Rational design of a lipid-droplet-polarity based fluorescent probe for potential cancer diagnosis

2018 ◽  
Vol 54 (85) ◽  
pp. 12093-12096 ◽  
Author(s):  
Junling Yin ◽  
Min Peng ◽  
Yanyan Ma ◽  
Rui Guo ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probe ◽  
Lipid Droplet ◽  
Cancer Diagnosis ◽  
Rational Design ◽  
High Specificity ◽  
Diagnosis Of Cancer ◽  
First Time ◽  
Potential Cancer

We have rationally designed a robust fluorescent probe CTPA for potential cancer diagnosis by monitoring LD numbers and polarity variation. With the outstanding solvatochromism and high specificity for LDs of CTPA, the diagnosis of cancer can be achieved not only at the cellular levels but also in organs and living mice for the first time.

Rational design of a fast and selective near-infrared fluorescent probe for targeted monitoring of endogenous nitric oxide

2017 ◽  
Vol 53 (76) ◽  
pp. 10520-10523 ◽  
Author(s):  
Junma Tang ◽  
Zhiqian Guo ◽  
Yutao Zhang ◽  
Bing Bai ◽  
Wei-Hong Zhu
Keyword(s):  
Nitric Oxide ◽  
Fluorescent Probe ◽  
Rational Design ◽  
Near Infrared ◽  
High Specificity ◽  
Fast Response ◽  
Turn On ◽  
Endogenous Nitric Oxide

We developed a fast and selective near-infrared (NIR) fluorescent probe for the targeted tracing of endogenous NO which possesses vital features including a significant turn-on NIR response, high specificity, and a fast response.

Tracking lipid droplet dynamics for the discrimination of cancer cells by a solvatochromic fluorescent probe

2021 ◽  
Vol 333 ◽  
pp. 129541
Author(s):  
Linlin Yang ◽  
Jianping Wang ◽  
Bianhua Liu ◽  
Guangmei Han ◽  
Hong Wang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probe ◽  
Lipid Droplet ◽  
Droplet Dynamics

Engineering dithiobenzoic acid lactone-decorated Si-rhodamine as a highly selective near-infrared HOCl fluorescent probe for imaging drug-induced acute nephrotoxicity

2019 ◽  
Vol 55 (73) ◽  
pp. 10916-10919 ◽  
Author(s):  
Jinping Wang ◽  
Dan Cheng ◽  
Longmin Zhu ◽  
Peng Wang ◽  
Hong-Wen Liu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Drug Induced ◽  
Acid Lactone ◽  
First Time

A NIR fluorescent probe based on Si-rhodamine dithiobenzoic acid lactone was used to selectively visualize HOCl during GEN-induced nephrotoxicity for the first time.

scholarly journals Hydrogen Sulfide Increases Nitric Oxide Production and Subsequent S-Nitrosylation in Endothelial Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ping-Ho Chen ◽  
Yaw-Syan Fu ◽  
Yun-Ming Wang ◽  
Kun-Han Yang ◽  
Danny Ling Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Hydrogen Sulfide ◽  
Protein Kinase ◽  
Fluorescent Probe ◽  
Acid Methyl Ester ◽  
High Specificity ◽  
Protein S ◽  
No Production ◽  
No Donor

Hydrogen sulfide (H2S) and nitric oxide (NO), two endogenous gaseous molecules in endothelial cells, got increased attention with respect to their protective roles in the cardiovascular system. However, the details of the signaling pathways between H2S and NO in endothelia cells remain unclear. In this study, a treatment with NaHS profoundly increased the expression and the activity of endothelial nitric oxide synthase. Elevated gaseous NO levels were observed by a novel and specific fluorescent probe, 5-amino-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid methyl ester (FA-OMe), and quantified by flow cytometry. Further study indicated an increase of upstream regulator for eNOS activation, AMP-activated protein kinase (AMPK), and protein kinase B (Akt). By using a biotin switch, the level of NO-mediated protein S-nitrosylation was also enhanced. However, with the addition of the NO donor, NOC-18, the expressions of cystathionine-γ-lyase, cystathionine-β-synthase, and 3-mercaptopyruvate sulfurtransferase were not changed. The level of H2S was also monitored by a new designed fluorescent probe, 4-nitro-7-thiocyanatobenz-2-oxa-1,3-diazole (NBD-SCN) with high specificity. Therefore, NO did not reciprocally increase the expression of H2S-generating enzymes and the H2S level. The present study provides an integrated insight of cellular responses to H2S and NO from protein expression to gaseous molecule generation, which indicates the upstream role of H2S in modulating NO production and protein S-nitrosylation.

