Promoting intracellular delivery of sub-25 nm nanoparticles via defined levels of compression

Nanoscale ◽  
2018 ◽  
Vol 10 (31) ◽  
pp. 15090-15102 ◽  
Author(s):  
Hongrong Yang ◽  
Yifei Yao ◽  
Huize Li ◽  
Lok Wai Cola Ho ◽  
Bohan Yin ◽  
...  
Keyword(s):  
Uniaxial Compression ◽  
Cellular Uptake ◽  
Intracellular Delivery ◽  
Set Up

An experimental set-up for evaluating the cellular uptake of sub-25 nm nanoparticles under defined levels of uniaxial compression is presented.

Probing Cellular Outcomes Using Heterobivalent Constructs

2019 ◽  
Author(s):  
Rohit Bhadoria ◽  
Kefeng Ping ◽  
Christer Lohk ◽  
Ivar Järving ◽  
Pavel Starkov
Keyword(s):  
Cell Viability ◽  
Small Molecules ◽  
Cellular Uptake ◽  
Kinase Inhibitors ◽  
Rho Kinase ◽  
Intracellular Delivery ◽  
Rho Kinase Inhibitors

<div> <div> <div> <p>Conjugation techniques are central to improving intracellular delivery of bioactive small molecules. However, tracking and assessing the overall biological outcome of these constructs remains poorly understood. We addressed this issue by having developed a focused library of heterobivalent constructs based on Rho kinase inhibitors to probe various scenarios. By comparing induction of a phenotype of interest vs. cell viability vs. cellular uptake, we demonstrate that such conjugates indeed lead to divergent cellular outcomes. </p> </div> </div> </div>

Efficient cellular uptake of click nucleic acid modified proteins

2020 ◽  
Vol 56 (35) ◽  
pp. 4820-4823 ◽  
Author(s):  
Albert Harguindey ◽  
Heidi R. Culver ◽  
Jasmine Sinha ◽  
Christopher N. Bowman ◽  
Jennifer N. Cha
Keyword(s):  
Nucleic Acid ◽  
Cellular Uptake ◽  
Intracellular Delivery ◽  
Modified Proteins

Efficient intracellular delivery of biomacromolecules such as proteins continues to remain a challenge despite its potential for medicine.

Synthesis, Cellular Uptake, and Cytotoxicity of a Thermally Responsive Nanocapsule

2009 ◽  
Author(s):  
Wujie Zhang ◽  
Jianhua Rong ◽  
Qian Wang ◽  
Xiaoming He
Keyword(s):  
Cellular Uptake ◽  
Blood Cells ◽  
Mammalian Cells ◽  
Polymeric Nanoparticles ◽  
Intracellular Delivery ◽  
Sustained Delivery ◽  
Hydrogel Nanoparticles ◽  
Ambient Temperatures ◽  
Thermally Responsive ◽  
Therapeutic Drugs

Recently, polymeric nanoparticles have attracted tremendous interests as a useful tool to encapsulate therapeutic drugs, genes, and proteins for their controlled and sustained delivery. Among them, polymeric hydrogel nanoparticles with thermal and/or pH responsiveness have attracted particular attention [1]. Trehalose, a non-reducing disaccharide of glucose, has been demonstrated to be a potent, nontoxic bioprotectant for stabilizing lipids, proteins, viruses, and blood cells at cryogenic and particularly, ambient temperatures (i.e., cryo and lyopreservation) [2]. However, intracellular delivery of trehalose into small eukaryotic mammalian cells in a large quantity for biostabilization purpose has not been very successful so far [2]. In this study, a thermally responsive polymeric nanocapsule was synthesized and characterized with the aim to encapsulate trehalose for its intracellular delivery.

