A microfluidic platform integrated with field-effect transistors for enumeration of circulating tumor cells

Lab on a Chip ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 618-625 ◽  
Author(s):  
Yi-Hong Chen ◽  
Anil Kumar Pulikkathodi ◽  
Yu-Dong Ma ◽  
Yu-Lin Wang ◽  
Gwo-Bin Lee
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Automatic Detection ◽  
Microfluidic System ◽  
Microfluidic Platform

A new integrated microfluidic system was developed for automatic detection and enumeration of CTCs with field-effect transistors (FETs).

Detecting miRNA biomarkers from extracellular vesicles for cardiovascular disease with a microfluidic system

Lab on a Chip ◽  
2018 ◽  
Vol 18 (19) ◽  
pp. 2917-2925 ◽  
Author(s):  
Hong-Lin Cheng ◽  
Chien-Yu Fu ◽  
Wen-Che Kuo ◽  
Yen-Wen Chen ◽  
Yi-Sin Chen ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Cardiovascular Diseases ◽  
Early Detection ◽  
Extracellular Vesicles ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Extracellular Vesicle ◽  
Microfluidic System ◽  
Microfluidic Platform ◽  
System P

A novel microfluidic platform for extracellular vesicle extraction, microRNA isolation and detection with field-effect transistors for early detection of cardiovascular diseases.

scholarly journals Automatic detection of circulating tumor cells in darkfield microscopic images of unstained blood using boosting techniques

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0208385 ◽  
Author(s):  
Anca Ciurte ◽  
Cristina Selicean ◽  
Olga Soritau ◽  
Rares Buiga
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Automatic Detection ◽  
Microscopic Images

Spectrally Combined Encoding for Profiling Heterogeneous Circulating Tumor Cells Using a Multifunctional Nanosphere‐Mediated Microfluidic Platform

2020 ◽  
Vol 59 (28) ◽  
pp. 11240-11244
Author(s):  
Ling‐Ling Wu ◽  
Zhi‐Ling Zhang ◽  
Man Tang ◽  
Dong‐Liang Zhu ◽  
Xiao‐Juan Dong ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Platform

scholarly journals Functional profiling of circulating tumor cells with an integrated vortex capture and single-cell protease activity assay

2018 ◽  
Vol 115 (40) ◽  
pp. 9986-9991 ◽  
Author(s):  
Manjima Dhar ◽  
Jeffrey Nam Lam ◽  
Tonya Walser ◽  
Steven M. Dubinett ◽  
Matthew B. Rettig ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Activity Ratio ◽  
Proteolytic Enzymes ◽  
Microfluidic System ◽  
Matrix Metalloprotease ◽  
Rare Cancer ◽  
Companion Diagnostics ◽  
Functional Profiling ◽  
Vortex Capture

Tumor cells are hypothesized to use proteolytic enzymes to facilitate invasion. Whether circulating tumor cells (CTCs) secrete these enzymes to aid metastasis is unknown. A quantitative and high-throughput approach to assay CTC secretion is needed to address this question. We developed an integrated microfluidic system that concentrates rare cancer cells >100,000-fold from 1 mL of whole blood into ∼50,000 2-nL drops composed of assay reagents within 15 min. The system isolates CTCs by size, exchanges fluid around CTCs to remove contaminants, introduces a matrix metalloprotease (MMP) substrate, and encapsulates CTCs into microdroplets. We found CTCs from prostate cancer patients possessed above baseline levels of MMP activity (1.7- to 200-fold). Activity of CTCs was generally higher than leukocytes from the same patient (average CTC/leukocyte MMP activity ratio, 2.6 ± 1.5). Higher MMP activity of CTCs suggests active proteolytic processes that may facilitate invasion or immune evasion and be relevant phenotypic biomarkers enabling companion diagnostics for anti-MMP therapies.

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