A simple and rapid chemosensor for colorimetric detection of dimethoate pesticide based on the peroxidase-mimicking catalytic activity of gold nanoparticles

2019 ◽  
Vol 11 (41) ◽  
pp. 5337-5347 ◽  
Author(s):  
Yang Hu ◽  
Jinlong Wang ◽  
Yuangen Wu
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Rapid Detection ◽  
Colorimetric Detection ◽  
Agricultural Products

A novel chemosensor based on the inhibition of the peroxidase-like activity of AuNPs has been proposed for the simple and rapid detection of dimethoate pesticide in agricultural products.

Highly efficient colorimetric detection of ATP utilizing a split aptamer target binding strategy and superior catalytic activity of graphene oxide–platinum/gold nanoparticles

RSC Advances ◽  
2015 ◽  
Vol 5 (92) ◽  
pp. 75746-75752 ◽  
Author(s):  
Siqi Zhang ◽  
Kun Wang ◽  
Jiali Li ◽  
Zhenyu Li ◽  
Ting Sun
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Catalytic Activity ◽  
Magnetic Separation ◽  
Rapid Detection ◽  
Magnetic Beads ◽  
Specific Binding ◽  
Colorimetric Detection ◽  
Colored Product ◽  
Target Binding

The specific binding of ATP and its aptamer linked the split aptamer-modified GO/PDDA/PtAuNPs and magnetic beads together. Using magnetic separation, TMB was catalyzed into a colored product by nanocomposites, which enabled rapid detection of ATP.

scholarly journals Enhancement of the Peroxidase-Like Activity of Iodine-Capped Gold Nanoparticles for the Colorimetric Detection of Biothiols

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 113 ◽  
Author(s):  
Chia-Chen Chang ◽  
Tsz-Lian Hsu ◽  
Chie-Pein Chen ◽  
Chen-Yu Chen
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Detection Limit ◽  
Enzymatic Activity ◽  
Catalytic Properties ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Synergetic Effect ◽  
Vis Spectroscopy ◽  
Wide Range

A colorimetric assay was developed for the detection of biothiols, based on the peroxidase-like activity of iodine-capped gold nanoparticles (AuNPs). These AuNPs show a synergetic effect in the form of peroxidase-mimicking activity at the interface of AuNPs, while free AuNPs and iodine alone have weak catalytic properties. Thus, iodine-capped AuNPs possess good intrinsic enzymatic activity and trigger the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), leading to a change in color from colorless to yellow. When added to solution, biothiols, such as cysteine, strongly bind to the interface of AuNPs via gold-thiol bonds, inhibiting the catalytic activity of AuNPs, resulting in a decrease in oxidized TMB. Using this strategy, cysteine could be linearly determined, at a wide range of concentrations (0.5 to 20 μM), with a detection limit of 0.5 μM using UV-Vis spectroscopy. This method was applied for the detection of cysteine in diluted human urine.

scholarly journals Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2679 ◽  
Author(s):  
Zheng-Jun Xie ◽  
Xian-Yu Bao ◽  
Chi-Fang Peng
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Limit Of Detection ◽  
Low Cost ◽  
Colorimetric Detection ◽  
Colorimetric Method ◽  
Optimal Conditions ◽  
Surface Deposition ◽  
Highly Sensitive ◽  
Dna Strand

A new colorimetric detection of methylmercury (CH3Hg+) was developed, which was based on the surface deposition of Hg enhancing the catalytic activity of gold nanoparticles (AuNPs). The AuNPs were functionalized with a specific DNA strand (HT7) recognizing CH3Hg+, which was used to capture and separate CH3Hg+ by centrifugation. It was found that the CH3Hg+ reduction resulted in the deposition of Hg onto the surface of AuNPs. As a result, the catalytic activity of the AuNPs toward the chromogenic reaction of 3,3,5,5-tetramethylbenzidine (TMB)-H2O2 was remarkably enhanced. Under optimal conditions, a limit of detection of 5.0 nM was obtained for CH3Hg+ with a linear range of 10–200 nM. We demonstrated that the colorimetric method was fairly simple with a low cost and can be conveniently applied to CH3Hg+ detection in environmental samples.

