Two-photon dual-color imaging using fluorescent proteins

2008 ◽  
Vol 5 (5) ◽  
pp. 373-374 ◽  
Author(s):  
Hiroyuki Kawano ◽  
Takako Kogure ◽  
Yukiko Abe ◽  
Hideaki Mizuno ◽  
Atsushi Miyawaki
Keyword(s):  
Fluorescent Proteins ◽  
Color Imaging ◽  
Two Photon ◽  
Dual Color

Dual-Color Imaging of Sodium/Calcium Ion Activities with Two-Photon Fluorescent Probes

2010 ◽  
Vol 49 (38) ◽  
pp. 6786-6789 ◽  
Author(s):  
Hyung Joong Kim ◽  
Ji Hee Han ◽  
Mi Kyung Kim ◽  
Chang Su Lim ◽  
Hwan Myung Kim ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Calcium Ion ◽  
Color Imaging ◽  
Two Photon ◽  
Sodium Calcium ◽  
Dual Color ◽  
Ion Activities

Dual-color imaging of cytosolic and mitochondrial zinc ions in live tissues with two-photon fluorescent probes

2014 ◽  
Vol 12 (21) ◽  
pp. 3406-3412 ◽  
Author(s):  
Kailash Rathore ◽  
Chang Su Lim ◽  
Young Lee ◽  
Bong Rae Cho
Keyword(s):  
Fluorescent Probes ◽  
Live Cells ◽  
Zinc Ions ◽  
Color Imaging ◽  
Two Photon ◽  
Dual Color ◽  
Living Tissues

We have developed TP probes for [Zn2+]cyto and [Zn2+]mito, which emit TPEF at widely-separated wavelength regions. The new probes can simultaneously detect [Zn2+]cyto and [Zn2+]mito in live cells, as well as in living tissues by dual-color TPM imaging.

Dual-Color Imaging of Magnesium/Calcium Ion Activities with Two-Photon Fluorescent Probes

2012 ◽  
Vol 84 (19) ◽  
pp. 8110-8113 ◽  
Author(s):  
Xiaohu Dong ◽  
Ji Hee Han ◽  
Cheol Ho Heo ◽  
Hwan Myung Kim ◽  
Zhihong Liu ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Calcium Ion ◽  
Color Imaging ◽  
Two Photon ◽  
Dual Color ◽  
Ion Activities

scholarly journals A new approach to dual-color two-photon microscopy with fluorescent proteins

2010 ◽  
Vol 10 (1) ◽  
pp. 6 ◽  
Author(s):  
Shane E Tillo ◽  
Thomas E Hughes ◽  
Nikolay S Makarov ◽  
Aleks Rebane ◽  
Mikhail Drobizhev
Keyword(s):  
Fluorescent Proteins ◽  
New Approach ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Dual Color

A two-photon AIEgen for simultaneous dual-color imaging of atherosclerotic plaques

2019 ◽  
Vol 6 (3) ◽  
pp. 546-553 ◽  
Author(s):  
Bo Situ ◽  
Meng Gao ◽  
Xiaojing He ◽  
Shiwu Li ◽  
Bairong He ◽  
...  
Keyword(s):  
Atherosclerotic Plaques ◽  
Color Imaging ◽  
Two Photon ◽  
Dual Color

A smart color-switchable AIEgen for two-photon dual-color bioimaging of mouse atherosclerotic plaques with single-excitation is reported.

scholarly journals LSSmScarlet, dCyRFP2s, dCyOFP2s and CRISPRed2s, Genetically Encoded Red Fluorescent Proteins with a Large Stokes Shift

2021 ◽  
Vol 22 (23) ◽  
pp. 12887
Author(s):  
Oksana M. Subach ◽  
Anna V. Vlaskina ◽  
Yuliya K. Agapova ◽  
Pavel V. Dorovatovskii ◽  
Alena Y. Nikolaeva ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Rational Design ◽  
Fluorescent Proteins ◽  
Stokes Shift ◽  
Confocal Imaging ◽  
Large Stokes Shift ◽  
Two Photon ◽  
Dual Color ◽  
Red Fluorescent Proteins

Genetically encoded red fluorescent proteins with a large Stokes shift (LSSRFPs) can be efficiently co-excited with common green FPs both under single- and two-photon microscopy, thus enabling dual-color imaging using a single laser. Recent progress in protein development resulted in a great variety of novel LSSRFPs; however, the selection of the right LSSRFP for a given application is hampered by the lack of a side-by-side comparison of the LSSRFPs’ performance. In this study, we employed rational design and random mutagenesis to convert conventional bright RFP mScarlet into LSSRFP, called LSSmScarlet, characterized by excitation/emission maxima at 470/598 nm. In addition, we utilized the previously reported LSSRFPs mCyRFP1, CyOFP1, and mCRISPRed as templates for directed molecular evolution to develop their optimized versions, called dCyRFP2s, dCyOFP2s and CRISPRed2s. We performed a quantitative assessment of the developed LSSRFPs and their precursors in vitro on purified proteins and compared their brightness at 488 nm excitation in the mammalian cells. The monomeric LSSmScarlet protein was successfully utilized for the confocal imaging of the structural proteins in live mammalian cells and multicolor confocal imaging in conjugation with other FPs. LSSmScarlet was successfully applied for dual-color two-photon imaging in live mammalian cells. We also solved the X-ray structure of the LSSmScarlet protein at the resolution of 1.4 Å that revealed a hydrogen bond network supporting excited-state proton transfer (ESPT). Quantum mechanics/molecular mechanics molecular dynamic simulations confirmed the ESPT mechanism of a large Stokes shift. Structure-guided mutagenesis revealed the role of R198 residue in ESPT that allowed us to generate a variant with improved pH stability. Finally, we showed that LSSmScarlet protein is not appropriate for STED microscopy as a consequence of LSSRed-to-Red photoconversion with high-power 775 nm depletion light.

Dual-Color Imaging of Sodium/Calcium Ion Activities with Two-Photon Fluorescent Probes

2010 ◽  
Vol 122 (38) ◽  
pp. 6938-6941 ◽  
Author(s):  
Hyung Joong Kim ◽  
Ji Hee Han ◽  
Mi Kyung Kim ◽  
Chang Su Lim ◽  
Hwan Myung Kim ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Calcium Ion ◽  
Color Imaging ◽  
Two Photon ◽  
Sodium Calcium ◽  
Dual Color ◽  
Ion Activities

scholarly journals Determining the optimal expression method for dual-color imaging

2021 ◽  
Vol 351 ◽  
pp. 109064
Author(s):  
Jacob F. Norman ◽  
Bahar Rahsepar ◽  
Jad Noueihed ◽  
John A. White
Keyword(s):  
Color Imaging ◽  
Dual Color

Integrating dual-color imaging capability into a monochromator

2004 ◽  
Vol 75 (1) ◽  
pp. 266-269 ◽  
Author(s):  
Henry Hess ◽  
Meher Antia ◽  
Viola Vogel
Keyword(s):  
Color Imaging ◽  
Imaging Capability ◽  
Dual Color
Sign in / Sign up

Export Citation Format

Share Document