scholarly journals Colorimetric detection of streptomycin in milk based on peroxidase-mimicking catalytic activity of gold nanoparticles

RSC Advances ◽  
2017 ◽  
Vol 7 (61) ◽  
pp. 38471-38478 ◽  
Author(s):  
Jing Zhao ◽  
Yuangen Wu ◽  
Han Tao ◽  
Huayun Chen ◽  
Wenping Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Colorimetric Detection ◽  
Colorimetric Aptasensor

A novel and effective colorimetric aptasensor based on enhanced peroxidase-mimicking catalytic ability of AuNPs was proposed for streptomycin detection.

scholarly journals Enhancement of the Peroxidase-Like Activity of Iodine-Capped Gold Nanoparticles for the Colorimetric Detection of Biothiols

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 113 ◽  
Author(s):  
Chia-Chen Chang ◽  
Tsz-Lian Hsu ◽  
Chie-Pein Chen ◽  
Chen-Yu Chen
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Detection Limit ◽  
Enzymatic Activity ◽  
Catalytic Properties ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Synergetic Effect ◽  
Vis Spectroscopy ◽  
Wide Range

A colorimetric assay was developed for the detection of biothiols, based on the peroxidase-like activity of iodine-capped gold nanoparticles (AuNPs). These AuNPs show a synergetic effect in the form of peroxidase-mimicking activity at the interface of AuNPs, while free AuNPs and iodine alone have weak catalytic properties. Thus, iodine-capped AuNPs possess good intrinsic enzymatic activity and trigger the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), leading to a change in color from colorless to yellow. When added to solution, biothiols, such as cysteine, strongly bind to the interface of AuNPs via gold-thiol bonds, inhibiting the catalytic activity of AuNPs, resulting in a decrease in oxidized TMB. Using this strategy, cysteine could be linearly determined, at a wide range of concentrations (0.5 to 20 μM), with a detection limit of 0.5 μM using UV-Vis spectroscopy. This method was applied for the detection of cysteine in diluted human urine.

scholarly journals Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2679 ◽  
Author(s):  
Zheng-Jun Xie ◽  
Xian-Yu Bao ◽  
Chi-Fang Peng
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Limit Of Detection ◽  
Low Cost ◽  
Colorimetric Detection ◽  
Colorimetric Method ◽  
Optimal Conditions ◽  
Surface Deposition ◽  
Highly Sensitive ◽  
Dna Strand

A new colorimetric detection of methylmercury (CH3Hg+) was developed, which was based on the surface deposition of Hg enhancing the catalytic activity of gold nanoparticles (AuNPs). The AuNPs were functionalized with a specific DNA strand (HT7) recognizing CH3Hg+, which was used to capture and separate CH3Hg+ by centrifugation. It was found that the CH3Hg+ reduction resulted in the deposition of Hg onto the surface of AuNPs. As a result, the catalytic activity of the AuNPs toward the chromogenic reaction of 3,3,5,5-tetramethylbenzidine (TMB)-H2O2 was remarkably enhanced. Under optimal conditions, a limit of detection of 5.0 nM was obtained for CH3Hg+ with a linear range of 10–200 nM. We demonstrated that the colorimetric method was fairly simple with a low cost and can be conveniently applied to CH3Hg+ detection in environmental samples.

Stable gold nanoparticles as a novel peroxidase mimic for colorimetric detection of cysteine

2016 ◽  
Vol 8 (11) ◽  
pp. 2494-2501 ◽  
Author(s):  
Rong Sheng Li ◽  
Hai Liu ◽  
Bin Bin Chen ◽  
Hong Zhi Zhang ◽  
Cheng Zhi Huang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Peroxidase Mimic ◽  
Sensitivity And Selectivity ◽  
Blue Solution

In this work, stable gold nanoparticles (AuNPs) were prepared with good compatibility and favorable catalytic activity. They were developed as a peroxidase mimic for colorimetric detection of cysteine with a weakened blue solution, which supplied high sensitivity and selectivity.

Ultrasensitive and selective colorimetric detection of acetamiprid pesticide based on the enhanced peroxidase-like activity of gold nanoparticles

2017 ◽  
Vol 9 (37) ◽  
pp. 5484-5493 ◽  
Author(s):  
Wenping Yang ◽  
Yuangen Wu ◽  
Han Tao ◽  
Jing Zhao ◽  
Huayun Chen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Peroxidase Activity ◽  
Colorimetric Detection ◽  
Ultrasensitive Detection ◽  
Colorimetric Aptasensor

A colorimetric aptasensor based on the peroxidase activity of AuNPs has been proposed for the rapid and ultrasensitive detection of acetamiprid.

