scholarly journals Naked eye evaluation and quantitative detection of the sugarcane leaf scald pathogen, Xanthomonas albilineans, in sugarcane xylem sap

2021 ◽  
Author(s):  
Muhammad Umer ◽  
Nahian Binte Aziz ◽  
Salma Al Jabri ◽  
Shamsul A. Bhuiyan ◽  
Muhammad J. A. Shiddiky
Keyword(s):  
Xylem Sap ◽  
Quantitative Detection ◽  
Leaf Scald ◽  
Naked Eye ◽  
Xanthomonas Albilineans

Microfluidic bead trap as a visual bar for quantitative detection of oligonucleotides

Lab on a Chip ◽  
2017 ◽  
Vol 17 (19) ◽  
pp. 3240-3245 ◽  
Author(s):  
Zichen Zhao ◽  
Yuanye Bao ◽  
Lok Ting Chu ◽  
John Kin Lim Ho ◽  
Ching-Chang Chieng ◽  
...  
Keyword(s):  
Quantitative Detection ◽  
Naked Eye

We demonstrate a microfluidic bead trap capable of forming a dipstick-type bar visible to the naked eye for simple and quantitative detection of oligonucleotides.

Bio-inspired photonic crystals for naked eye quantification of nucleic acids

The Analyst ◽  
2019 ◽  
Vol 144 (18) ◽  
pp. 5413-5419 ◽  
Author(s):  
Ke Zhu ◽  
Junjie Chi ◽  
Dagan Zhang ◽  
Biao Ma ◽  
Xing Dong ◽  
...  
Keyword(s):  
Photonic Crystals ◽  
Nucleic Acids ◽  
Structural Color ◽  
Quantitative Detection ◽  
Naked Eye

The quantitative detection of nucleic acids by the naked eye was achieved based on structural color.

scholarly journals Identification of Differentially Expressed Proteins in Sugarcane in Response to Infection by Xanthomonas albilineans Using iTRAQ Quantitative Proteomics

2020 ◽  
Vol 8 (1) ◽  
pp. 76
Author(s):  
Jian-Yu Meng ◽  
Mbuya Sylvain Ntambo ◽  
Philippe C. Rott ◽  
Hua-Ying Fu ◽  
Mei-Ting Huang ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Vascular System ◽  
Lipid Transfer Protein ◽  
Differentially Expressed ◽  
Post Inoculation ◽  
Differentially Expressed Proteins ◽  
Lipid Transfer ◽  
Leaf Scald ◽  
Improvement Programs ◽  
Xanthomonas Albilineans

Sugarcane can suffer severe yield losses when affected by leaf scald, a disease caused by Xanthomonas albilineans. This bacterial pathogen colonizes the vascular system of sugarcane, which can result in reduced plant growth and plant death. In order to better understand the molecular mechanisms involved in the resistance of sugarcane to leaf scald, a comparative proteomic study was performed with two sugarcane cultivars inoculated with X. albilineans: one resistant (LCP 85-384) and one susceptible (ROC20) to leaf scald. The iTRAQ (isobaric tags for relative and absolute quantification) approach at 0 and 48 h post-inoculation (hpi) was used to identify and annotate differentially expressed proteins (DEPs). A total of 4295 proteins were associated with 1099 gene ontology (GO) terms by GO analysis. Among those, 285 were DEPs during X. albilineans infection in cultivars LCP 85-384 and ROC20. One hundred seventy-two DEPs were identified in resistant cultivar LCP 85-384, and 113 of these proteins were upregulated and 59 were downregulated. One hundred ninety-two DEPs were found in susceptible cultivar ROC20 and half of these (92) were upregulated, whereas the other half corresponded to downregulated proteins. The significantly upregulated DEPs in LCP 85-384 were involved in metabolic pathways, the biosynthesis of secondary metabolites, and the phenylpropanoid biosynthesis pathway. Additionally, the expression of seven candidate genes related to photosynthesis and glycolytic pathways, plant innate immune system, glycosylation process, plant cytochrome P450, and non-specific lipid transfer protein was verified based on transcription levels in sugarcane during infection by X. albilineans. Our findings shed new light on the differential expression of proteins in sugarcane cultivars in response to infection by X. albilineans. The identification of these genes provides important information for sugarcane variety improvement programs using molecular breeding strategies.

