Efficient cellular uptake of click nucleic acid modified proteins

2020 ◽  
Vol 56 (35) ◽  
pp. 4820-4823 ◽  
Author(s):  
Albert Harguindey ◽  
Heidi R. Culver ◽  
Jasmine Sinha ◽  
Christopher N. Bowman ◽  
Jennifer N. Cha
Keyword(s):  
Nucleic Acid ◽  
Cellular Uptake ◽  
Intracellular Delivery ◽  
Modified Proteins

Efficient intracellular delivery of biomacromolecules such as proteins continues to remain a challenge despite its potential for medicine.

Probing Cellular Outcomes Using Heterobivalent Constructs

2019 ◽  
Author(s):  
Rohit Bhadoria ◽  
Kefeng Ping ◽  
Christer Lohk ◽  
Ivar Järving ◽  
Pavel Starkov
Keyword(s):  
Cell Viability ◽  
Small Molecules ◽  
Cellular Uptake ◽  
Kinase Inhibitors ◽  
Rho Kinase ◽  
Intracellular Delivery ◽  
Rho Kinase Inhibitors

<div> <div> <div> <p>Conjugation techniques are central to improving intracellular delivery of bioactive small molecules. However, tracking and assessing the overall biological outcome of these constructs remains poorly understood. We addressed this issue by having developed a focused library of heterobivalent constructs based on Rho kinase inhibitors to probe various scenarios. By comparing induction of a phenotype of interest vs. cell viability vs. cellular uptake, we demonstrate that such conjugates indeed lead to divergent cellular outcomes. </p> </div> </div> </div>

Peptide-nucleic acid nanostructures for transfection

2012 ◽  
Vol 3 (3) ◽  
pp. 283-293 ◽  
Author(s):  
Burkhard Bechinger
Keyword(s):  
Nucleic Acid ◽  
Physicochemical Properties ◽  
Cellular Uptake ◽  
Peptide Nucleic Acid ◽  
Endosomal Escape ◽  
Medical Applications ◽  
Specific Cell ◽  
Charge Composition ◽  
Cell Surfaces ◽  
Intracellular Targeting

AbstractTo use nucleic acids in biomedical research and medical applications, these highly hydrophilic macromolecules have to be transported through the organism, targeted to specific cell surfaces, and have to cross cellular barriers. To this end, nanosized transfection complexes have been designed and several of them have been successfully tested. Here, the different steps of the transfection process and the particular optimization protocols are reviewed, including the physicochemical properties of such vectors (size, charge, composition), protection in serum, cellular uptake, endosomal escape, and intracellular targeting. The transfection process has been subdivided into separate steps and here special emphasis is given to peptides that have been designed to optimize these steps individually. Finally, complex devices encompassing a multitude of beneficial functionalities for transfection have been developed.

scholarly journals Spontaneous cellular uptake of exogenous messenger RNA in vivo is nucleic acid-specific, saturable and ion dependent

Gene Therapy ◽  
2007 ◽  
Vol 14 (15) ◽  
pp. 1175-1180 ◽  
Author(s):  
J Probst ◽  
B Weide ◽  
B Scheel ◽  
B J Pichler ◽  
I Hoerr ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Cellular Uptake ◽  
Messenger Rna

scholarly journals In Situ Loading and Delivery of Short Single- and Double-Stranded DNA by Supramolecular Organic Frameworks

CCS Chemistry ◽  
2019 ◽  
Vol 1 (2) ◽  
pp. 156-165 ◽  
Author(s):  
Bo Yang ◽  
Xiao-Dan Zhang ◽  
Jian Li ◽  
Jia Tian ◽  
Yi-Peng Wu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Sequences ◽  
Laser Scanning ◽  
High Stability ◽  
Intracellular Delivery ◽  
Water Soluble ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Nucleic Acid Delivery

