scholarly journals Plasma protein corona reduces the haemolytic activity of graphene oxide nano and micro flakes

RSC Advances ◽  
2015 ◽  
Vol 5 (99) ◽  
pp. 81638-81641 ◽  
Author(s):  
M. Papi ◽  
M. C. Lauriola ◽  
V. Palmieri ◽  
G. Ciasca ◽  
G. Maulucci ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Plasma Membrane ◽  
Plasma Protein ◽  
Plasma Proteins ◽  
Protein Corona ◽  
Haemolytic Activity ◽  
Erythrocyte Plasma Membrane

GO flakes, able to disrupt the erythrocyte plasma membrane, greatly reduce their haemolytic activity after interacting with plasma proteins. Haemolysis activity increases inversely to the GO flakes size.

scholarly journals Plasma Protein Corona Reduces the Haemolytic Activity of the Graphene Oxide Nano and Micro Flakes

2016 ◽  
Vol 110 (3) ◽  
pp. 167a ◽  
Author(s):  
Marco De Spirito ◽  
Massimiliano Papi ◽  
Giuseppe Maolucci ◽  
Gabriele Ciasca ◽  
Valentina Palmieri ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Plasma Protein ◽  
Protein Corona ◽  
Haemolytic Activity

Covalent functionalization of graphene oxide with d-mannose: evaluating the hemolytic effect and protein corona formation

2018 ◽  
Vol 6 (18) ◽  
pp. 2803-2812 ◽  
Author(s):  
Marcelo de Sousa ◽  
Carlos H. Z. Martins ◽  
Lidiane S. Franqui ◽  
Leandro C. Fonseca ◽  
Fabrício S. Delite ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Plasma Protein ◽  
Protein Interactions ◽  
Protein Corona ◽  
Covalent Functionalization ◽  
Corona Formation ◽  
Hemolytic Effect

Graphene oxide mannosylation impacts on RBCs toxicity and plasma protein interactions.

scholarly journals Unraveling the In Vivo Protein Corona

Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 132
Author(s):  
Johanna Simon ◽  
Gabor Kuhn ◽  
Michael Fichter ◽  
Stephan Gehring ◽  
Katharina Landfester ◽  
...  
Keyword(s):  
Plasma Proteins ◽  
Ex Vivo ◽  
Protein Corona ◽  
Blood Proteins ◽  
Therapeutic Application ◽  
Complex Situation ◽  
Ex Vivo Approach ◽  
In Vivo Administration

Understanding the behavior of nanoparticles upon contact with a physiological environment is of urgent need in order to improve their properties for a successful therapeutic application. Most commonly, the interaction of nanoparticles with plasma proteins are studied under in vitro conditions. However, this has been shown to not reflect the complex situation after in vivo administration. Therefore, here we focused on the investigation of magnetic nanoparticles with blood proteins under in vivo conditions. Importantly, we observed a radically different proteome in vivo in comparison to the in vitro situation underlining the significance of in vivo protein corona studies. Next to this, we found that the in vivo corona profile does not significantly change over time. To mimic the in vivo situation, we established an approach, which we termed “ex vivo” as it uses whole blood freshly prepared from an animal. Overall, we present a comprehensive analysis focusing on the interaction between nanoparticles and blood proteins under in vivo conditions and how to mimic this situation with our ex vivo approach. This knowledge is needed to characterize the true biological identity of nanoparticles.

scholarly journals BARTONELLA BODIES IN THE BLOOD OF A NON-SPLENECTOMIZED DOG

1935 ◽  
Vol 62 (3) ◽  
pp. 353-258 ◽  
Author(s):  
James B. McNaught ◽  
Francis M. Woods ◽  
Virgil Scott
Keyword(s):  
Intravenous Injection ◽  
Plasma Protein ◽  
Whole Blood ◽  
Protein Level ◽  
Plasma Proteins ◽  
Basal Diet ◽  
Blood Stream ◽  
Inhibitory Effect ◽  
The Many ◽  
Plasma Protein Level

A non-splenectomized dog, on a vitamin-adequate basal diet, in the course of a plasmapheresis experiment, developed an uncontrollable anemia associated with the presence of bodies in or on the erythrocytes, indistinguishable from the descriptions of Bartonella canis. The normal plasma protein level of 7.3 per cent was reduced to 4.1 per cent by diet and the removal of 5354 ml. of whole blood in 33 bleedings. The Bartonella infection was transferred to a splenectomized dog by an intravenous injection of whole blood. Each animal was apparently sterilized by one injection of neoarsphenamine equivalent to 15 mg. per kilo weight. It is possible that the spleen liberates some substance into the blood stream which has an inhibitory effect upon a latent Bartonella infection and that this protective substance was diminished by the many bleedings associated with the lowering of plasma proteins in the non-splenectomized dog and was lacking in the inoculated splenectomized dog.

