Rapid isolation and diagnosis of live bacteria from human joint fluids by using an integrated microfluidic system

Lab on a Chip ◽  
2014 ◽  
Vol 14 (17) ◽  
pp. 3376-3384 ◽  
Author(s):  
Wen-Hsin Chang ◽  
Chih-Hung Wang ◽  
Sung-Yi Yang ◽  
Yi-Cheng Lin ◽  
Jiunn-Jong Wu ◽  
...  
Keyword(s):  
Periprosthetic Joint Infection ◽  
Joint Infection ◽  
Microfluidic System ◽  
Rapid Isolation ◽  
System P ◽  
Live Bacteria ◽  
Human Joint

An integrated microfluidic system capable of detecting live bacteria from clinical periprosthetic joint infection (PJI) samples within 55 minutes was developed in this study.

Bacterial detection and identification from human synovial fluids on an integrated microfluidic system

The Analyst ◽  
2019 ◽  
Vol 144 (4) ◽  
pp. 1210-1222 ◽  
Author(s):  
Ting-Hang Liu ◽  
Shu-Shen Cheng ◽  
Huey-Ling You ◽  
Mel S. Lee ◽  
Gwo-Bin Lee
Keyword(s):  
Limit Of Detection ◽  
Microfluidic System ◽  
Joint Fluid ◽  
Bacterial Detection ◽  
Detection And Identification ◽  
System P ◽  
Colony Forming Units ◽  
Synovial Fluids ◽  
Human Joint

An integrated microfluidic system was developed for detecting and identifying four bacteria in human joint fluid with the limit of detection as low as 100 colony forming units (CFUs) per milliliter (or 20 CFUs per reaction).

Rapid detection and typing of live bacteria from human joint fluid samples by utilizing an integrated microfluidic system

2015 ◽  
Vol 66 ◽  
pp. 148-154 ◽  
Author(s):  
Wen-Hsin Chang ◽  
Chih-Hung Wang ◽  
Chih-Lin Lin ◽  
Jiunn-Jong Wu ◽  
Mel S Lee ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Microfluidic System ◽  
Joint Fluid ◽  
Live Bacteria ◽  
Human Joint

scholarly journals Periprosthetic Joint Infection

2019 ◽  
Vol 2019 ◽  
pp. 1-2
Author(s):  
Bernd Fink ◽  
Konstantinos Anagnostakos ◽  
Heinz Winkler
Keyword(s):  
Periprosthetic Joint Infection ◽  
Joint Infection

Does Intra-articular Injection of the Ankle With Corticosteroids Increase the Risk of Subsequent Periprosthetic Joint Infection (PJI) Following Total Ankle Arthroplasty (TAA)? If So, How Long After a Prior Intra-articular Injection Can TAA Be Safely Performed?

2019 ◽  
Vol 40 (1_suppl) ◽  
pp. 3S-4S
Author(s):  
Ilker Uçkay ◽  
Christopher B. Hirose ◽  
Mathieu Assal
Keyword(s):  
Periprosthetic Joint Infection ◽  
Corticosteroid Injection ◽  
Joint Infection ◽  
Invasive Procedure ◽  
Total Ankle Arthroplasty ◽  
Level Of Evidence ◽  
Ankle Arthroplasty ◽  
Strong Consensus ◽  
Healthcare Associated ◽  
The Ideal

Recommendation: Every intra-articular injection of the ankle is an invasive procedure associated with potential healthcare-associated infections, including periprosthetic joint infection (PJI) following total ankle arthroplasty (TAA). Based on the limited current literature, the ideal timing for elective TAA after corticosteroid injection for the symptomatic native ankle joint is unknown. The consensus workgroup recommends that at least 3 months pass after corticosteroid injection and prior to performing TAA. Level of Evidence: Limited. Delegate Vote: Agree: 92%, Disagree: 8%, Abstain: 0% (Super Majority, Strong Consensus)

Real-time Red Blood Cell Counting and Osmolarity Analysis using a Photoacoustic-based Microfluidic System

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Wenxiu Zhao ◽  
Haibo Yu ◽  
Yangdong Wen ◽  
Hao Luo ◽  
Boliang Jia ◽  
...  
Keyword(s):  
Blood Cell ◽  
Physical Properties ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Diagnostic Procedure ◽  
Microfluidic System ◽  
Cell Counting ◽  
Blood Samples ◽  
System P ◽  
Blood Cell Counting

Counting the number of red blood cells (RBCs) in blood samples is a common clinical diagnostic procedure, but conventional methods are unable to provide the size and other physical properties...

scholarly journals Synovial Complement Factors in Patients with Periprosthetic Joint Infection after Undergoing Revision Arthroplasty of the Hip or Knee Joint

Diagnostics ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 434
Author(s):  
Frank Sebastian Fröschen ◽  
Sophia Schell ◽  
Matthias Dominik Wimmer ◽  
Gunnar Thorben Rembert Hischebeth ◽  
Hendrik Kohlhof ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Multiple Regression ◽  
Complement System ◽  
Periprosthetic Joint Infection ◽  
Joint Infection ◽  
Expression Patterns ◽  
Factor H ◽  
Low Grade ◽  
Complement Factors ◽  
Fluid Levels

The role and diagnostic value of the synovial complement system in patients with low-grade periprosthetic joint infection (PJI) are unclear. We sought to evaluate, for the first time, the usefulness of synovial complement factors in these patients by measuring the individual synovial fluid levels of complement factors (C1q, C3b/iC3b, C4b, C5, C5a, C9, factor B, factor D, factor H, factor I, properdin, and mannose-binding lectin [MBL]). The patients (n = 74) were classified into septic (n = 28) and aseptic (n = 46). Receiver-operator characteristic curves and a multiple regression model to determine the feasibility of a combination of the tested cytokines to determine the infection status were calculated. The synovial fluid levels of C1q, C3b/C3i, C4b, C5, C5a, MBL, and properdin were significantly elevated in the PJI group. The best sensitivity and specificity was found for C1q. The multiple regression models revealed that the combination of C1q, C3b/C3i, C4b, C5, C5a, and MBL was associated with the best sensitivity (83.3%) and specificity (79.2%) for a cutoff value of 0.62 (likelihood ratio: 4.0; area under the curve: 0.853). Nevertheless, only a combined model showed acceptable results. The expression patterns of the complement factors suggested that PJI activates all three pathways of the complement system.

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