Quantitative Detection of Whey Cream in Sweet Cream and Butter

1994 ◽  
Vol 77 (2) ◽  
pp. 488-490 ◽  
Author(s):  
Pavel Polak ◽  
Robert L Bradley
Keyword(s):  
Sialic Acid ◽  
Calibration Curve ◽  
Seasonal Variability ◽  
Color Reaction ◽  
Cow’S Milk ◽  
Quantitative Detection ◽  
Sensitive Assay ◽  
Cow's Milk ◽  
Naked Eye ◽  
Purple Color

Abstract A simple and sensitive assay is described that can detect trace amounts of whey cream in sweet cream and in butter made from that cream. The method is based on the detection of sialic acid and involves a color reaction between Ehrlich’s reagent and sialic acid. The procedure is faster than other methods for detection of whey. The method could be termed semiquantitative because of the seasonal variability of sialic acid in cow’s milk. However, with a calibration curve, the method is quantitative and can easily detect as little as 1% whey cream in sweet cream and in the resulting butter. With 1% whey cream in sweet cream, the purple color indicating the presence of sialic acid is visible to the naked eye.

scholarly journals PENGARUH METODE STERILISASI DAN KONSENTRASI FILTRAT BAKTERI ENDOFIT TANAMAN TEBU UNTUK MENGENDALIKAN PENYAKIT BLENDOK (Xanthomonas albilineans (Ashby) Savulescu 1947)

2017 ◽  
Vol 4 (1) ◽  
pp. 20-39
Author(s):  
Astri Afriani
Keyword(s):  
Endophytic Bacteria ◽  
Membrane Filter ◽  
Petri Dish ◽  
Hot Water ◽  
Control Measures ◽  
Leaf Scald ◽  
Randomized Design ◽  
Cutting Knife ◽  
North Sumatra ◽  
Xanthomonas Albilineans

Leaf scald (Xanthomonas albilineans) is an important pathogen of sugar cane in Indonesiaand cause significant losses at several main sugarcane plantations. Some control measures, e.i.bactericides smeared Lysol 20% or alcohol 70% on cutting knife seedling for disinfectant,and hot water treatment at 50 minutes on 52,5ᵒC, have not given satisfactory results inmanaging this leaf scald in sugarcane on the field. Biological control with filtrate ofendophytes bacterial were alternative to biocontrol agents Xanthomonas albilineans. Theobjectives of this research were to determine: influence of sterilization methods andendophytic bacterial filtrate dosages of sugarcane to control leaf scald (Xanthomonasalbilineans). This research was conducted in Phytopathology Laboratory at the Faculty ofAgriculture University of North Sumatra from April until December 2014. This research haddone with Completely Randomized Design (CRD) with three-factor: 1. type of endophyticbacterial filtrates: filtrate of B2, B7 and B9 Sterilization methods of endophytic bacterialfiltrate: control (no sterilization), Sterilization by autoclave and Sterilization by filtermembrane, 3. Dosage of endophytic filtrate with four-level: 0,1%, 0,01%, 0,001% and0,0001%. The result showed: 10 isolate endophytic bacteria had exploration were: B1, B2,B3, B4, B5, B6, B7, B8, B9, and B10. Three isolates had a clear zone on Petri dish ≥ 20 mm invitro testing is B9, B7, and B2. The results showed that the membrane filter sterilizationmethod was better when compared to autoclave sterilization, namely: the highest inhibitoryzone diameter of endophytic bacteria in the treatment of B9 filtrate with a membrane filtersterilization method 0,1% at 3 days after inoculation that was equal to 23.00 mm.

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