A two-photon AIEgen for simultaneous dual-color imaging of atherosclerotic plaques

2019 ◽  
Vol 6 (3) ◽  
pp. 546-553 ◽  
Author(s):  
Bo Situ ◽  
Meng Gao ◽  
Xiaojing He ◽  
Shiwu Li ◽  
Bairong He ◽  
...  
Keyword(s):  
Atherosclerotic Plaques ◽  
Color Imaging ◽  
Two Photon ◽  
Dual Color

A smart color-switchable AIEgen for two-photon dual-color bioimaging of mouse atherosclerotic plaques with single-excitation is reported.

Two-photon dual-color imaging using fluorescent proteins

2008 ◽  
Vol 5 (5) ◽  
pp. 373-374 ◽  
Author(s):  
Hiroyuki Kawano ◽  
Takako Kogure ◽  
Yukiko Abe ◽  
Hideaki Mizuno ◽  
Atsushi Miyawaki
Keyword(s):  
Fluorescent Proteins ◽  
Color Imaging ◽  
Two Photon ◽  
Dual Color

Dual-Color Imaging of Sodium/Calcium Ion Activities with Two-Photon Fluorescent Probes

2010 ◽  
Vol 49 (38) ◽  
pp. 6786-6789 ◽  
Author(s):  
Hyung Joong Kim ◽  
Ji Hee Han ◽  
Mi Kyung Kim ◽  
Chang Su Lim ◽  
Hwan Myung Kim ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Calcium Ion ◽  
Color Imaging ◽  
Two Photon ◽  
Sodium Calcium ◽  
Dual Color ◽  
Ion Activities

Dual-color imaging of cytosolic and mitochondrial zinc ions in live tissues with two-photon fluorescent probes

2014 ◽  
Vol 12 (21) ◽  
pp. 3406-3412 ◽  
Author(s):  
Kailash Rathore ◽  
Chang Su Lim ◽  
Young Lee ◽  
Bong Rae Cho
Keyword(s):  
Fluorescent Probes ◽  
Live Cells ◽  
Zinc Ions ◽  
Color Imaging ◽  
Two Photon ◽  
Dual Color ◽  
Living Tissues

We have developed TP probes for [Zn2+]cyto and [Zn2+]mito, which emit TPEF at widely-separated wavelength regions. The new probes can simultaneously detect [Zn2+]cyto and [Zn2+]mito in live cells, as well as in living tissues by dual-color TPM imaging.

Dual-Color Imaging of Magnesium/Calcium Ion Activities with Two-Photon Fluorescent Probes

2012 ◽  
Vol 84 (19) ◽  
pp. 8110-8113 ◽  
Author(s):  
Xiaohu Dong ◽  
Ji Hee Han ◽  
Cheol Ho Heo ◽  
Hwan Myung Kim ◽  
Zhihong Liu ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Calcium Ion ◽  
Color Imaging ◽  
Two Photon ◽  
Dual Color ◽  
Ion Activities

Dual-Color Imaging of Sodium/Calcium Ion Activities with Two-Photon Fluorescent Probes

2010 ◽  
Vol 122 (38) ◽  
pp. 6938-6941 ◽  
Author(s):  
Hyung Joong Kim ◽  
Ji Hee Han ◽  
Mi Kyung Kim ◽  
Chang Su Lim ◽  
Hwan Myung Kim ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Calcium Ion ◽  
Color Imaging ◽  
Two Photon ◽  
Sodium Calcium ◽  
Dual Color ◽  
Ion Activities

scholarly journals Determining the optimal expression method for dual-color imaging

2021 ◽  
Vol 351 ◽  
pp. 109064
Author(s):  
Jacob F. Norman ◽  
Bahar Rahsepar ◽  
Jad Noueihed ◽  
John A. White
Keyword(s):  
Color Imaging ◽  
Dual Color

Integrating dual-color imaging capability into a monochromator

2004 ◽  
Vol 75 (1) ◽  
pp. 266-269 ◽  
Author(s):  
Henry Hess ◽  
Meher Antia ◽  
Viola Vogel
Keyword(s):  
Color Imaging ◽  
Imaging Capability ◽  
Dual Color

scholarly journals A Dual-Color Imaging System for Sugarcane Smut Fungus Sporisorium scitamineum

Plant Disease ◽  
2016 ◽  
Vol 100 (12) ◽  
pp. 2357-2362 ◽  
Author(s):  
Meixin Yan ◽  
Enping Cai ◽  
Jianuan Zhou ◽  
Changqing Chang ◽  
Pinggen Xi ◽  
...  
Keyword(s):  
Life Cycle ◽  
Fluorescent Protein ◽  
Imaging System ◽  
Abiotic Factors ◽  
Smut Fungus ◽  
Color Imaging ◽  
Sporisorium Scitamineum ◽  
Dual Color ◽  
Life Cycle Development ◽  
Whole Life Cycle

The life cycle of the sugarcane smut fungus Sporisorium scitamineum is a multistep process. Haploid sporidia of compatible (MAT-1 versus MAT-2) mating types fuse to generate pathogenic dikaryotic hyphae to infect the host. Within the host tissues, diploid teliospores are formed and induce a characteristic sorus that looks like a black whip. The diploid teliospores germinate to form haploid sporidia by meiosis. In order to monitor fungal development throughout the whole life cycle, we expressed the green fluorescent protein (GFP) and red fluorescent protein (RFP) in S. scitamineum MAT-1 and MAT-2 sporidia, respectively. Observation by epifluorescence microscope showed that conjugation tube formation and sporidia fusion occurred at 4 to 8 h, and formation of dikaryotic filaments was detected at 12 h after mating. The resultant teliospores, with diffused GFP and RFP, underwent meiosis as demonstrated by septated hypha with single fluorescent signal. We demonstrated that GFP- and RFP-tagged strains can be used to study the life cycle development of the fungal pathogen S. scitamineum, including the sexual mating and meiosis events. This dual-color imaging system would be a valuable tool for investigation of biotic and abiotic factors that might affect the fungal life cycle development and pathogenesis.

Single‐beam dual‐color alternate‐pathway two‐photon spectroscopy: Toward an optical toolbox for redox biology

2021 ◽  
Vol 52 (9) ◽  
pp. 1552-1560 ◽  
Author(s):  
Artem S. Chebotarev ◽  
Aleksandr A. Lanin ◽  
Roman I. Raevskii ◽  
Alexander I. Kostyuk ◽  
Daria D. Smolyarova ◽  
...  
Keyword(s):  
Single Beam ◽  
Redox Biology ◽  
Two Photon ◽  
Alternate Pathway ◽  
Dual Color

Two-photon excited fluorescence (TPEF) may be useful to identify macrophage subsets based on their metabolic activity and cellular responses in atherosclerotic plaques

2020 ◽  
Vol 309 ◽  
pp. 47-55
Author(s):  
Coraline Borowczyk ◽  
Jeanny Laroche-Traineau ◽  
Julien Brevier ◽  
Marie-Josée Jacobin-Valat ◽  
Sébastien Marais ◽  
...  
Keyword(s):  
Metabolic Activity ◽  
Cellular Responses ◽  
Atherosclerotic Plaques ◽  
Two Photon ◽  
Macrophage Subsets
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