Intracellular Delivery of Etoposide Loaded Biodegradable Nanoparticles: Cytotoxicity and Cellular Uptake Studies

2011 ◽  
Vol 11 (8) ◽  
pp. 6657-6667 ◽  
Author(s):  
Khushwant S. Yadav ◽  
Sheeba Jacob ◽  
Geetanjali Sachdeva ◽  
Krutika K. Sawant
Keyword(s):  
Cellular Uptake ◽  
Intracellular Delivery ◽  
Biodegradable Nanoparticles ◽  
Uptake Studies

Stearylated cycloarginine nanosystems for intracellular delivery – simulations, formulation and proof of concept

RSC Advances ◽  
2016 ◽  
Vol 6 (114) ◽  
pp. 113538-113550 ◽  
Author(s):  
V. Dhawan ◽  
A. Magarkar ◽  
G. Joshi ◽  
D. Makhija ◽  
A. Jain ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
In Silico ◽  
Intracellular Delivery ◽  
Proof Of Concept ◽  
Reduced Toxicity

Novel cationic agent liposomes performed better in silico translating in higher cellular uptake with reduced toxicity.

scholarly journals Attoliter protein nanogels from droplet nanofluidics for intracellular delivery

2020 ◽  
Vol 6 (6) ◽  
pp. eaay7952 ◽  
Author(s):  
Zenon Toprakcioglu ◽  
Pavan Kumar Challa ◽  
David B. Morse ◽  
Tuomas Knowles
Keyword(s):  
Tissue Engineering ◽  
Cellular Uptake ◽  
Biomedical Applications ◽  
Cell Encapsulation ◽  
Intracellular Delivery ◽  
Natural Protein ◽  
Scale Size ◽  
Oil Emulsions ◽  
Micrometer Scale ◽  
Potential Use

Microscale hydrogels consisting of macromolecular networks in aqueous continuous phases have received increasing attention because of their potential use in tissue engineering, cell encapsulation and for the storage and release of cargo molecules. However, for applications targeting intracellular delivery, their micrometer-scale size is unsuitable for effective cellular uptake. Nanoscale analogs of such materials are thus required for this key area. Here, we describe a microfluidics/nanofluidics-based strategy for generating monodisperse nanosized water-in-oil emulsions with controllable sizes ranging from 2500 ± 110 nm down to 51 ± 6 nm. We demonstrate that these nanoemulsions can act as templates to form protein nanogels stabilized by supramolecular fibrils from three different proteins. We further show that these nanoparticles have the ability to penetrate mammalian cell membranes and deliver intracellular cargo. Due to their biocompatibility and lack of toxicity, natural protein-based nanoparticles present advantageous characteristics as vehicles for cargo molecules in the context of pharmaceutical and biomedical applications.

scholarly journals A Creep Damage Constitutive Model for a Rock Mass with Nonpersistent Joints under Uniaxial Compression

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Shuran Lv ◽  
Wanqing Wang ◽  
Hongyan Liu
Keyword(s):  
Rock Mass ◽  
Constitutive Model ◽  
Mechanical Behavior ◽  
Uniaxial Compression ◽  
Damage Model ◽  
Creep Damage ◽  
Model Parameters ◽  
Damage Constitutive Model ◽  
Set Up ◽  
Creep Damage Model

As part of the rock mass, both the mesoscopic and macroscopic flaws will affect the creep mechanical behavior of the rock mass with nonpersistent joints. This study focuses on this kind of rock mass and establishes a creep damage model to account for the effect of the joint on its creep mechanical behavior. First, on basis of analyzing the rock element creep mechanism and the typical creep deformation curve, a new creep damage constitutive model for the rock element is set up by introducing the damage theory and Kachanov damage evolution law into the classic creep constitutive model such as J body model. Second, the determination method of the proposed model parameters is studied in detail. Third, the calculation method of the macroscopic damage caused by the joint proposed by others is introduced which can consider the joint geometry, strength, and deformation parameters at the same time. Finally, the creep damage model for the rock mass with nonpersistent joints under uniaxial compression is proposed. The calculation examples indicate that it can present the effect of the joint on the rock mass creep mechanical behavior.

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