Ultrasensitive and selective colorimetric detection of acetamiprid pesticide based on the enhanced peroxidase-like activity of gold nanoparticles

2017 ◽  
Vol 9 (37) ◽  
pp. 5484-5493 ◽  
Author(s):  
Wenping Yang ◽  
Yuangen Wu ◽  
Han Tao ◽  
Jing Zhao ◽  
Huayun Chen ◽  
...  

A colorimetric aptasensor based on the peroxidase activity of AuNPs has been proposed for the rapid and ultrasensitive detection of acetamiprid.

RSC Advances ◽  
2017 ◽  
Vol 7 (61) ◽  
pp. 38471-38478 ◽  
Author(s):  
Jing Zhao ◽  
Yuangen Wu ◽  
Han Tao ◽  
Huayun Chen ◽  
Wenping Yang ◽  
...  

A novel and effective colorimetric aptasensor based on enhanced peroxidase-mimicking catalytic ability of AuNPs was proposed for streptomycin detection.


2017 ◽  
Vol 9 (25) ◽  
pp. 3777-3781 ◽  
Author(s):  
Yu-Hong Cheng ◽  
Hao Tang ◽  
Jian-Hui Jiang

A novel enzyme mediated AuNP assembly based colorimetric method for ultrasensitive detection of hepatitis C virus antibody.


2020 ◽  
Vol 26 (5) ◽  
pp. 430-443 ◽  
Author(s):  
Sudarat Ledlod ◽  
Supatra Areekit ◽  
Somchai Santiwatanakul ◽  
Kosum Chansiri

In this study, we successfully developed a simple and rapid method for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli using gold nanoparticles and the aptamer aptasensor. We screened 25 specific DNA aptamer candidates against these pathogens using whole-cell Systematic Evolution of Ligands by EXponential enrichment. Among them, Ap6 was selected due to its low energy minimization values of −12.25 and −27.67 kcal/mol derived from MFold and RNAFold analysis, respectively. The assay presented in this study allowed the visual colorimetric detection of labeled colloidal gold nanoparticles as well as determination of UV absorbance at 625 and 525 nm under optimized conditions. The detection limit of this aptasensor was as less as 105 CFU/ml. A random investigation of 50 meat samples, including ham and chicken sausages, collected from the local market revealed 96% accuracy, 96% specificity, and 100% sensitivity of the assay. The colorimetric aptasensor can accomplish one-step detection without pre-culture, DNA extraction, and amplification. Hence, it is an easy, rapid, specific, and qualitative assay that can be used as a point-of-care testing to directly detect multiplex foodborne pathogens.


2018 ◽  
Vol 1020 ◽  
pp. 110-115 ◽  
Author(s):  
Khalil Abnous ◽  
Noor Mohammad Danesh ◽  
Mohammad Ramezani ◽  
Seyed Mohammad Taghdisi ◽  
Ahmad Sarreshtehdar Emrani

2021 ◽  
Author(s):  
Yiren Cao ◽  
Jinjun Wu ◽  
Bo Pang ◽  
Hongquan Zhang ◽  
X. Chris Le

The trans-cleavage activity of the target-activated CRISPR-Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and...


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