scholarly journals CHANGES IN THE VOLUME OF PLASMA AND ABSOLUTE AMOUNT OF PLASMA PROTEINS IN NEPHRITIS

1924 ◽  
Vol 39 (6) ◽  
pp. 921-929 ◽  
Author(s):  
G. C. Linder ◽  
C. Lundsgaard ◽  
D. D. Van Slyke ◽  
E. Stillman
Keyword(s):  
Plasma Protein ◽  
Plasma Volume ◽  
Plasma Proteins ◽  
Protein Concentration ◽  
The Body ◽  
Absolute Amount ◽  
Low Protein

1. We have not observed gross increases in plasma volume in glomerulonephritis, nephrosis, or nephrosclerosis, even when the concentration of plasma proteins was much below normal. Our results indicate the probability that "hydremic plethora" does not occur. 2. The low protein concentration frequently observed in the plasma in nephritis is not due to increased plasma volume but to a decrease of the total amount of plasma protein in the body. 3. Changes in plasma volume showed no constant relationship to changes in edema.

scholarly journals Differential effects of insulin deprivation and systemic insulin treatment on plasma protein synthesis in type 1 diabetic people

2009 ◽  
Vol 297 (4) ◽  
pp. E889-E897 ◽  
Author(s):  
Abdul Jaleel ◽  
Katherine A. Klaus ◽  
Dawn M. Morse ◽  
Helen Karakelides ◽  
Lawrence E. Ward ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Plasma Protein ◽  
Plasma Proteins ◽  
Insulin Treatment ◽  
Two Dimensional Gel Electrophoresis ◽  
Differential Effects ◽  
Glucose Levels ◽  
Protein Gel ◽  
Plasma Insulin Levels

It remains to be determined whether systemic insulin replacement normalizes synthesis rates of different plasma proteins and whether there are differential effects on various plasma proteins. We tested a hypothesis that insulin deprivation differentially affects individual plasma protein synthesis and that systemic insulin treatment may not normalize synthesis of all plasma proteins. We measured synthesis rates of 41 plasma proteins in seven each of type 1 diabetic (T1DM) and nondiabetic participants (ND) using [ ring-13C6]phenylalanine as a tracer. T1DM were studied while on chronic insulin treatment and during 8 h insulin deprivation. Insulin treatment normalized glucose levels, but plasma insulin levels were higher during insulin treatment than during insulin deprivation in T1DM and ND. Individual plasma proteins were purified by affinity chromatography and two-dimensional gel electrophoresis. Only 41 protein gel spots from over 300 were chosen based on their protein homogeneity. Insulin deprivation and hyperglycemia either significantly increased ( n = 12) or decreased ( n = 12) synthesis rates of 24 of 41 plasma proteins in T1DM compared with ND. Insulin treatment normalized synthesis rates of 13 of these 24 proteins, which were altered during insulin deprivation. However, insulin treatment significantly altered the synthesis of 14 additional proteins. In conclusion, acute insulin deprivation caused both a decrease and increase in synthesis rates of many plasma proteins with various functions. Moreover, chronic systemic insulin treatment not only did not normalize synthesis of all plasma proteins but also altered synthesis of several additional proteins that were unaltered during insulin deprivation.

scholarly journals Plasma proteins of young African catfish (Clarias gariepinus, Burchell 1822).

1987 ◽  
Vol 35 (4) ◽  
pp. 521-524
Author(s):  
J.H. Boon ◽  
J.M. Smits ◽  
T. Wensing ◽  
E. Lo
Keyword(s):  
Health Status ◽  
Water Supply ◽  
Plasma Protein ◽  
Negative Correlation ◽  
Plasma Proteins ◽  
Clarias Gariepinus ◽  
Total Content ◽  
African Catfish ◽  
Feeding Level ◽  
Effect Of Feeding

The effect of feeding level and water supply on the total content of plasma protein (TPP) and fractions of these proteins (PPF) of young African catfish was studied. It was found that TPP can be divided into 4 fractions (PPF I-IV), of which PPF I is predominant. Analysis of the results showed a strong effect of feeding level on TPP and PPF I-IV. There was a positive correlation between TPP and the weights of PPF I-IV, and a negative correlation between PPF I and PPF II. The PPF I fraction might be usable as an indicator for the health status of young catfishes. (Abstract retrieved from CAB Abstracts by CABI’s permission)

Erythrocyte plasma membrane fluidity in avian muscular dystrophy

1979 ◽  
Vol 64 (2) ◽  
pp. 315-326 ◽  
Author(s):  
Thomas B. Eckstein ◽  
William R. Randall ◽  
Mark G. McNamee
Keyword(s):  
Plasma Membrane ◽  
Muscular Dystrophy ◽  
Membrane Fluidity ◽  
Plasma Membrane Fluidity ◽  
Erythrocyte Plasma Membrane

Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine

Nanoscale ◽  
2018 ◽  
Vol 10 (13) ◽  
pp. 6194-6204 ◽  
Author(s):  
Damir Klepac ◽  
Hana Kostková ◽  
Svetlana Petrova ◽  
Petr Chytil ◽  
Tomáš Etrych ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Human Blood ◽  
Plasma Proteins ◽  
Protein Corona ◽  
Human Blood Plasma ◽  
Blood Plasma Proteins

The classical “hard corona–soft corona” paradigm is not valid for HPMA-based nanoparticles.

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