Microfluidic bead trap as a visual bar for quantitative detection of oligonucleotides

Lab on a Chip ◽  
2017 ◽  
Vol 17 (19) ◽  
pp. 3240-3245 ◽  
Author(s):  
Zichen Zhao ◽  
Yuanye Bao ◽  
Lok Ting Chu ◽  
John Kin Lim Ho ◽  
Ching-Chang Chieng ◽  
...  
Keyword(s):  
Quantitative Detection ◽  
Naked Eye

We demonstrate a microfluidic bead trap capable of forming a dipstick-type bar visible to the naked eye for simple and quantitative detection of oligonucleotides.

Bio-inspired photonic crystals for naked eye quantification of nucleic acids

The Analyst ◽  
2019 ◽  
Vol 144 (18) ◽  
pp. 5413-5419 ◽  
Author(s):  
Ke Zhu ◽  
Junjie Chi ◽  
Dagan Zhang ◽  
Biao Ma ◽  
Xing Dong ◽  
...  
Keyword(s):  
Photonic Crystals ◽  
Nucleic Acids ◽  
Structural Color ◽  
Quantitative Detection ◽  
Naked Eye

The quantitative detection of nucleic acids by the naked eye was achieved based on structural color.

Quantitative Detection of Whey Cream in Sweet Cream and Butter

1994 ◽  
Vol 77 (2) ◽  
pp. 488-490 ◽  
Author(s):  
Pavel Polak ◽  
Robert L Bradley
Keyword(s):  
Sialic Acid ◽  
Calibration Curve ◽  
Seasonal Variability ◽  
Color Reaction ◽  
Cow’S Milk ◽  
Quantitative Detection ◽  
Sensitive Assay ◽  
Cow's Milk ◽  
Naked Eye ◽  
Purple Color

Abstract A simple and sensitive assay is described that can detect trace amounts of whey cream in sweet cream and in butter made from that cream. The method is based on the detection of sialic acid and involves a color reaction between Ehrlich’s reagent and sialic acid. The procedure is faster than other methods for detection of whey. The method could be termed semiquantitative because of the seasonal variability of sialic acid in cow’s milk. However, with a calibration curve, the method is quantitative and can easily detect as little as 1% whey cream in sweet cream and in the resulting butter. With 1% whey cream in sweet cream, the purple color indicating the presence of sialic acid is visible to the naked eye.

Semi-quantitative detection of p-Aminophenol in real samples with colorfully naked-eye assay

2021 ◽  
Vol 334 ◽  
pp. 129604
Author(s):  
Ming Li ◽  
Caiping Ding ◽  
Pengde Jia ◽  
Longhua Guo ◽  
Sui Wang ◽  
...  
Keyword(s):  
Quantitative Detection ◽  
Real Samples ◽  
Naked Eye

scholarly journals Evaluation of Fluorescent Cu2+ Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 512
Author(s):  
Hao He ◽  
Zhao Cheng ◽  
Lei Zheng ◽  
Xuejiao Zhang
Keyword(s):  
Cell Imaging ◽  
Rhodamine 6G ◽  
Detection Method ◽  
Quantitative Detection ◽  
Naked Eye ◽  
Optical Stability ◽  
Low Toxicity ◽  
Real Time Tracking ◽  
Opening And Closing ◽  
Ecv304 Cell

By incorporating a rhodamine spirolactam structure as the recognition site for Cu2+, two novel probes were synthesized through a connection of rhodamine 6G acylhydrazine and 5-formyl-6-hydroxyl-4-methylcoumarin/2,4-dihydroxybenzaldehyde. In the recognition process of probes towards Cu2+, the spirolactam ring exhibited opening and closing, accompanying an instant and specific change in fluorescence and in color, which could also achieve a naked-eye and semiquantitative recognition of aqueous Cu2+ besides the fluorescent Cu2+ detection method. Fluorescent analyses and ECV304 cell imaging further revealed the probes’ good optical stability, instant response, low toxicity, and membrane permeability, which offers future possibilities for the probes’ instant detection and the real-time tracking of Cu2+ in biological systems.