Stable gold nanoparticles as a novel peroxidase mimic for colorimetric detection of cysteine

2016 ◽  
Vol 8 (11) ◽  
pp. 2494-2501 ◽  
Author(s):  
Rong Sheng Li ◽  
Hai Liu ◽  
Bin Bin Chen ◽  
Hong Zhi Zhang ◽  
Cheng Zhi Huang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Peroxidase Mimic ◽  
Sensitivity And Selectivity ◽  
Blue Solution

In this work, stable gold nanoparticles (AuNPs) were prepared with good compatibility and favorable catalytic activity. They were developed as a peroxidase mimic for colorimetric detection of cysteine with a weakened blue solution, which supplied high sensitivity and selectivity.

scholarly journals Colorimetric detection of streptomycin in milk based on peroxidase-mimicking catalytic activity of gold nanoparticles

RSC Advances ◽  
2017 ◽  
Vol 7 (61) ◽  
pp. 38471-38478 ◽  
Author(s):  
Jing Zhao ◽  
Yuangen Wu ◽  
Han Tao ◽  
Huayun Chen ◽  
Wenping Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Colorimetric Detection ◽  
Colorimetric Aptasensor

A novel and effective colorimetric aptasensor based on enhanced peroxidase-mimicking catalytic ability of AuNPs was proposed for streptomycin detection.

Highly sensitive and selective colorimetric detection of Hg2+ based on the separation of Hg2+ and formation of catalytic DNA–gold nanoparticles

2016 ◽  
Vol 8 (5) ◽  
pp. 1021-1025 ◽  
Author(s):  
Chi-Fang Peng ◽  
Na Pan ◽  
Zheng-Jun Xie ◽  
Liang-Liang Wu
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Water Samples ◽  
Colorimetric Detection ◽  
Highly Sensitive ◽  
Catalytic Dna

Hg2+ ions can be absorbed onto a DNA–AuNP complex and separated from water samples while the catalytic activity of DNA–AuNPs can be promoted.

scholarly journals Effective reduction of nitrophenols and colorimetric detection of Pb(ii) ions by Siraitia grosvenorii fruit extract capped gold nanoparticles

RSC Advances ◽  
2021 ◽  
Vol 11 (25) ◽  
pp. 15438-15448
Author(s):  
Van Thuan Le ◽  
Truong Giang Duong ◽  
Van Tan Le ◽  
Thanh Long Phan ◽  
Thi Lan Huong Nguyen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Fruit Extract ◽  
Siraitia Grosvenorii ◽  
Effective Reduction ◽  
Sensitivity And Selectivity

Siraitia grosvenorii fruit extract capped AuNPs exhibited excellent catalytic activity in the reduction of nitrophenols and high sensitivity and selectivity for detection of Pb(ii) ions.

Directed Evolution of P450 Fatty Acid Decarboxylases via High-Throughput Screening Towards Improved Catalytic Activity

2019 ◽  
Author(s):  
Huifang Xu ◽  
Weinan Liang ◽  
Linlin Ning ◽  
Yuanyuan Jiang ◽  
Wenxia Yang ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Fatty Acid ◽  
Directed Evolution ◽  
High Throughput ◽  
High Throughput Screening ◽  
Colorimetric Detection ◽  
Screening Method ◽  
Random Mutagenesis ◽  
Direct Production ◽  
Consumption Activity

P450 fatty acid decarboxylases (FADCs) have recently been attracting considerable attention owing to their one-step direct production of industrially important 1-alkenes from biologically abundant feedstock free fatty acids under mild conditions. However, attempts to improve the catalytic activity of FADCs have met with little success. Protein engineering has been limited to selected residues and small mutant libraries due to lack of an effective high-throughput screening (HTS) method. Here, we devise a catalase-deficient <i>Escherichia coli</i> host strain and report an HTS approach based on colorimetric detection of H<sub>2</sub>O<sub>2</sub>-consumption activity of FADCs. Directed evolution enabled by this method has led to effective identification for the first time of improved FADC variants for medium-chain 1-alkene production from both DNA shuffling and random mutagenesis libraries. Advantageously, this screening method can be extended to other enzymes that stoichiometrically utilize H<sub>2</sub>O<sub>2</sub> as co-substrate.

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