Bacterial detection and identification from human synovial fluids on an integrated microfluidic system

The Analyst ◽  
2019 ◽  
Vol 144 (4) ◽  
pp. 1210-1222 ◽  
Author(s):  
Ting-Hang Liu ◽  
Shu-Shen Cheng ◽  
Huey-Ling You ◽  
Mel S. Lee ◽  
Gwo-Bin Lee
Keyword(s):  
Limit Of Detection ◽  
Microfluidic System ◽  
Joint Fluid ◽  
Bacterial Detection ◽  
Detection And Identification ◽  
System P ◽  
Colony Forming Units ◽  
Synovial Fluids ◽  
Human Joint

An integrated microfluidic system was developed for detecting and identifying four bacteria in human joint fluid with the limit of detection as low as 100 colony forming units (CFUs) per milliliter (or 20 CFUs per reaction).

scholarly journals Correction: Bacterial detection and identification from human synovial fluids on an integrated microfluidic system

The Analyst ◽  
2019 ◽  
Vol 144 (19) ◽  
pp. 5898-5898
Author(s):  
Ting-Hang Liu ◽  
Shu-Shen Cheng ◽  
Huey-Ling You ◽  
Mel S. Lee ◽  
Gwo-Bin Lee
Keyword(s):  
Microfluidic System ◽  
Bacterial Detection ◽  
Detection And Identification ◽  
System P ◽  
Synovial Fluids

Correction for ‘Bacterial detection and identification from human synovial fluids on an integrated microfluidic system’ by Ting-Hang Liu et al., Analyst, 2019, 144, 1210–1222.

Rapid isolation and diagnosis of live bacteria from human joint fluids by using an integrated microfluidic system

Lab on a Chip ◽  
2014 ◽  
Vol 14 (17) ◽  
pp. 3376-3384 ◽  
Author(s):  
Wen-Hsin Chang ◽  
Chih-Hung Wang ◽  
Sung-Yi Yang ◽  
Yi-Cheng Lin ◽  
Jiunn-Jong Wu ◽  
...  
Keyword(s):  
Periprosthetic Joint Infection ◽  
Joint Infection ◽  
Microfluidic System ◽  
Rapid Isolation ◽  
System P ◽  
Live Bacteria ◽  
Human Joint

An integrated microfluidic system capable of detecting live bacteria from clinical periprosthetic joint infection (PJI) samples within 55 minutes was developed in this study.

Rapid detection and typing of live bacteria from human joint fluid samples by utilizing an integrated microfluidic system

2015 ◽  
Vol 66 ◽  
pp. 148-154 ◽  
Author(s):  
Wen-Hsin Chang ◽  
Chih-Hung Wang ◽  
Chih-Lin Lin ◽  
Jiunn-Jong Wu ◽  
Mel S Lee ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Microfluidic System ◽  
Joint Fluid ◽  
Live Bacteria ◽  
Human Joint

Real-time Red Blood Cell Counting and Osmolarity Analysis using a Photoacoustic-based Microfluidic System

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Wenxiu Zhao ◽  
Haibo Yu ◽  
Yangdong Wen ◽  
Hao Luo ◽  
Boliang Jia ◽  
...  
Keyword(s):  
Blood Cell ◽  
Physical Properties ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Diagnostic Procedure ◽  
Microfluidic System ◽  
Cell Counting ◽  
Blood Samples ◽  
System P ◽  
Blood Cell Counting

Counting the number of red blood cells (RBCs) in blood samples is a common clinical diagnostic procedure, but conventional methods are unable to provide the size and other physical properties...

scholarly journals Detection of Active BoNT/C and D by EndoPep-MS Using MALDI Biotyper Instrument and Comparison with the Mouse Test Bioassay

Toxins ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 10
Author(s):  
Ilenia Drigo ◽  
Elena Tonon ◽  
Simone Pascoletti ◽  
Fabrizio Anniballi ◽  
Suzanne R. Kalb ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Animal Health ◽  
Lethal Dose ◽  
Reference Method ◽  
Mouse Bioassay ◽  
Peptide Fragments ◽  
Mass Spectrometers ◽  
Detection And Identification ◽  
Maldi Biotyper ◽  
Sensitivity Specificity

