Oligoarginine vectors for intracellular delivery: Design and cellular-uptake mechanisms

Biopolymers ◽  
2006 ◽  
Vol 84 (3) ◽  
pp. 241-249 ◽  
Author(s):  
Shiroh Futaki
Keyword(s):  
Cellular Uptake ◽  
Intracellular Delivery ◽  
Uptake Mechanisms

Probing Cellular Outcomes Using Heterobivalent Constructs

2019 ◽  
Author(s):  
Rohit Bhadoria ◽  
Kefeng Ping ◽  
Christer Lohk ◽  
Ivar Järving ◽  
Pavel Starkov
Keyword(s):  
Cell Viability ◽  
Small Molecules ◽  
Cellular Uptake ◽  
Kinase Inhibitors ◽  
Rho Kinase ◽  
Intracellular Delivery ◽  
Rho Kinase Inhibitors

<div> <div> <div> <p>Conjugation techniques are central to improving intracellular delivery of bioactive small molecules. However, tracking and assessing the overall biological outcome of these constructs remains poorly understood. We addressed this issue by having developed a focused library of heterobivalent constructs based on Rho kinase inhibitors to probe various scenarios. By comparing induction of a phenotype of interest vs. cell viability vs. cellular uptake, we demonstrate that such conjugates indeed lead to divergent cellular outcomes. </p> </div> </div> </div>

scholarly journals In vitro dosimetry of agglomerates

Nanoscale ◽  
2014 ◽  
Vol 6 (13) ◽  
pp. 7325-7331 ◽  
Author(s):  
V. Hirsch ◽  
C. Kinnear ◽  
L. Rodriguez-Lorenzo ◽  
C. A. Monnier ◽  
B. Rothen-Rutishauser ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Diffusion Coefficients ◽  
Uptake Kinetics ◽  
Small Particles ◽  
Au Nps ◽  
Kinetics Of ◽  
Lower Diffusion ◽  
Uptake Mechanisms ◽  
In Vitro Dosimetry

A well-controlled route towards biocompatible agglomerated Au-NPs is reported, which span the range from small particles with high diffusion coefficients to larger particles with lower diffusion coefficients. Difference in uptake kinetics of single NPs and agglomerates can be explained by particokinetics, without the need to consider size-mediated cellular uptake mechanisms.

scholarly journals siRNA Inhibition of Endocytic Pathways to Characterize the Cellular Uptake Mechanisms of Folate-Functionalized Glycol Chitosan Nanogels

2015 ◽  
Vol 12 (6) ◽  
pp. 1970-1979 ◽  
Author(s):  
Paula Pereira ◽  
Sílvia S. Pedrosa ◽  
Jennifer M. Wymant ◽  
Edward Sayers ◽  
Alexandra Correia ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Glycol Chitosan ◽  
Uptake Mechanisms ◽  
Endocytic Pathways

Efficient cellular uptake of click nucleic acid modified proteins

2020 ◽  
Vol 56 (35) ◽  
pp. 4820-4823 ◽  
Author(s):  
Albert Harguindey ◽  
Heidi R. Culver ◽  
Jasmine Sinha ◽  
Christopher N. Bowman ◽  
Jennifer N. Cha
Keyword(s):  
Nucleic Acid ◽  
Cellular Uptake ◽  
Intracellular Delivery ◽  
Modified Proteins

Efficient intracellular delivery of biomacromolecules such as proteins continues to remain a challenge despite its potential for medicine.

Synthesis, Cellular Uptake, and Cytotoxicity of a Thermally Responsive Nanocapsule

2009 ◽  
Author(s):  
Wujie Zhang ◽  
Jianhua Rong ◽  
Qian Wang ◽  
Xiaoming He
Keyword(s):  
Cellular Uptake ◽  
Blood Cells ◽  
Mammalian Cells ◽  
Polymeric Nanoparticles ◽  
Intracellular Delivery ◽  
Sustained Delivery ◽  
Hydrogel Nanoparticles ◽  
Ambient Temperatures ◽  
Thermally Responsive ◽  
Therapeutic Drugs

Recently, polymeric nanoparticles have attracted tremendous interests as a useful tool to encapsulate therapeutic drugs, genes, and proteins for their controlled and sustained delivery. Among them, polymeric hydrogel nanoparticles with thermal and/or pH responsiveness have attracted particular attention [1]. Trehalose, a non-reducing disaccharide of glucose, has been demonstrated to be a potent, nontoxic bioprotectant for stabilizing lipids, proteins, viruses, and blood cells at cryogenic and particularly, ambient temperatures (i.e., cryo and lyopreservation) [2]. However, intracellular delivery of trehalose into small eukaryotic mammalian cells in a large quantity for biostabilization purpose has not been very successful so far [2]. In this study, a thermally responsive polymeric nanocapsule was synthesized and characterized with the aim to encapsulate trehalose for its intracellular delivery.

Cellular Uptake Mechanisms of Novel Anionic siRNA Lipoplexes

2012 ◽  
Vol 30 (4) ◽  
pp. 1161-1175 ◽  
Author(s):  
Mamta Kapoor ◽  
Diane J. Burgess
Keyword(s):  
Cellular Uptake ◽  
Uptake Mechanisms

scholarly journals Insights in cellular uptake mechanisms of pDNA–polycationic amphiphilic cyclodextrin nanoparticles (CDplexes)

2010 ◽  
Vol 143 (3) ◽  
pp. 318-325 ◽  
Author(s):  
Alejandro Díaz-Moscoso ◽  
Dries Vercauteren ◽  
Joanna Rejman ◽  
Juan M. Benito ◽  
Carmen Ortiz Mellet ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Uptake Mechanisms ◽  
Amphiphilic Cyclodextrin
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