Tb-MOF: a naked-eye and regenerable fluorescent probe for selective and quantitative detection of Fe3+and Al3+ions

RSC Advances ◽  
2016 ◽  
Vol 6 (97) ◽  
pp. 94622-94628 ◽  
Author(s):  
Mengfei Zhang ◽  
Jing Han ◽  
Haipeng Wu ◽  
Qing Wei ◽  
Gang Xie ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
High Selectivity ◽  
Quantitative Detection ◽  
Naked Eye

A unique Tb-MOF fluorescent probe has features that are visible to the naked-eye and can be regenerated; it presents high selectivity and sensitivity to the quantitative detection of Fe3+and Al3+ions.

scholarly journals Instrument-free quantitative gold nanoparticle-based liquid-phase colorimetric assays for use in resource-poor environments

2017 ◽  
Vol 53 (60) ◽  
pp. 8407-8410 ◽  
Author(s):  
Huili Wang ◽  
Yun Zhang ◽  
Rongxing Li ◽  
Jinfang Nie ◽  
Afaf H. El-Sagheer ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Gold Nanoparticle ◽  
Colorimetric Assay ◽  
Quantitative Detection ◽  
Two Dimensional ◽  
New Class ◽  
Naked Eye ◽  
Resource Poor ◽  
Colorimetric Assays

This work describes a new class of gold nanoparticle-based liquid-phase colorimetric assay (GNP-LPCA) termed as two dimensional (2D) GNP-LPCA that enables quantitative detection of model analytes with naked eye.

scholarly journals Naked eye evaluation and quantitative detection of the sugarcane leaf scald pathogen, Xanthomonas albilineans, in sugarcane xylem sap

2021 ◽  
Author(s):  
Muhammad Umer ◽  
Nahian Binte Aziz ◽  
Salma Al Jabri ◽  
Shamsul A. Bhuiyan ◽  
Muhammad J. A. Shiddiky
Keyword(s):  
Xylem Sap ◽  
Quantitative Detection ◽  
Leaf Scald ◽  
Naked Eye ◽  
Xanthomonas Albilineans

New two dimensional liquid-phase colorimetric assay based on old iodine–starch complexation for the naked-eye quantitative detection of analytes

2016 ◽  
Vol 52 (47) ◽  
pp. 7454-7457 ◽  
Author(s):  
Jinfang Nie ◽  
Tom Brown ◽  
Yun Zhang
Keyword(s):  
Liquid Phase ◽  
Colorimetric Assay ◽  
Point Of Care ◽  
Low Cost ◽  
Quantitative Detection ◽  
Complexation Reaction ◽  
Two Dimensional ◽  
Eye Detection ◽  
Naked Eye ◽  
Naked Eye Detection

This work describes a new point-of-care two dimensional liquid-phase colorimetric assay (2D LPCA) based on old iodine–starch complexation reaction firstly discovered in 1814 for simple, low-cost, portable, naked-eye detection and quantification of two model analytes.

Therapeutic efficacy of some extracts of hot pepper fruits (Capsicum annuum) and Sumac seeds (Rhus coriaria) on the growth of some isolated dermatophytes species

2018 ◽  
Vol 1 (3) ◽  
pp. 52-62
Author(s):  
Sara Omran ◽  
Abdulghani Alsamarai ◽  
Firas Razzzaq
Keyword(s):  
Antifungal Activity ◽  
Fungal Infection ◽  
Aqueous Extract ◽  
Plant Extracts ◽  
Therapeutic Efficacy ◽  
Diffusion Method ◽  
Quantitative Detection ◽  
Hot Pepper ◽  
Alcoholic Extract ◽  
Dermatology Clinic

Background: Fungal infections are one of the common skin diseases with difficulty in their treatment approach. The present efficient drugs for fungal infection are limited. Aim: To determine the therapeutic efficacy of plant extracts as alternative antifungal agents. Materials and methods: 100 clinical samples [68 from female and 32 from male] were collected during the period from March to July 2017 from subjects attending Dermatology Clinic in Salah Uldean General Hospital. Fungal infection was diagnosed with using KOH wet preparation. Fungal species identified by using conventional approach. The active ingredients existing in the plant extracts were detected and analyzed through qualitative and quantitative detection technique of chemical compounds using a high performance liquid chromatographic device (HPLC). Agar diffusion method was used to determine antifungal activity of plant extracts. Results: Direct microscopic examination showed that there were (75%) positive samples, while culture shows (67%) positive samples. The isolated dermatophytes belong to Epidermophyoton, Microsporum, and Trichophyton genus. The predominant dermatophytes were T. rubrum (25%) species. The highest frequency of infection was in the age group of 11-20 years. The sensitivity of the tested fungi to the aqueous and alcoholic plant extracts varies. Alcoholic extract of the hot pepper plant was more effective as antifungal than the aqueous extract of the same plant. However, aqueous hot pepper extracts was more effective against T. mentagrophyte than that of alcoholic extract. Additionally, alcoholic Sumac extract shows higher efficacy that aqueous extract. Conclusion: Hot pepper and Sumac extracts show antifungal activity against Microsporum canis, Trichophyton rubrum and T. mentagrophyte.

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