Botulinum neurotoxins (BoNTs) are among the most poisonous known biological substances, and therefore the availability of reliable, easy-to use tools for BoNT detection are important goals for food safety and human and animal health. The reference method for toxin detection and identification is the mouse bioassay (MBA). An EndoPep-MS method for BoNT differentiation has been developed based on mass spectrometry. We have validated and implemented the EndoPep-MS method on a Bruker MALDI Biotyper for the detection of BoNT/C and D serotypes. The method was extensively validated using experimentally and naturally contaminated samples comparing the results with those obtained with the MBA. Overall, the limit of detection (LoD) for both C and D toxins were less than or equal to two mouse lethal dose 50 (mLD50) per 500 µL for all tested matrices with the exception of feces spiked with BoNT/C which showed signals not-related to specific peptide fragments. Diagnostic sensitivity, specificity and positive predictive value were 100% (95% CI: 87.66–100%), 96.08% (95% CI: 86.54–99.52%), and 93.33% (95% CI: 78.25–98.20%), respectively, and accuracy was 97.47% (95% CI: 91.15–99.69%). In conclusion, the tests carried out showed that the EndoPep-MS method, initially developed using more powerful mass spectrometers, can be applied to the Bruker MALDI Biotyper instrument with excellent results including for detection of the proteolytic activity of BoNT/C, BoNT/D, BoNT/CD, and BoNT/DC toxins.

Label-free biosensors based on in situ formed and functionalized microwires in microfluidic devices

The Analyst ◽  
2015 ◽  
Vol 140 (23) ◽  
pp. 7896-7901 ◽  
Author(s):  
Yanlong Xing ◽  
Andreas Wyss ◽  
Norbert Esser ◽  
Petra S. Dittrich
Keyword(s):  
Microfluidic Devices ◽  
Microfluidic System ◽  
Label Free ◽  
System P

Label-free biosensors based on in situ formed and functionalized TTF–Au wires were developed using an integrated microfluidic system.

scholarly journals Development of a Gas-Tight Microfluidic System for Raman Sensing of Single Pulmonary Arterial Smooth Muscle Cells Under Normoxic/Hypoxic Conditions

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3238 ◽  
Author(s):  
Fenja Knoepp ◽  
Joel Wahl ◽  
Anders Andersson ◽  
Johan Borg ◽  
Norbert Weissmann ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Redox State ◽  
Microfluidic System ◽  
Hypoxic Conditions ◽  
Kv Channels ◽  
System P ◽  
Pulmonary Arterial ◽  
Arterial Smooth Muscle Cells ◽  
Pulmonary Arterial Smooth Muscle ◽  
Gas Tight

Acute hypoxia changes the redox-state of pulmonary arterial smooth muscle cells (PASMCs). This might influence the activity of redox-sensitive voltage-gated K+-channels (Kv-channels) whose inhibition initiates hypoxic pulmonary vasoconstriction (HPV). However, the molecular mechanism of how hypoxia—or the subsequent change in the cellular redox-state—inhibits Kv-channels remains elusive. For this purpose, a new multifunctional gas-tight microfluidic system was developed enabling simultaneous single-cell Raman spectroscopic studies (to sense the redox-state under normoxic/hypoxic conditions) and patch-clamp experiments (to study the Kv-channel activity). The performance of the system was tested by optically recording the O2-content and taking Raman spectra on murine PASMCs under normoxic/hypoxic conditions or in the presence of H2O2. Oxygen sensing showed that hypoxic levels in the gas-tight microfluidic system were achieved faster, more stable and significantly lower compared to a conventional open system (1.6 ± 0.2%, respectively 6.7 ± 0.7%, n = 6, p < 0.001). Raman spectra revealed that the redistribution of biomarkers (cytochromes, FeS, myoglobin and NADH) under hypoxic/normoxic conditions were improved in the gas-tight microfluidic system (p-values from 0.00% to 16.30%) compared to the open system (p-value from 0.01% to 98.42%). In conclusion, the new redox sensor holds promise for future experiments that may elucidate the role of Kv-channels during HPV.

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