Short DNA represents an important class of biomacromolecules that are widely applied in gene therapy, editing, and modulation. However, the development of simple and reliable methods for their intracellular delivery remains a challenge. Herein, we describe that seven water-soluble, homogeneous supramolecular organic frameworks (SOFs) with a well-defined pore size and high stability in water that can accomplish in situ inclusion of single-stranded (ss) and double-stranded (ds) DNA (21, 23, and 58 nt) and effective intracellular delivery (including two noncancerous and six cancerous cell lines). Fluorescence quenching experiments for single and double end-labeled ss- and ds-DNA support that the DNA sequences can be completely enveloped by the SOFs. Confocal laser scanning microscopy and flow cytometry reveal that five of the SOFs exhibit excellent delivery efficiencies that, in most of the studied cases, outperform the commercial standard Lipo2000, even at low SOF–nucleic acid ratios. In addition to high delivery efficiencies, the water-soluble, self-assembled SOF carriers have a variety of advantages, including convenient preparation, high stability, and in situ DNA inclusion, which are all critical for practical applications in nucleic acid delivery.

Synthesis, Cellular Uptake, and Cytotoxicity of a Thermally Responsive Nanocapsule

2009 ◽  
Author(s):  
Wujie Zhang ◽  
Jianhua Rong ◽  
Qian Wang ◽  
Xiaoming He
Keyword(s):  
Cellular Uptake ◽  
Blood Cells ◽  
Mammalian Cells ◽  
Polymeric Nanoparticles ◽  
Intracellular Delivery ◽  
Sustained Delivery ◽  
Hydrogel Nanoparticles ◽  
Ambient Temperatures ◽  
Thermally Responsive ◽  
Therapeutic Drugs

Recently, polymeric nanoparticles have attracted tremendous interests as a useful tool to encapsulate therapeutic drugs, genes, and proteins for their controlled and sustained delivery. Among them, polymeric hydrogel nanoparticles with thermal and/or pH responsiveness have attracted particular attention [1]. Trehalose, a non-reducing disaccharide of glucose, has been demonstrated to be a potent, nontoxic bioprotectant for stabilizing lipids, proteins, viruses, and blood cells at cryogenic and particularly, ambient temperatures (i.e., cryo and lyopreservation) [2]. However, intracellular delivery of trehalose into small eukaryotic mammalian cells in a large quantity for biostabilization purpose has not been very successful so far [2]. In this study, a thermally responsive polymeric nanocapsule was synthesized and characterized with the aim to encapsulate trehalose for its intracellular delivery.

scholarly journals In Vivo Light-Driven DNA Binding and Cellular Uptake of Nucleic Acid Stains

2012 ◽  
Vol 7 (7) ◽  
pp. 1276-1280 ◽  
Author(s):  
Mateo I. Sánchez ◽  
José Martínez-Costas ◽  
Francisco Gonzalez ◽  
María A. Bermudez ◽  
M. Eugenio Vázquez ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Binding ◽  
Cellular Uptake

Magnetic silica spheres with large nanopores for nucleic acid adsorption and cellular uptake

Biomaterials ◽  
2012 ◽  
Vol 33 (3) ◽  
pp. 970-978 ◽  
Author(s):  
Jian Liu ◽  
Bo Wang ◽  
Sandy Budi Hartono ◽  
Tingting Liu ◽  
Phillip Kantharidis ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Cellular Uptake ◽  
Silica Spheres ◽  
Magnetic Silica

Head group configuration increases the biocompatibility of cationic lipids for nucleic acid delivery

2017 ◽  
Vol 5 (28) ◽  
pp. 5597-5607 ◽  
Author(s):  
Gerile Gerile ◽  
Tsogzolmaa Ganbold ◽  
Yizheng Li ◽  
Huricha Baigude
Keyword(s):  
Nucleic Acid ◽  
Therapeutic Approach ◽  
Genetic Diseases ◽  
Genetic Material ◽  
Intracellular Delivery ◽  
Cationic Lipids ◽  
Head Group ◽  
Nucleic Acid Delivery ◽  
Group Configuration

Intracellular delivery of genetic material is a potentially powerful therapeutic approach for the treatment of genetic diseases.

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