabilise triplexes.


Sign in / Sign up

Export Citation Format

Share Document