scholarly journals Hydrogel-droplet microfluidic platform for high-resolution imaging and sorting of early larval Caenorhabditis elegans

Lab on a Chip ◽  
2015 ◽  
Vol 15 (6) ◽  
pp. 1424-1431 ◽  
Author(s):  
Guillaume Aubry ◽  
Mei Zhan ◽  
Hang Lu
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
Microfluidic Device ◽  
High Resolution Imaging ◽  
Microfluidic Platform ◽  
C Elegans ◽  
Resolution Imaging

We present a microfluidic device for high-resolution imaging and sorting of early larval C. elegans. The animals are isolated in droplets and temporarily immobilized for imaging using a reversible hydrogel.

scholarly journals Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap

Lab on a Chip ◽  
2017 ◽  
Vol 17 (6) ◽  
pp. 1116-1127 ◽  
Author(s):  
Adam L. Nekimken ◽  
Holger Fehlauer ◽  
Anna A. Kim ◽  
Sandra N. Manosalvas-Kjono ◽  
Purim Ladpli ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
Neuronal Activity ◽  
High Resolution Imaging ◽  
C Elegans ◽  
Resolution Imaging ◽  
Simultaneous Immobilization ◽  
Stimulation Of

A new microfluidic tool for simultaneous immobilization, force delivery and high resolution imaging of neuronal activity in living Caenorhabditis elegans.

On-demand dielectrophoretic immobilization and high-resolution imaging of C. elegans in microfluids

2018 ◽  
Vol 259 ◽  
pp. 703-708 ◽  
Author(s):  
Liang Huang ◽  
Peng Zhao ◽  
Junhan Wu ◽  
Han-Sheng Chuang ◽  
Wenhui Wang
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
C Elegans ◽  
On Demand ◽  
Resolution Imaging

scholarly journals Live-cell vibrational imaging of choline metabolites by stimulated Raman scattering coupled with isotope-based metabolic labeling

The Analyst ◽  
2014 ◽  
Vol 139 (10) ◽  
pp. 2312-2317 ◽  
Author(s):  
Fanghao Hu ◽  
Lu Wei ◽  
Chaogu Zheng ◽  
Yihui Shen ◽  
Wei Min
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Mammalian Cells ◽  
Stimulated Raman Scattering ◽  
Primary Neurons ◽  
High Resolution Imaging ◽  
Metabolic Labeling ◽  
C Elegans ◽  
Resolution Imaging

High-resolution imaging of choline metabolites in living mammalian cells, primary neurons andC. eleganshas been demonstrated with the potential forin vivodisease detection and developmental monitoring.

scholarly journals Assembly of Caenorhabditis elegans acentrosomal spindles occurs without evident microtubule-organizing centers and requires microtubule sorting by KLP-18/kinesin-12 and MESP-1

2016 ◽  
Vol 27 (20) ◽  
pp. 3122-3131 ◽  
Author(s):  
Ian D. Wolff ◽  
Michael V. Tran ◽  
Timothy J. Mullen ◽  
Anne M. Villeneuve ◽  
Sarah M. Wignall
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
Nuclear Envelope ◽  
Spindle Assembly ◽  
Cell Types ◽  
High Resolution Imaging ◽  
Spindle Formation ◽  
Mixed Polarity ◽  
Resolution Imaging ◽  
Meiosis I

Although centrosomes contribute to spindle formation in most cell types, oocytes of many species are acentrosomal and must organize spindles in their absence. Here we investigate this process in Caenorhabditis elegans, detailing how acentrosomal spindles form and revealing mechanisms required to establish bipolarity. Using high-resolution imaging, we find that in meiosis I, microtubules initially form a “cage-like” structure inside the disassembling nuclear envelope. This structure reorganizes so that minus ends are sorted to the periphery of the array, forming multiple nascent poles that then coalesce until bipolarity is achieved. In meiosis II, microtubules nucleate in the vicinity of chromosomes but then undergo similar sorting and pole formation events. We further show that KLP-18/kinesin-12 and MESP-1, previously shown to be required for spindle bipolarity, likely contribute to bipolarity by sorting microtubules. After their depletion, minus ends are not sorted outward at the early stages of spindle assembly and instead converge. These proteins colocalize on microtubules, are interdependent for localization, and can interact, suggesting that they work together. We propose that KLP-18/kinesin-12 and MESP-1 form a complex that functions to sort microtubules of mixed polarity into a configuration in which minus ends are away from the chromosomes, enabling formation of nascent poles.

scholarly journals Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

10.3791/56530 ◽  
2018 ◽  
Author(s):  
Holger Fehlauer ◽  
Adam L. Nekimken ◽  
Anna A. Kim ◽  
Beth L. Pruitt ◽  
Miriam B. Goodman ◽  
...  
Keyword(s):  
High Resolution ◽  
Mechanical Stimulation ◽  
High Resolution Imaging ◽  
C Elegans ◽  
Resolution Imaging

scholarly journals On-demand optical immobilization of Caenorhabditis elegans for high-resolution imaging and microinjection

Lab on a Chip ◽  
2014 ◽  
Vol 14 (18) ◽  
pp. 3498 ◽  
Author(s):  
Hyundoo Hwang ◽  
Jan Krajniak ◽  
Yohei Matsunaga ◽  
Guy M. Benian ◽  
Hang Lu
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
High Resolution Imaging ◽  
On Demand ◽  
Resolution Imaging

scholarly journals A novel microfluidic platform for high-resolution imaging of a three-dimensional cell culture under a controlled hypoxic environment

Lab on a Chip ◽  
2012 ◽  
Vol 12 (22) ◽  
pp. 4855 ◽  
Author(s):  
Kenichi Funamoto ◽  
Ioannis K. Zervantonakis ◽  
Yuchun Liu ◽  
Christopher J. Ochs ◽  
Choong Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Resolution ◽  
Three Dimensional ◽  
High Resolution Imaging ◽  
Microfluidic Platform ◽  
Hypoxic Environment ◽  
Resolution Imaging ◽  
Dimensional Cell

scholarly journals Reversible and long-term immobilization in a hydrogel-microbead matrix for high-resolution imaging of Caenorhabditis elegans and other small organisms

PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0193989 ◽  
Author(s):  
Li Dong ◽  
Matteo Cornaglia ◽  
Gopalan Krishnamani ◽  
Jingwei Zhang ◽  
Laurent Mouchiroud ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
High Resolution ◽  
High Resolution Imaging ◽  
Resolution Imaging
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