Covalent functionalization of graphene oxide with d-mannose: evaluating the hemolytic effect and protein corona formation

2018 ◽  
Vol 6 (18) ◽  
pp. 2803-2812 ◽  
Author(s):  
Marcelo de Sousa ◽  
Carlos H. Z. Martins ◽  
Lidiane S. Franqui ◽  
Leandro C. Fonseca ◽  
Fabrício S. Delite ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Plasma Protein ◽  
Protein Interactions ◽  
Protein Corona ◽  
Covalent Functionalization ◽  
Corona Formation ◽  
Hemolytic Effect

Graphene oxide mannosylation impacts on RBCs toxicity and plasma protein interactions.

scholarly journals Plasma Protein Corona Reduces the Haemolytic Activity of the Graphene Oxide Nano and Micro Flakes

2016 ◽  
Vol 110 (3) ◽  
pp. 167a ◽  
Author(s):  
Marco De Spirito ◽  
Massimiliano Papi ◽  
Giuseppe Maolucci ◽  
Gabriele Ciasca ◽  
Valentina Palmieri ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Plasma Protein ◽  
Protein Corona ◽  
Haemolytic Activity

scholarly journals Poly (N-vinylpyrrolidone) modification mitigates plasma protein corona formation on phosphomolybdate-based nanoparticles

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Youyi Yu ◽  
Behafarid Ghalandari ◽  
Guangxia Shen ◽  
Liping Wang ◽  
Xiao Liu ◽  
...  
Keyword(s):  
Plasma Protein ◽  
Protein Corona ◽  
Docking Simulations ◽  
Corona Formation ◽  
Liquid Chromatography Tandem Mass ◽  
Plasma Corona ◽  
Number Of Binding Sites ◽  
Zwitterionic Polymer ◽  
Rapid Clearance ◽  
Macrophage Uptake

AbstractPhosphomolybdate-based nanoparticles (PMo12-based NPs) have been commonly applied in nanomedicine. However, upon contact with biofluids, proteins are quickly adsorbed onto the NPs surface to form a protein corona, which induces the opsonization and facilitates the rapid clearance of the NPs by macrophage uptake. Herein, we introduce a family of structurally homologous PMo12-based NPs (CDS-PMo12@PVPx(x = 0 ~ 1) NPs) capping diverse content of zwitterionic polymer poly (N-vinylpyrrolidone) (PVP) to regulate the protein corona formation on PMo12-based NPs. The fluorescence quenching data indicate that the introduction of PVP effectively reduces the number of binding sites of proteins on PMo12-based NPs. Molecular docking simulations results show that the contact surface area and binding energy of proteins to CDS-PMo12@PVP1 NPs are smaller than the CDS-PMo12@PVP0 NPs. The liquid chromatography-tandem mass spectrometry (LC–MS/MS) is further applied to analyze and quantify the compositions of the human plasma corona formation on CDS-PMo12@PVPx(x = 0 ~ 1) NPs. The number of plasma protein groups adsorption on CDS-PMo12@PVP1 NPs, compared to CDS-PMo12@PVP0 NPs, decreases from 372 to 271. In addition, 76 differentially adsorption proteins are identified between CDS-PMo12@PVP0 and CDS-PMo12@PVP1 NPs, in which apolipoprotein is up-regulated in CDS-PMo12@PVP1 NPs. The apolipoprotein adsorption onto the NPs is proposed to have dysoponic activity and enhance the circulation time of NPs. Our findings demonstrate that PVP grafting on PMo12-based NPs is a promising strategy to improve the anti-biofouling property for PMo12-based nanodrug design. Graphical Abstract

scholarly journals Plasma protein corona reduces the haemolytic activity of graphene oxide nano and micro flakes

RSC Advances ◽  
2015 ◽  
Vol 5 (99) ◽  
pp. 81638-81641 ◽  
Author(s):  
M. Papi ◽  
M. C. Lauriola ◽  
V. Palmieri ◽  
G. Ciasca ◽  
G. Maulucci ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Plasma Membrane ◽  
Plasma Protein ◽  
Plasma Proteins ◽  
Protein Corona ◽  
Haemolytic Activity ◽  
Erythrocyte Plasma Membrane

GO flakes, able to disrupt the erythrocyte plasma membrane, greatly reduce their haemolytic activity after interacting with plasma proteins. Haemolysis activity increases inversely to the GO flakes size.

scholarly journals Poly (N-vinylpyrrolidone) Modification Mitigates Plasma Protein Corona Formation on Phosphomolybdate-based Nanoparticles

2021 ◽  
Author(s):  
Youyi Yu ◽  
Behafarid Ghalandari ◽  
Guangxia Shen ◽  
Liping Wang ◽  
Xiao Liu ◽  
...  
Keyword(s):  
Plasma Protein ◽  
Protein Corona ◽  
Docking Simulations ◽  
Corona Formation ◽  
Liquid Chromatography Tandem Mass ◽  
Plasma Corona ◽  
Number Of Binding Sites ◽  
Zwitterionic Polymer ◽  
Rapid Clearance ◽  
Macrophage Uptake

