Detecting miRNA biomarkers from extracellular vesicles for cardiovascular disease with a microfluidic system

Lab on a Chip ◽  
2018 ◽  
Vol 18 (19) ◽  
pp. 2917-2925 ◽  
Author(s):  
Hong-Lin Cheng ◽  
Chien-Yu Fu ◽  
Wen-Che Kuo ◽  
Yen-Wen Chen ◽  
Yi-Sin Chen ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Cardiovascular Diseases ◽  
Early Detection ◽  
Extracellular Vesicles ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Extracellular Vesicle ◽  
Microfluidic System ◽  
Microfluidic Platform ◽  
System P

A novel microfluidic platform for extracellular vesicle extraction, microRNA isolation and detection with field-effect transistors for early detection of cardiovascular diseases.

A microfluidic platform integrated with field-effect transistors for enumeration of circulating tumor cells

Lab on a Chip ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 618-625 ◽  
Author(s):  
Yi-Hong Chen ◽  
Anil Kumar Pulikkathodi ◽  
Yu-Dong Ma ◽  
Yu-Lin Wang ◽  
Gwo-Bin Lee
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Automatic Detection ◽  
Microfluidic System ◽  
Microfluidic Platform

A new integrated microfluidic system was developed for automatic detection and enumeration of CTCs with field-effect transistors (FETs).

An integrated microfluidic system for on-chip enrichment and quantification of circulating extracellular vesicles from whole blood

Lab on a Chip ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 3305-3315 ◽  
Author(s):  
Yi-Sin Chen ◽  
Yu-Dong Ma ◽  
Chihchen Chen ◽  
Shu-Chu Shiesh ◽  
Gwo-Bin Lee
Keyword(s):  
Extracellular Vesicles ◽  
Immunosorbent Assay ◽  
Whole Blood ◽  
Magnetic Beads ◽  
Extracellular Vesicle ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Whole Blood ◽  
On Chip ◽  
Chip Enrichment

An integrated microfluidic system was developed for extracellular vesicle (EV) enrichment and quantification by using anti-CD63-coated magnetic beads and an on-chip enzyme-linked immunosorbent assay in human whole blood.

scholarly journals Differential clusterization of soluble and extracellular vesicle-associated cytokines in myocardial infarction

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Anna Lebedeva ◽  
Wendy Fitzgerald ◽  
Ivan Molodtsov ◽  
Alexander Shpektor ◽  
Elena Vasilieva ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Cardiovascular Diseases ◽  
Extracellular Vesicles ◽  
Inflammatory Cytokines ◽  
Extracellular Vesicle ◽  
Plasma Samples ◽  
Cytokine Release ◽  
St Elevation ◽  
Infectious Etiology ◽  
Pro Inflammatory Cytokines

AbstractA proinflammatory dysregulation of cytokine release is associated with various diseases, in particular with those of infectious etiology, as well as with cardiovascular diseases (CVD). We showed earlier that cytokines are released in two forms, soluble and in association with extracellular vesicles (EVs). Here, we investigated the patterns of expression and clustering of soluble and EV-associated cytokines in patients with ST-elevation myocardial infarction (STEMI). We collected plasma samples from 48 volunteers without CVD and 62 patients with STEMI, separated soluble and EV fractions, and analyzed them for 33 cytokines using a multiplexed bead-based assay. We identified soluble and EV-associated cytokines that are upregulated in STEMI and form correlative clusters. Several clustered soluble cytokines were expressed almost exclusively in patients with STEMI. EV-associated cytokines were largely not affected by STEMI, except for pro-inflammatory cytokines IL-6, IL-18, and MIG, as well as anti-inflammatory IL-2 that were upregulated in a correlated fashion. Our results demonstrated that soluble cytokines in patients with STEMI are upregulated in a coordinated fashion in contrast to the mainly unaffected system of EV-associated cytokines. Identification of cytokine clusters affected differently by STEMI now permits investigation of their differential contributions to this pathology.

scholarly journals Graphene-Based Biosensor for Early Detection of Iron Deficiency

Sensors ◽  
2020 ◽  
Vol 20 (13) ◽  
pp. 3688 ◽  
Author(s):  
Oluwadamilola Oshin ◽  
Dmitry Kireev ◽  
Hanna Hlukhova ◽  
Francis Idachaba ◽  
Deji Akinwande ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Early Detection ◽  
Field Effect ◽  
Production Cost ◽  
Field Effect Transistors ◽  
Deficiency Anemia ◽  
Nutritional Disorder ◽  
Non Invasive ◽  
Fast Prototyping ◽  
Succinimidyl Ester

Iron deficiency (ID) is the most prevalent and severe nutritional disorder globally and is the leading cause of iron deficiency anemia (IDA). IDA often progresses subtly symptomatic in children, whereas prolonged deficiency may permanently impair development. Early detection and frequent screening are, therefore, essential to avoid the consequences of IDA. In order to reduce the production cost and complexities involved in building advanced ID sensors, the devices were fabricated using a home-built patterning procedure that was developed and used for this work instead of lithography, which allows for fast prototyping of dimensions. In this article, we report the development of graphene-based field-effect transistors (GFETs) functionalized with anti-ferritin antibodies through a linker molecule (1-pyrenebutanoic acid, succinimidyl ester), to facilitate specific conjugation with ferritin antigen. The resulting biosensors feature an unprecedented ferritin detection limit of 10 fM, indicating a tremendous potential for non-invasive (e.g., saliva) ferritin detection.

Simultaneous detection of multiple NT-proBNP clinical samples utilizing an aptamer-based sandwich assay on an integrated microfluidic system

Lab on a Chip ◽  
2019 ◽  
Vol 19 (9) ◽  
pp. 1676-1685 ◽  
Author(s):  
Anirban Sinha ◽  
Priya Gopinathan ◽  
Yi-Da Chung ◽  
Shu-Chu Shiesh ◽  
Gwo-Bin Lee
Keyword(s):  
Heart Failure ◽  
Simultaneous Detection ◽  
Microfluidic System ◽  
Failure Diagnosis ◽  
Clinical Samples ◽  
Sandwich Assay ◽  
Microfluidic Platform ◽  
System P ◽  
Heart Failure Diagnosis

An integrated microfluidic platform was developed for the simultaneous detection of NT-proBNP, a widely studied biomarker for heart failure diagnosis, in up to six clinical samples within 25 min by using a novel aptamer-based sandwich assay.

scholarly journals Detection of C-reactive protein on an integrated microfluidic system by utilizing field-effect transistors and aptamers

2017 ◽  
Vol 11 (4) ◽  
pp. 044105 ◽  
Author(s):  
Wei-Chieh Kao ◽  
Yen-Wen Chen ◽  
Chia-Ho Chu ◽  
Wen-Hsin Chang ◽  
Shu-Chu Shiesh ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistors ◽  
Microfluidic System ◽  
C Reactive Protein ◽  
Reactive Protein
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