A fluorescent probe for relay recognition of homocysteine and Group IIIA ions including Ga(iii)

2016 ◽  
Vol 52 (4) ◽  
pp. 827-830 ◽  
Author(s):  
Ya-Wen Wang ◽  
Shun-Bang Liu ◽  
Wei-Jian Ling ◽  
Yu Peng
Keyword(s):  
Fluorescent Probe ◽  
First Time

Relay fluorescence recognition of homocysteine and gallium ions was realized by sequential chemodosimeter and chemosensor approaches for the first time.

scholarly journals Digital Interventions to Support Adolescents and Young Adults With Cancer: Systematic Review

JMIR Cancer ◽  
10.2196/12071 ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. e12071 ◽  
Author(s):  
Lisa McCann ◽  
Kathryn Anne McMillan ◽  
Gemma Pugh
Keyword(s):  
Young Adults ◽  
Cancer Diagnosis ◽  
Subjective Experience ◽  
Emotional Health ◽  
Digital Health ◽  
Health Interventions ◽  
Adolescents And Young Adults ◽  
Research Papers ◽  
Digital Interventions ◽  
Diagnosis Of Cancer

Background The last decade has seen an increase in the number of digital health interventions designed to support adolescents and young adults (AYAs) with cancer. Objective The objective of this review was to identify, characterize, and fully assess the quality, feasibility, and efficacy of existing digital health interventions developed specifically for AYAs, aged between 13 and 39 years, living with or beyond a cancer diagnosis. Methods Searches were performed in PubMed, EMBASE, and Web of Science to identify digital health interventions designed specifically for AYA living with or beyond a cancer diagnosis. Data on the characteristics and outcomes of each intervention were synthesized. Results A total of 4731 intervention studies were identified through the searches; 38 interventions (43 research papers) met the inclusion criteria. Most (20/38, 53%) were website-based interventions. Most studies focused on symptom management and medication adherence (15, 39%), behavior change (15, 39%), self-care (8, 21%), and emotional health (7, 18%). Most digital health interventions included multiple automated and communicative functions such as enriched information environments, automated follow-up messages, and access to peer support. Where reported (20, 53% of studies), AYAs’ subjective experience of using the digital platform was typically positive. The overall quality of the studies was found to be good (mean Quality Assessment Criteria for Evaluating Primary Research Papers from a Variety of Fields scores >68%). Some studies reported feasibility outcomes (uptake, acceptability, and attrition) but were not sufficiently powered to comment on intervention effects. Conclusions Numerous digital interventions have been developed and designed to support young people living with and beyond a diagnosis of cancer. However, many of these interventions have yet to be deployed, implemented, and evaluated at scale.

scholarly journals Should the Endoscopic Restrictions during COVID-19 Pandemic Remain Unchanged?

2021 ◽  
Vol 12 (3) ◽  
pp. 358-365
Author(s):  
Emilio Bresky ◽  
Gustavo Bresky ◽  
Domingo Lancellotti ◽  
Juan Madariaga ◽  
Sebastian Licuime ◽  
...  
Keyword(s):  
Cancer Diagnosis ◽  
Statistical Tests ◽  
Endoscopic Diagnosis ◽  
A Posteriori ◽  
Risk Of Infection ◽  
Gastrointestinal Cancers ◽  
Digestive Cancer ◽  
Endoscopic Procedures ◽  
Potential Cancer ◽  
Strict Protocol

(Background) Endoscopic procedures are interventions that have been defined as carrying a high-risk of infection with COVID-19. Most endoscopy units restrict their activity based on pre-endoscopic diagnosis. (Objective) To determine the consequences of endoscopic restrictions as a result of the COVID-19 pandemic and their impact on digestive cancer diagnosis. (Design) A comparison of upper digestive endoscopies and colonoscopies with gastrointestinal cancers diagnosed between three endoscopic centers, two of which restricted their procedures and one that did not but performed the procedures under a strict protocol. (Setting) A retrospective analysis was performed collecting data between 15 March 2019 and 15 August 2020. Two-factor ANOVA and a Tukey’s a posteriori test were used as statistical tests. (Main outcome measures) There was variation in gastrointestinal cancer diagnosis between 2019 and 2020, considering the endoscopic procedures performed each year. (Result) There was a significant decrease in the total endoscopic procedures performed between 2019 and 2020 (p < 0.001), the result of reduced testing at the two centers (p < 0.001) with pre-endoscopic restrictions, which was not compensated for by a slight increase in procedures at the center without restrictions (p = 0.139). Regarding the total cancers diagnosed, while a significant decrease was observed for the two centers with pre-endoscopic restrictions (p = 0.007), a significant increase was registered in the center that maintained its endoscopic productivity (p < 0.001). After 851 procedures (537 upper digestive endoscopies and 314 colonoscopies) there was no evidence of COVID-19 infection in the endoscopic staff. (Conclusion) Endoscopic restrictions based on pre-endoscopic diagnosis should be reassessed in consideration of local pandemic situations, and a balance should be sought between COVID-19 infection risk and the detrimental delay of potential cancer diagnosis.

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