A new mode for highly sensitive and specific detection of DNA based on exonuclease III-assisted target recycling amplification and mismatched catalytic hairpin assembly

2015 ◽  
Vol 51 (20) ◽  
pp. 4220-4222 ◽  
Author(s):  
Chenyang Tao ◽  
Yurong Yan ◽  
Hua Xiang ◽  
Dan Zhu ◽  
Wei Cheng ◽  
...  
Keyword(s):  
Dna Detection ◽  
Specific Detection ◽  
Schematic Representation ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Target Dna ◽  
Highly Sensitive ◽  
Catalytic Hairpin Assembly ◽  
Recycling Amplification

Schematic representation of the designed strategy for target DNA detection.

Ultrasensitive electrochemical detection of Hg2+based on an Hg2+-triggered exonuclease III-assisted target recycling strategy

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5771-5778 ◽  
Author(s):  
Xiaolei Song ◽  
Yu Wang ◽  
Su Liu ◽  
Xue Zhang ◽  
Haiwang Wang ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Electrochemical Method ◽  
Sensitive Detection ◽  
Mercury Ions ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Recycling Amplification ◽  
Highly Sensitive Detection

An isothermal electrochemical method for the highly sensitive detection of mercury ions (Hg2+) was established based on Hg2+-triggered exonuclease III-aided target recycling amplification.

A sensitive biosensing strategy for DNA detection based on graphene oxide and T7 exonuclease assisted target recycling amplification

2013 ◽  
Vol 91 (12) ◽  
pp. 1266-1271 ◽  
Author(s):  
Hai-Bo Wang ◽  
Li-Juan Ou ◽  
Ke-Jing Huang ◽  
Xin-Ge Wen ◽  
Ling-Ling Wang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Molecular Beacon ◽  
Dna Detection ◽  
Detection Sensitivity ◽  
Target Recycling ◽  
Target Dna ◽  
Quenching Efficiency ◽  
Signal Probe ◽  
Recycling Amplification ◽  
T7 Exonuclease

A fluorescence biosensing strategy based on graphene oxide (GO) was reported for simple, rapid, sensitive, and selective DNA detection by T7 exonuclease assisted target recycling amplification. Due to the super fluorescence quenching efficiency of GO, the fluorescein amiditelabeled signal probe was firstly adsorbed onto the surface of GO and the fluorescence was quenched. Owing to its excellent selectivity for double-stranded DNA, T7 exonuclease was chosen as a signal-amplifying biocatalyst to improve the detection sensitivity. In the presence of target DNA, the signal probe could bind with target DNA and form a DNA duplex structure to trigger the digestion of the signal probe by T7 exonuclease, leading to the recycling of target DNA and the increasing of fluorescence intensity. Upon the recycling use of target DNA, this method achieved a high sensitivity towards target DNA with a detection limit of 0.3 pmol/L, which was lower than previously reported for GO-based DNA biosensors. Moreover, it does not require complex modifications of the molecular beacon and time-consuming thermal cycling procedures. Thus, the simple strategy provides a universal biosensing platform for DNA detection and it could find wide applications in DNA damage analysis and diagnostics.

Label-free DNA detection based on oligonucleotide-stabilized silver nanoclusters and exonuclease III-catalyzed target recycling amplification

2014 ◽  
Vol 6 (15) ◽  
pp. 6082-6087 ◽  
Author(s):  
Hui Ma ◽  
Wei Wei ◽  
Qian Lu ◽  
Zhixin Zhou ◽  
Henan Li ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Free Dna ◽  
Sensitivity And Selectivity ◽  
Exo Iii ◽  
Ag Ncs ◽  
Recycling Amplification

A label-free DNA biosensor with high sensitivity and selectivity is constructed by using DNA–Ag NCs and Exo III-catalyzed target recycling amplification.

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