Rational design of a fast and selective near-infrared fluorescent probe for targeted monitoring of endogenous nitric oxide

2017 ◽  
Vol 53 (76) ◽  
pp. 10520-10523 ◽  
Author(s):  
Junma Tang ◽  
Zhiqian Guo ◽  
Yutao Zhang ◽  
Bing Bai ◽  
Wei-Hong Zhu
Keyword(s):  
Nitric Oxide ◽  
Fluorescent Probe ◽  
Rational Design ◽  
Near Infrared ◽  
High Specificity ◽  
Fast Response ◽  
Turn On ◽  
Endogenous Nitric Oxide

We developed a fast and selective near-infrared (NIR) fluorescent probe for the targeted tracing of endogenous NO which possesses vital features including a significant turn-on NIR response, high specificity, and a fast response.

scholarly journals Selective and sensitive visualization of endogenous nitric oxide in living cells and animals by a Si-rhodamine deoxylactam-based near-infrared fluorescent probe

2017 ◽  
Vol 8 (10) ◽  
pp. 6857-6864 ◽  
Author(s):  
Yingying Huo ◽  
Junfeng Miao ◽  
Lingjun Han ◽  
Yaping Li ◽  
Zhe Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fluorescent Probe ◽  
Mouse Models ◽  
Near Infrared ◽  
Living Cells ◽  
Endogenous Nitric Oxide

A Si-rhodamine deoxylactam-based near-infrared fluorescent probe has been successfully developed for the imaging of endogenous NO in living cells and mouse models.

scholarly journals Hydrogen Sulfide Increases Nitric Oxide Production and Subsequent S-Nitrosylation in Endothelial Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ping-Ho Chen ◽  
Yaw-Syan Fu ◽  
Yun-Ming Wang ◽  
Kun-Han Yang ◽  
Danny Ling Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Hydrogen Sulfide ◽  
Protein Kinase ◽  
Fluorescent Probe ◽  
Acid Methyl Ester ◽  
High Specificity ◽  
Protein S ◽  
No Production ◽  
No Donor

Hydrogen sulfide (H2S) and nitric oxide (NO), two endogenous gaseous molecules in endothelial cells, got increased attention with respect to their protective roles in the cardiovascular system. However, the details of the signaling pathways between H2S and NO in endothelia cells remain unclear. In this study, a treatment with NaHS profoundly increased the expression and the activity of endothelial nitric oxide synthase. Elevated gaseous NO levels were observed by a novel and specific fluorescent probe, 5-amino-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid methyl ester (FA-OMe), and quantified by flow cytometry. Further study indicated an increase of upstream regulator for eNOS activation, AMP-activated protein kinase (AMPK), and protein kinase B (Akt). By using a biotin switch, the level of NO-mediated protein S-nitrosylation was also enhanced. However, with the addition of the NO donor, NOC-18, the expressions of cystathionine-γ-lyase, cystathionine-β-synthase, and 3-mercaptopyruvate sulfurtransferase were not changed. The level of H2S was also monitored by a new designed fluorescent probe, 4-nitro-7-thiocyanatobenz-2-oxa-1,3-diazole (NBD-SCN) with high specificity. Therefore, NO did not reciprocally increase the expression of H2S-generating enzymes and the H2S level. The present study provides an integrated insight of cellular responses to H2S and NO from protein expression to gaseous molecule generation, which indicates the upstream role of H2S in modulating NO production and protein S-nitrosylation.

A novel near-infrared “turn-on” fluorescent probe for selective detection of Fe3+ and its application in vitro imaging

2019 ◽  
Vol 207 ◽  
pp. 613-619 ◽  
Author(s):  
Xiaofei Gu ◽  
Xiuxuan Zhang ◽  
Zhe Liu ◽  
Yu Cheng ◽  
Fulong Ma ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Selective Detection ◽  
Turn On

Near-infrared templated fluorescent probe for nitroxyl: Selective and sensitive turn-on detection in living cells

2020 ◽  
Vol 310 ◽  
pp. 127839 ◽  
Author(s):  
Sathyadevi Palanisamy ◽  
Li-Fong Chen ◽  
Shey-Cherng Tzou ◽  
Yun-Ming Wang
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Living Cells ◽  
Turn On

A turn-on near-infrared fluorescent probe for detection of cysteine over glutathione and homocysteine in vivo

2019 ◽  
Vol 11 (14) ◽  
pp. 1857-1867 ◽  
Author(s):  
Qing He ◽  
Ruixi Li ◽  
Zhenwei Yuan ◽  
Habtamu Kassaye ◽  
Jinrong Zheng ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Turn On

Cysteine (Cys) has emerged as a significant sensing target in recent years.

A fast-response two-photon fluorescent probe for the detection of Cys over GSH/Hcy with a large turn-on signal and its application in living tissues

2017 ◽  
Vol 5 (1) ◽  
pp. 134-138 ◽  
Author(s):  
Jian-Yong Wang ◽  
Zhan-Rong Liu ◽  
Mingguang Ren ◽  
Xiuqi Kong ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probe ◽  
Fast Response ◽  
Living Cells ◽  
Two Photon ◽  
Turn On ◽  
Living Tissues

We developed a novel two-photon fluorescent probe for sensing Cys specifically. The two-photon fluorescent probe exhibited favorable properties and was successfully applied for imaging Cys in living cells and tissues.

A highly selective turn-on near-infrared fluorescent probe for hydrogen sulfide detection and imaging in living cells

2012 ◽  
Vol 48 (96) ◽  
pp. 11757 ◽  
Author(s):  
Rui Wang ◽  
Fabiao Yu ◽  
Lingxin Chen ◽  
Hao Chen ◽  
Linjie Wang ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Living Cells ◽  
Turn On
Sign in / Sign up

Export Citation Format

Share Document