Isothermal control of probe–target interactions using graphene oxide and a nuclease for highly sensitive and specific detection of target DNA

2018 ◽  
Vol 10 (37) ◽  
pp. 4596-4603
Author(s):  
Joong Hyun Kim ◽  
Chan Ho Chung
Keyword(s):  
Graphene Oxide ◽  
Rnase H ◽  
Specific Detection ◽  
Probe Target ◽  
Target Dna ◽  
Highly Sensitive ◽  
Target Probe

Isothermal control of target–probe interaction using graphene oxide and RNase H allows highly sensitive and specific detection of target DNA.

An electrochemical impedance sensor for simple and specific recognition of G–G mismatches in DNA

2016 ◽  
Vol 8 (40) ◽  
pp. 7413-7419 ◽  
Author(s):  
Min Huang ◽  
Hanying Li ◽  
Hanping He ◽  
Xiuhua Zhang ◽  
Shengfu Wang
Keyword(s):  
Small Molecule ◽  
Gold Electrode ◽  
Electrochemical Impedance ◽  
Specific Detection ◽  
Specific Recognition ◽  
Target Dna ◽  
Highly Sensitive ◽  
Before And After ◽  
Impedance Sensor

A simple EIS sensor was developed for the highly sensitive and specific detection of G–G mismatches in dsDNA using a small molecule-modified gold electrode. Recognition and detection were achieved by ΔRct before and after incubation with target DNA.

scholarly journals Multiplex single-molecule DNA barcoding using an oligonucleotide ligation assay

2018 ◽  
Author(s):  
Ivo Severins ◽  
Malwina Szczepaniak ◽  
Chirlmin Joo
Keyword(s):  
Single Molecule ◽  
Dna Barcodes ◽  
Specific Detection ◽  
Accurate Identification ◽  
Single Nucleotide ◽  
Disease Diagnostics ◽  
Biological Studies ◽  
Target Dna ◽  
Highly Sensitive ◽  
Oligonucleotide Ligation Assay

Detection of specific nucleic acid sequences is invaluable in biological studies such as genetic disease diagnostics and genome profiling. Here we developed a highly sensitive and specific detection method that combines an advanced oligonucleotide ligation assay (OLA) with multicolor single-molecule fluorescence. We demonstrated that 7-nt long DNA barcodes have the optimal short length to ascertain specificity while being long enough for sufficient ligation. Using four spectrally separated fluorophores to label DNA barcodes, we simultaneously distinguished four DNA target sequences differing by only a single nucleotide. Our new single-molecule approach will allow for accurate identification of low abundance molecules without the need for target DNA pre-amplification.

A new mode for highly sensitive and specific detection of DNA based on exonuclease III-assisted target recycling amplification and mismatched catalytic hairpin assembly

2015 ◽  
Vol 51 (20) ◽  
pp. 4220-4222 ◽  
Author(s):  
Chenyang Tao ◽  
Yurong Yan ◽  
Hua Xiang ◽  
Dan Zhu ◽  
Wei Cheng ◽  
...  
Keyword(s):  
Dna Detection ◽  
Specific Detection ◽  
Schematic Representation ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Target Dna ◽  
Highly Sensitive ◽  
Catalytic Hairpin Assembly ◽  
Recycling Amplification

Schematic representation of the designed strategy for target DNA detection.

RNase H amplified RNA probe and graphene oxide system for highly sensitive detection of (CAG)n DNA repeat sequences

2019 ◽  
Vol 30 (46) ◽  
pp. 465502 ◽  
Author(s):  
Zhaoqi Yang ◽  
Qianxing Hu ◽  
Lan Qin ◽  
Shiyu Zhu ◽  
Lirong Qiu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Oxide System ◽  
Rnase H ◽  
Sensitive Detection ◽  
Repeat Sequences ◽  
Dna Repeat ◽  
Highly Sensitive ◽  
Rna Probe ◽  
Highly Sensitive Detection ◽  
Dna Repeat Sequences

scholarly journals Highly sensitive and specific screening of EGFR mutation using a PNA microarray-based fluorometric assay based on rolling circle amplification and graphene oxide

RSC Advances ◽  
2019 ◽  
Vol 9 (66) ◽  
pp. 38298-38308 ◽  
Author(s):  
Xiaojun Xu ◽  
Shu Xing ◽  
Mengjia Xu ◽  
Pan Fu ◽  
Tingting Gao ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Egfr Mutation ◽  
Rolling Circle Amplification ◽  
Specific Detection ◽  
Fluorometric Method ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
Detection Probe

A facile peptide nucleic acid microarray-based fluorometric method was developed for sensitive and specific detection of EGFR mutation by using rolling circle amplification, graphene oxide, and a fluorescently-labeled detection probe.

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