Abstract Phosphomolybdate-based nanoparticles (PMo12-based NPs) have been commonly applied in nanomedicine. However, upon contact with biofluids, proteins are quickly adsorbed onto the NPs surface to form a protein corona, which induces the opsonization and facilitates the rapid clearance of the NPs by macrophage uptake. Herein, we introduce a family of structurally homologous PMo12-based NPs (CDS-PMo12@PVPx(x = 0 ~ 1) NPs) capping diverse content of zwitterionic polymer poly (N-vinylpyrrolidone) (PVP) to regulate the protein corona formation on PMo12-based NPs. The fluorescence quenching data indicate that the introduction of PVP effectively reduces the number of binding sites of proteins on PMo12-based NPs. Molecular docking simulations results show that the contact surface area and binding energy of proteins to CDS-PMo12@PVP1 NPs are smaller than the CDS-PMo12@PVP0 NPs. The liquid chromatography-tandem mass spectrometry (LC-MS/MS) is further applied to analyze and quantify the compositions of the human plasma corona formation on CDS-PMo12@PVPx(x = 0 ~ 1) NPs. The number of plasma protein groups adsorption on CDS-PMo12@PVP1 NPs, compared to CDS-PMo12@PVP0 NPs, decreases from 372 to 271. In addition, 76 differentially adsorption proteins are identified between CDS-PMo12@PVP0 and CDS-PMo12@PVP1 NPs, in which apolipoprotein is up-regulated in CDS-PMo12@PVP1 NPs. The apolipoprotein adsorption onto the NPs is proposed to have dysoponic activity and enhance the circulation time of NPs. Our findings demonstrate that PVP grafting on PMo12-based NPs is a promising strategy to improve the anti-biofouling property for PMo12-based nanodrug design.

scholarly journals Protein corona – from molecular adsorption to physiological complexity

2015 ◽  
Vol 6 ◽  
pp. 857-873 ◽  
Author(s):  
Lennart Treuel ◽  
Dominic Docter ◽  
Michael Maskos ◽  
Roland H Stauber
Keyword(s):  
Protein Interactions ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Recent Advances ◽  
Corona Formation ◽  
Protein Properties ◽  
Coulomb Type ◽  
First Time ◽  
Physiological Complexity

In biological environments, nanoparticles are enshrouded by a layer of biomolecules, predominantly proteins, mediating its subsequent interactions with cells. Detecting this protein corona, understanding its formation with regards to nanoparticle (NP) and protein properties, and elucidating its biological implications were central aims of bio-related nano-research throughout the past years. Here, we discuss the mechanistic parameters that are involved in the protein corona formation and the consequences of this corona formation for both, the particle, and the protein. We review consequences of corona formation for colloidal stability and discuss the role of functional groups and NP surface functionalities in shaping NP–protein interactions. We also elaborate the recent advances demonstrating the strong involvement of Coulomb-type interactions between NPs and charged patches on the protein surface. Moreover, we discuss novel aspects related to the complexity of the protein corona forming under physiological conditions in full serum. Specifically, we address the relation between particle size and corona composition and the latest findings that help to shed light on temporal evolution of the full serum corona for the first time. Finally, we discuss the most recent advances regarding the molecular-scale mechanistic role of the protein corona in cellular uptake of NPs.

Component-Specific Analysis of Plasma Protein Corona Formation on Gold Nanoparticles Using Multiplexed Surface Plasmon Resonance

Small ◽  
2015 ◽  
Vol 12 (9) ◽  
pp. 1174-1182 ◽  
Author(s):  
Abhijeet Patra ◽  
Tao Ding ◽  
Gokce Engudar ◽  
Yi Wang ◽  
Michal Marcin Dykas ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasma Protein ◽  
Plasmon Resonance ◽  
Protein Corona ◽  
Specific Analysis ◽  
Corona Formation

Monitoring the Hemolytic Effect of Mesoporous Silica Nanoparticles after Human Blood Protein Corona Formation

2015 ◽  
Vol 2015 (27) ◽  
pp. 4595-4602 ◽  
Author(s):  
Diego Stéfani T. Martinez ◽  
Amauri J. Paula ◽  
Leandro C. Fonseca ◽  
Luis Augusto V. Luna ◽  
Camila P. Silveira ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Human Blood ◽  
Mesoporous Silica Nanoparticles ◽  
Protein Corona ◽  
Blood Protein ◽  
Corona Formation ◽  
Hemolytic Effect

Layer by layer assembled functionalized graphene oxide-based polymer brushes for superlubricity on steel-steel tribocontact

Soft Matter ◽  
2021 ◽  
Author(s):  
Suprakash Samanta ◽  
Rashmi Ranjan Sahoo
Keyword(s):  
Graphene Oxide ◽  
Nucleophilic Addition ◽  
Polymer Brushes ◽  
Addition Reaction ◽  
Layer By Layer ◽  
Functionalized Graphene ◽  
Covalent Functionalization ◽  
Functionalized Graphene Oxide ◽  
Nucleophilic Addition Reaction ◽  
Branched Polyethylenimine

Present study demonstrates a simple and multistep approach for the preparation of covalent functionalization of chemically prepared graphene oxide (GO) by branched polyethylenimine (PEI) through nucleophilic addition reaction to prepare...

Versatile Graphene Oxide Nanosheets via Covalent Functionalization and Their Applications

2021 ◽  
Author(s):  
Minju Park ◽  
Namhee Kim ◽  
Jiyoung Lee ◽  
Minsu Gu ◽  
Byeong-Su Kim
Keyword(s):  
Graphene Oxide ◽  
Two Dimensional ◽  
Graphene Oxide Nanosheets ◽  
Covalent Functionalization ◽  
Graphene Derivatives

Even though many graphene derivatives that are atomically thin two-dimensional structures, such as graphene oxide (GO), have triggered enormous interest in the scientific and industrial communities owing to their easy...

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