Integrating dual-color imaging capability into a monochromator

2004 ◽  
Vol 75 (1) ◽  
pp. 266-269 ◽  
Author(s):  
Henry Hess ◽  
Meher Antia ◽  
Viola Vogel
Keyword(s):  
Color Imaging ◽  
Imaging Capability ◽  
Dual Color

scholarly journals Determining the optimal expression method for dual-color imaging

2021 ◽  
Vol 351 ◽  
pp. 109064
Author(s):  
Jacob F. Norman ◽  
Bahar Rahsepar ◽  
Jad Noueihed ◽  
John A. White
Keyword(s):  
Color Imaging ◽  
Dual Color

scholarly journals A Dual-Color Imaging System for Sugarcane Smut Fungus Sporisorium scitamineum

Plant Disease ◽  
2016 ◽  
Vol 100 (12) ◽  
pp. 2357-2362 ◽  
Author(s):  
Meixin Yan ◽  
Enping Cai ◽  
Jianuan Zhou ◽  
Changqing Chang ◽  
Pinggen Xi ◽  
...  
Keyword(s):  
Life Cycle ◽  
Fluorescent Protein ◽  
Imaging System ◽  
Abiotic Factors ◽  
Smut Fungus ◽  
Color Imaging ◽  
Sporisorium Scitamineum ◽  
Dual Color ◽  
Life Cycle Development ◽  
Whole Life Cycle

The life cycle of the sugarcane smut fungus Sporisorium scitamineum is a multistep process. Haploid sporidia of compatible (MAT-1 versus MAT-2) mating types fuse to generate pathogenic dikaryotic hyphae to infect the host. Within the host tissues, diploid teliospores are formed and induce a characteristic sorus that looks like a black whip. The diploid teliospores germinate to form haploid sporidia by meiosis. In order to monitor fungal development throughout the whole life cycle, we expressed the green fluorescent protein (GFP) and red fluorescent protein (RFP) in S. scitamineum MAT-1 and MAT-2 sporidia, respectively. Observation by epifluorescence microscope showed that conjugation tube formation and sporidia fusion occurred at 4 to 8 h, and formation of dikaryotic filaments was detected at 12 h after mating. The resultant teliospores, with diffused GFP and RFP, underwent meiosis as demonstrated by septated hypha with single fluorescent signal. We demonstrated that GFP- and RFP-tagged strains can be used to study the life cycle development of the fungal pathogen S. scitamineum, including the sexual mating and meiosis events. This dual-color imaging system would be a valuable tool for investigation of biotic and abiotic factors that might affect the fungal life cycle development and pathogenesis.

Binding-Directed Energy Transfer of Conjugated Polymer Materials for Dual-Color Imaging of Cell Membrane

2016 ◽  
Vol 28 (13) ◽  
pp. 4661-4669 ◽  
Author(s):  
Qianling Cui ◽  
Xiaoyu Wang ◽  
Yu Yang ◽  
Shengliang Li ◽  
Lidong Li ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Cell Membrane ◽  
Conjugated Polymer ◽  
Polymer Materials ◽  
Color Imaging ◽  
Dual Color ◽  
Directed Energy

scholarly journals Genetically-Encoded Yellow Fluorescent cAMP Indicator with an Expanded Dynamic Range for Dual-Color Imaging

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e100252 ◽  
Author(s):  
Haruki Odaka ◽  
Satoshi Arai ◽  
Takafumi Inoue ◽  
Tetsuya Kitaguchi
Keyword(s):  
Dynamic Range ◽  
Color Imaging ◽  
Dual Color

Photoswitchable fluorescent polymeric nanoparticles for rewritable fluorescence patterning and intracellular dual-color imaging with AIE-based fluorogens as FRET donors

2017 ◽  
Vol 8 (33) ◽  
pp. 4849-4855 ◽  
Author(s):  
Weibang Zhong ◽  
Xuyao Zeng ◽  
Jian Chen ◽  
Yongxiang Hong ◽  
Lehui Xiao ◽  
...  
Keyword(s):  
Polymeric Nanoparticles ◽  
Color Imaging ◽  
Dual Color

Photoswitchable fluorescent polymeric nanoparticles with AIE-based fluorogens as FRET donors were prepared for rewritable fluorescence patterning and intracellular dual-color imaging.

Two-photon dual-color imaging using fluorescent proteins

2008 ◽  
Vol 5 (5) ◽  
pp. 373-374 ◽  
Author(s):  
Hiroyuki Kawano ◽  
Takako Kogure ◽  
Yukiko Abe ◽  
Hideaki Mizuno ◽  
Atsushi Miyawaki
Keyword(s):  
Fluorescent Proteins ◽  
Color Imaging ◽  
Two Photon ◽  
Dual Color

Photochromic RAFT reagent helps construct superior photoswitchable fluorescent polymer nanoparticles for rewritable fluorescence patterning and intracellular dual-color imaging

2017 ◽  
Vol 8 (42) ◽  
pp. 6520-6526 ◽  
Author(s):  
Jian Chen ◽  
Weibang Zhong ◽  
Mingju Xue ◽  
Hong Wang ◽  
Maolin Yu ◽  
...  
Keyword(s):  
Chain Transfer ◽  
Polymeric Nanoparticles ◽  
Polymer Nanoparticles ◽  
Color Imaging ◽  
Dual Color ◽  
Fluorescent Polymer

Photoswitchable fluorescent polymeric nanoparticles with photochromic RAFT chain transfer reagent were synthesized for rewritable fluorescence patterning and intracellular dual color imaging.

Oligodots: Structurally Defined Fluorescent Nanoprobes for Multiscale Dual-Color Imaging in Vitro and in Vivo

2020 ◽  
Vol 12 (9) ◽  
pp. 10183-10192
Author(s):  
Fatemeh Ostadhossein ◽  
Dinabandhu Sar ◽  
Indu Tripathi ◽  
Julio Soares ◽  
Edward E. Remsen ◽  
...  
Keyword(s):  
Color Imaging ◽  
Dual Color

Dual-Color Imaging of Sodium/Calcium Ion Activities with Two-Photon Fluorescent Probes

2010 ◽  
Vol 49 (38) ◽  
pp. 6786-6789 ◽  
Author(s):  
Hyung Joong Kim ◽  
Ji Hee Han ◽  
Mi Kyung Kim ◽  
Chang Su Lim ◽  
Hwan Myung Kim ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Calcium Ion ◽  
Color Imaging ◽  
Two Photon ◽  
Sodium Calcium ◽  
Dual Color ◽  
Ion Activities

Dual-color imaging of nascent angiogenesis of pancreatic cancer in an orthotopic model

2006 ◽  
Vol 130 (2) ◽  
pp. 264-265
Author(s):  
Y. Amoh ◽  
L. Li ◽  
K. Tsuji ◽  
A.R. Moossa ◽  
K. Katsuoka ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Orthotopic Model ◽  
Color Imaging ◽  
Dual Color
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