Single‐beam dual‐color alternate‐pathway two‐photon spectroscopy: Toward an optical toolbox for redox biology

2021 ◽  
Vol 52 (9) ◽  
pp. 1552-1560 ◽  
Author(s):  
Artem S. Chebotarev ◽  
Aleksandr A. Lanin ◽  
Roman I. Raevskii ◽  
Alexander I. Kostyuk ◽  
Daria D. Smolyarova ◽  
...  
Keyword(s):  
Single Beam ◽  
Redox Biology ◽  
Two Photon ◽  
Alternate Pathway ◽  
Dual Color

Elimination of two-photon excited fluorescence using a single-beam coherent anti-Stokes Raman scattering setup

2013 ◽  
Vol 44 (10) ◽  
pp. 1379-1384 ◽  
Author(s):  
Alexander Wipfler ◽  
Jean Rehbinder ◽  
Tiago Buckup ◽  
Marcus Motzkus
Keyword(s):  
Raman Scattering ◽  
Single Beam ◽  
Two Photon ◽  
Anti Stokes

Single-beam femtosecond two-photon sequential writing and fluorescent readout in 2.5-D optical data storage

1998 ◽  
Author(s):  
N.I. Koroteev ◽  
S.A. Krikunov ◽  
S.A. Magnitskii ◽  
D.V. Malakhov ◽  
V.V. Shubin
Keyword(s):  
Data Storage ◽  
Optical Data Storage ◽  
Optical Data ◽  
Single Beam ◽  
Two Photon

Single-beam two-photon-recorded monolithic multilayer optical disks

2000 ◽  
Author(s):  
Haichuan Zhang ◽  
Alexander S. Dvornikov ◽  
Edwin P. Walker ◽  
Nam-Hyong Kim ◽  
Frederick B. McCormick, Jr.
Keyword(s):  
Single Beam ◽  
Two Photon ◽  
Optical Disks

Two-photon dual-color imaging using fluorescent proteins

2008 ◽  
Vol 5 (5) ◽  
pp. 373-374 ◽  
Author(s):  
Hiroyuki Kawano ◽  
Takako Kogure ◽  
Yukiko Abe ◽  
Hideaki Mizuno ◽  
Atsushi Miyawaki
Keyword(s):  
Fluorescent Proteins ◽  
Color Imaging ◽  
Two Photon ◽  
Dual Color

Two-Photon Single-Beam “Bit-by-Bit" Writing and Fluorescent “Page-by-Page" Reading 2.5D Femtosecond Optical Data Storage System

1998 ◽  
Vol 37 (Part 1, No. 4B) ◽  
pp. 2279-2280 ◽  
Author(s):  
Nikolay I. Koroteev ◽  
Sergei A. Krikunov ◽  
Sergei A. Magnitskii ◽  
Dmitriy V. Malakhov ◽  
Vladimir V. Shubin
Keyword(s):  
Data Storage ◽  
Storage System ◽  
Optical Data Storage ◽  
Optical Data ◽  
Single Beam ◽  
Two Photon ◽  
Data Storage System

scholarly journals Dual-color volumetric imaging of neural activity of cortical columns

2018 ◽  
Author(s):  
Shuting Han ◽  
Weijian Yang ◽  
Rafael Yuste
Keyword(s):  
Neural Activity ◽  
High Speed ◽  
Neural Circuits ◽  
Emergent Properties ◽  
Spatiotemporal Resolution ◽  
Population Activity ◽  
Volumetric Imaging ◽  
Two Photon ◽  
Dual Color

To capture the emergent properties of neural circuits, high-speed volumetric imaging of neural activity at cellular resolution is desirable. But while conventional two-photon calcium imaging is a powerful tool to study population activity in vivo, it is restrained to two-dimensional planes. Expanding it to 3D while maintaining high spatiotemporal resolution appears necessary. Here, we developed a two-photon microscope with dual-color laser excitation that can image neural activity in a 3D volume. We imaged the neuronal activity of primary visual cortex from awake mice, spanning from L2 to L5 with 10 planes, at a rate of 10 vol/sec, and demonstrated volumetric imaging of L1 long-range PFC projections and L2/3 somatas. Using this method, we map visually-evoked neuronal ensembles in 3D, finding a lack of columnar structure in orientation responses and revealing functional correlations between cortical layers which differ from trial to trial and are missed in sequential imaging. We also reveal functional interactions between presynaptic L1 axons and postsynaptic L2/3 neurons. Volumetric two-photon imaging appears an ideal method for functional connectomics of neural circuits.

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