scholarly journals Evaluation of Fluorescent Cu2+ Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 512
Author(s):  
Hao He ◽  
Zhao Cheng ◽  
Lei Zheng ◽  
Xuejiao Zhang
Keyword(s):  
Cell Imaging ◽  
Rhodamine 6G ◽  
Detection Method ◽  
Quantitative Detection ◽  
Naked Eye ◽  
Optical Stability ◽  
Low Toxicity ◽  
Real Time Tracking ◽  
Opening And Closing ◽  
Ecv304 Cell

By incorporating a rhodamine spirolactam structure as the recognition site for Cu2+, two novel probes were synthesized through a connection of rhodamine 6G acylhydrazine and 5-formyl-6-hydroxyl-4-methylcoumarin/2,4-dihydroxybenzaldehyde. In the recognition process of probes towards Cu2+, the spirolactam ring exhibited opening and closing, accompanying an instant and specific change in fluorescence and in color, which could also achieve a naked-eye and semiquantitative recognition of aqueous Cu2+ besides the fluorescent Cu2+ detection method. Fluorescent analyses and ECV304 cell imaging further revealed the probes’ good optical stability, instant response, low toxicity, and membrane permeability, which offers future possibilities for the probes’ instant detection and the real-time tracking of Cu2+ in biological systems.

scholarly journals A pH tuning single fluorescent probe based on naphthalene for dual-analytes (Mg2+ and Al3+) and its application in cell imaging

RSC Advances ◽  
2020 ◽  
Vol 10 (36) ◽  
pp. 21399-21405
Author(s):  
Chunwei Yu ◽  
Shuhua Cui ◽  
Yuxiang Ji ◽  
Shaobai Wen ◽  
Li Jian ◽  
...  
Keyword(s):  
Schiff Base ◽  
Fluorescent Probe ◽  
Cell Imaging ◽  
Quantitative Detection

In this study, a naphthalene Schiff-base P which serves as a dual-analyte probe for the quantitative detection of Al3+ and Mg2+ has been designed.

Microfluidic bead trap as a visual bar for quantitative detection of oligonucleotides

Lab on a Chip ◽  
2017 ◽  
Vol 17 (19) ◽  
pp. 3240-3245 ◽  
Author(s):  
Zichen Zhao ◽  
Yuanye Bao ◽  
Lok Ting Chu ◽  
John Kin Lim Ho ◽  
Ching-Chang Chieng ◽  
...  
Keyword(s):  
Quantitative Detection ◽  
Naked Eye

We demonstrate a microfluidic bead trap capable of forming a dipstick-type bar visible to the naked eye for simple and quantitative detection of oligonucleotides.

Green and Simple Synthesis of Photoluminescence-Tunable Carbon Dots for Sensing and Cell Imaging Applications

2021 ◽  
Vol 21 (12) ◽  
pp. 6101-6110
Author(s):  
Dong Sun ◽  
Shu-Jun Li ◽  
Chun-Feng Wang ◽  
Tian-Tian Liu ◽  
Guang-Yue Bai ◽  
...  
Keyword(s):  
Boric Acid ◽  
Carbon Dots ◽  
Luminescence Property ◽  
Cell Imaging ◽  
Fluorescence Emission ◽  
Fluorescent Imaging ◽  
Simple Synthesis ◽  
Low Toxicity ◽  
Doped Carbon Dots ◽  
Co Doped

Innovative nitrogen and boron co-doped carbon dots are hydrothermally produced using fructose, urea, and boric acid as precursors. The synthesized carbon dots possess a uniform morphology, and exhibit excellent fluorescence stability, tunable luminescence property, strong resistance to photobleaching, low-toxicity, and excellent biocompatibility. It is also found more dopant urea is conducive to the formation of the carbon dots with more B–N bonds, and shorter wavelength of fluorescence emission. Meanwhile, the synthesized carbon dots are well utilized as a photoluminescent probe for facile Hg2+ determination and fluorescent imaging reagent in cells.

scholarly journals A general fluorescent light-up probe for staining and quantifying protein

2019 ◽  
Vol 6 (8) ◽  
pp. 190580 ◽  
Author(s):  
Jiawei Zou ◽  
Gangyi Chen ◽  
Feng Du ◽  
Yi Yuan ◽  
Xin Huang ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Visual Analysis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Fluorescent Light ◽  
Cell Penetration ◽  
Cellular Processes ◽  
Low Toxicity ◽  
Protein Rich

Proteins are the primary functional agents in all cellular processes, facilitating various functions such as enzymes and structure-forming or signal-transducing molecules. In this work, we report a fluorescent dye, PyMDI-Zn, which could specifically bind with proteins and provide a red-shifted fluorescent emission. The visual analysis of protein in sodium dodecyl sulfate-polyacrylamide gel electrophoresis could be realized in 5 min by using PyMDI-Zn as a light-up dye. Based on its cell penetration and low toxicity, PyMDI-Zn could also be applied to locate protein-rich regions and organelles in live cell imaging. Moreover, the direct protein quantitation can be realized based on PyMDI-Zn, providing a method of screening for food adulteration by nitrogen-rich compounds.

Bio-inspired photonic crystals for naked eye quantification of nucleic acids

The Analyst ◽  
2019 ◽  
Vol 144 (18) ◽  
pp. 5413-5419 ◽  
Author(s):  
Ke Zhu ◽  
Junjie Chi ◽  
Dagan Zhang ◽  
Biao Ma ◽  
Xing Dong ◽  
...  
Keyword(s):  
Photonic Crystals ◽  
Nucleic Acids ◽  
Structural Color ◽  
Quantitative Detection ◽  
Naked Eye

The quantitative detection of nucleic acids by the naked eye was achieved based on structural color.

A rapid and naked-eye visible rhodamine 6G-based chemosensor for sensitive detection of copper(ii) ions in aqueous solution

2018 ◽  
Vol 10 (47) ◽  
pp. 5731-5737 ◽  
Author(s):  
Q. Huang ◽  
Y. T. Chen ◽  
Y. W. Ren ◽  
Z. Y. Wang ◽  
Y. X. Zhu ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Aqueous Solutions ◽  
High Selectivity ◽  
Rhodamine 6G ◽  
Sensitive Detection ◽  
Naked Eye ◽  
Eye Recognition

The chemosensor R6GHP showed high selectivity for Cu2+ with “naked-eye” recognition in aqueous solutions.

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