An electrochemical impedance sensor for simple and specific recognition of G–G mismatches in DNA

2016 ◽  
Vol 8 (40) ◽  
pp. 7413-7419 ◽  
Author(s):  
Min Huang ◽  
Hanying Li ◽  
Hanping He ◽  
Xiuhua Zhang ◽  
Shengfu Wang
Keyword(s):  
Small Molecule ◽  
Gold Electrode ◽  
Electrochemical Impedance ◽  
Specific Detection ◽  
Specific Recognition ◽  
Target Dna ◽  
Highly Sensitive ◽  
Before And After ◽  
Impedance Sensor

A simple EIS sensor was developed for the highly sensitive and specific detection of G–G mismatches in dsDNA using a small molecule-modified gold electrode. Recognition and detection were achieved by ΔRct before and after incubation with target DNA.

scholarly journals Multiplex single-molecule DNA barcoding using an oligonucleotide ligation assay

2018 ◽  
Author(s):  
Ivo Severins ◽  
Malwina Szczepaniak ◽  
Chirlmin Joo
Keyword(s):  
Single Molecule ◽  
Dna Barcodes ◽  
Specific Detection ◽  
Accurate Identification ◽  
Single Nucleotide ◽  
Disease Diagnostics ◽  
Biological Studies ◽  
Target Dna ◽  
Highly Sensitive ◽  
Oligonucleotide Ligation Assay

Detection of specific nucleic acid sequences is invaluable in biological studies such as genetic disease diagnostics and genome profiling. Here we developed a highly sensitive and specific detection method that combines an advanced oligonucleotide ligation assay (OLA) with multicolor single-molecule fluorescence. We demonstrated that 7-nt long DNA barcodes have the optimal short length to ascertain specificity while being long enough for sufficient ligation. Using four spectrally separated fluorophores to label DNA barcodes, we simultaneously distinguished four DNA target sequences differing by only a single nucleotide. Our new single-molecule approach will allow for accurate identification of low abundance molecules without the need for target DNA pre-amplification.

Isothermal control of probe–target interactions using graphene oxide and a nuclease for highly sensitive and specific detection of target DNA

2018 ◽  
Vol 10 (37) ◽  
pp. 4596-4603
Author(s):  
Joong Hyun Kim ◽  
Chan Ho Chung
Keyword(s):  
Graphene Oxide ◽  
Rnase H ◽  
Specific Detection ◽  
Probe Target ◽  
Target Dna ◽  
Highly Sensitive ◽  
Target Probe

Isothermal control of target–probe interaction using graphene oxide and RNase H allows highly sensitive and specific detection of target DNA.

A new mode for highly sensitive and specific detection of DNA based on exonuclease III-assisted target recycling amplification and mismatched catalytic hairpin assembly

2015 ◽  
Vol 51 (20) ◽  
pp. 4220-4222 ◽  
Author(s):  
Chenyang Tao ◽  
Yurong Yan ◽  
Hua Xiang ◽  
Dan Zhu ◽  
Wei Cheng ◽  
...  
Keyword(s):  
Dna Detection ◽  
Specific Detection ◽  
Schematic Representation ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Target Dna ◽  
Highly Sensitive ◽  
Catalytic Hairpin Assembly ◽  
Recycling Amplification

Schematic representation of the designed strategy for target DNA detection.

scholarly journals An Impedance Sensor in Detection of Immunoglobulin G with Interdigitated Electrodes on Flexible Substrate

2020 ◽  
Vol 10 (11) ◽  
pp. 4012 ◽  
Author(s):  
Kai Jin ◽  
Ping Zhao ◽  
Wenhui Fang ◽  
Yingjiao Zhai ◽  
Siyi Hu ◽  
...  
Keyword(s):  
Gold Electrode ◽  
Electrochemical Impedance ◽  
Flexible Substrate ◽  
Label Free ◽  
Polyethylene Naphthalate ◽  
Early Screening ◽  
Detection Range ◽  
Force Microscopy ◽  
Atomic Force ◽  
Impedance Sensor