Highly sensitive and selective colorimetric detection of Hg2+ based on the separation of Hg2+ and formation of catalytic DNA–gold nanoparticles

2016 ◽  
Vol 8 (5) ◽  
pp. 1021-1025 ◽  
Author(s):  
Chi-Fang Peng ◽  
Na Pan ◽  
Zheng-Jun Xie ◽  
Liang-Liang Wu
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Water Samples ◽  
Colorimetric Detection ◽  
Highly Sensitive ◽  
Catalytic Dna

Hg2+ ions can be absorbed onto a DNA–AuNP complex and separated from water samples while the catalytic activity of DNA–AuNPs can be promoted.

Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samples

2020 ◽  
Vol 26 (5) ◽  
pp. 430-443 ◽  
Author(s):  
Sudarat Ledlod ◽  
Supatra Areekit ◽  
Somchai Santiwatanakul ◽  
Kosum Chansiri
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Listeria Monocytogenes ◽  
Colorimetric Detection ◽  
Simultaneous Detection ◽  
Dna Aptamer ◽  
Local Market ◽  
Salmonella Spp ◽  
Meat Samples ◽  
Colorimetric Aptasensor

In this study, we successfully developed a simple and rapid method for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli using gold nanoparticles and the aptamer aptasensor. We screened 25 specific DNA aptamer candidates against these pathogens using whole-cell Systematic Evolution of Ligands by EXponential enrichment. Among them, Ap6 was selected due to its low energy minimization values of −12.25 and −27.67 kcal/mol derived from MFold and RNAFold analysis, respectively. The assay presented in this study allowed the visual colorimetric detection of labeled colloidal gold nanoparticles as well as determination of UV absorbance at 625 and 525 nm under optimized conditions. The detection limit of this aptasensor was as less as 105 CFU/ml. A random investigation of 50 meat samples, including ham and chicken sausages, collected from the local market revealed 96% accuracy, 96% specificity, and 100% sensitivity of the assay. The colorimetric aptasensor can accomplish one-step detection without pre-culture, DNA extraction, and amplification. Hence, it is an easy, rapid, specific, and qualitative assay that can be used as a point-of-care testing to directly detect multiplex foodborne pathogens.

One-pot synthesized DNA-templated Ag/Pt bimetallic nanoclusters as peroxidase mimics for colorimetric detection of thrombin

2014 ◽  
Vol 50 (86) ◽  
pp. 13103-13106 ◽  
Author(s):  
Cheng Zheng ◽  
Ai-Xian Zheng ◽  
Bo Liu ◽  
Xiao-Long Zhang ◽  
Yu He ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Colorimetric Detection ◽  
Protein Detection ◽  
High Sensitivity ◽  
One Pot ◽  
Highly Efficient ◽  
Bimetallic Nanoclusters ◽  
Peroxidase Mimics ◽  
Sensitivity And Selectivity ◽  
Colorimetric Aptasensor

One-pot synthesized DNA-templated Ag/Pt bimetallic nanoclusters possess highly-efficient peroxidase-like catalytic activity and are applied to design a colorimetric aptasensor for the protein detection with high sensitivity and selectivity.

scholarly journals Effective reduction of nitrophenols and colorimetric detection of Pb(ii) ions by Siraitia grosvenorii fruit extract capped gold nanoparticles

RSC Advances ◽  
2021 ◽  
Vol 11 (25) ◽  
pp. 15438-15448
Author(s):  
Van Thuan Le ◽  
Truong Giang Duong ◽  
Van Tan Le ◽  
Thanh Long Phan ◽  
Thi Lan Huong Nguyen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Fruit Extract ◽  
Siraitia Grosvenorii ◽  
Effective Reduction ◽  
Sensitivity And Selectivity

Siraitia grosvenorii fruit extract capped AuNPs exhibited excellent catalytic activity in the reduction of nitrophenols and high sensitivity and selectivity for detection of Pb(ii) ions.

A simple and rapid chemosensor for colorimetric detection of dimethoate pesticide based on the peroxidase-mimicking catalytic activity of gold nanoparticles

2019 ◽  
Vol 11 (41) ◽  
pp. 5337-5347 ◽  
Author(s):  
Yang Hu ◽  
Jinlong Wang ◽  
Yuangen Wu
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Rapid Detection ◽  
Colorimetric Detection ◽  
Agricultural Products

A novel chemosensor based on the inhibition of the peroxidase-like activity of AuNPs has been proposed for the simple and rapid detection of dimethoate pesticide in agricultural products.

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