Immunoassay plays an important role in the early screening and diagnosis of diseases. The use of electrochemical methods to realize the label-free, specific and rapid detection of antigens has attracted extensive attention from researchers. In this study, we realized the function of immunosensing and detection by lithography, the interdigitated gold electrode on the polyethylene naphthalate (PEN) membrane. Then, the gold electrode was biofunctionalized and the characterization was verified by atomic force microscopy, which was finally for the detection of mice IgG. This immunosensor has a low detection limit, with a broad linear detection range of 0.01–10 ng/mL. The results show that the electrochemical impedance sensor made of metal electrodes based on PEN flexible materials is suitable for immunoassay experiments. If this method could be proved by further studies, broad application prospects can be seen in routine immunoassays.

Multi-walled Carbon Nanotubes/Manganese Dioxide Nano- flowers-like/Polyaniline Nanowires Nanocomposite Modified Electrode: A New Platform for a Highly Sensitive Electrochemical Impedance DNA Sensor

2021 ◽  
Author(s):  
Tuan Chu ◽  
Luyen Thi Tran ◽  
Hoang Vinh Tran ◽  
Trung Tran ◽  
Nghia Trong Nguyen ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Dna Sensor ◽  
Label Free ◽  
Dna Sensors ◽  
Electrochemical Dna Sensor ◽  
Target Dna ◽  
Highly Sensitive ◽  
Polyaniline Nanowires ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Abstract We describe in this report a development of label-free electrochemical DNA sensor based on a novel nanostructured electrode of multi-walled carbon nanotubes (MWCNTs)/ nano-flowers-like manganese dioxide (MnO2)/polyaniline nanowires (PANi NWs) nanocomposite. The nanocomposite was synthesized in-situ onto an interdigitated platinum microelectrode (Pt) using a combination of chemical and electrochemical synthesis methods: chemical preparation of MWCNTs/MnO2 and electropolymerization of PANi NWs. The fabricated MWCNTs/MnO2/PANi NWs was then used to develop a label-free electrochemical DNA sensor for a specific gene of Escherichia coli (E.coli) O157:H7 detection. The MWCNTs/MnO2/PANi NWs modified Pt electrode’s surface can facilitate for probe DNA strands immobilization and, therefore the electrochemical signal of the DNA sensors has been improved. The electrochemical impedance spectroscopy (EIS) measurements were conducted to investigate the output signals generated by the specific binding of probe and target DNA sequences. Obtained results indicated that the developed electrochemical biosensor can detect the target DNA in the linear range of 5 pM to 500 nM with a low limit of detection (LOD) at 4.42 × 10 –13 M. The research results demonstrated that the MWCNTs/MnO2/PANi NWs nanocomposite-based electrochemical DNA sensor has a great potential application to the development of highly sensitive and selective electrochemical DNA sensors to detect pathogenic agents.

An electrochemical impedance sensor based on a small molecule modified Au electrode for the recognition of a trinucleotide repeat

The Analyst ◽  
2014 ◽  
Vol 139 (21) ◽  
pp. 5482-5487 ◽  
Author(s):  
Hanping He ◽  
Xiaoqian Peng ◽  
Min Huang ◽  
Gang Chang ◽  
Xiuhua Zhang ◽  
...  
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Small Molecule ◽  
Trinucleotide Repeat ◽  
Electrochemical Impedance ◽  
Trinucleotide Repeats ◽  
Au Electrode ◽  
Impedance Sensor ◽  
Modified Sensor

A small molecule modified sensor was developed for the detection of XGG trinucleotide repeats (X = C, T) by electrochemical impedance spectroscopy.

scholarly journals Fabrication of polypyrrole/Au nanoflowers modified gold electrode for highly sensitive sensing of paracetamol in pharmaceutical formulation

2021 ◽  
Vol 4 ◽  
pp. 100065
Author(s):  
Waleed Ahmed El-Said ◽  
Osama Nasr ◽  
Ahmed I.  A. Soliman ◽  
Emad A. Elshehy ◽  
Ziya Ahmad Khan ◽  
...  
Keyword(s):  
Gold Electrode ◽  
Pharmaceutical Formulation ◽  
Highly Sensitive
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