A non-enzyme cascade amplification strategy for colorimetric assay of disease biomarkers

2017 ◽  
Vol 53 (65) ◽  
pp. 9055-9058 ◽  
Author(s):  
Jiuxing Li ◽  
Zhuangqiang Gao ◽  
Haihang Ye ◽  
Shulin Wan ◽  
Meghan Pierce ◽  
...  
Keyword(s):  
Colorimetric Assay ◽  
Detection Sensitivity ◽  
Disease Biomarkers ◽  
Enzyme Cascade ◽  
Amplification Strategy ◽  
Cascade Amplification

A non-enzyme cascade amplification strategy for colorimetric assay of disease biomarkers with substantially enhanced detection sensitivity has been developed.

An artificial enzyme cascade amplification strategy for highly sensitive and specific detection of breast cancer-derived exosomes

The Analyst ◽  
2021 ◽  
Author(s):  
Huiying Xu ◽  
Lu Zheng ◽  
Yu Zhou ◽  
Bang-Ce Ye
Keyword(s):  
Early Cancer ◽  
Artificial Enzyme ◽  
Specific Detection ◽  
Heterogeneous Membrane ◽  
Enzyme Cascade ◽  
Early Cancer Diagnosis ◽  
Highly Sensitive ◽  
Noninvasive Biomarkers ◽  
Amplification Strategy ◽  
Cascade Amplification

Tumor-related exosomes, which are heterogeneous membrane-enclosed nanovesicles shed from cancer cells, have been widely recognized as potential noninvasive biomarkers for early cancer diagnosis. Herein, an artificial enzyme cascade amplification strategy...

scholarly journals Quantification of Defensive Proteins in Skin Mucus of Atlantic salmon Using Minimally Invasive Sampling and High-Sensitivity ELISA

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1374
Author(s):  
Haitham Tartor ◽  
Adérito Luis Monjane ◽  
Søren Grove
Keyword(s):  
Atlantic Salmon ◽  
High Sensitivity ◽  
Immunoglobulin M ◽  
Fish Skin ◽  
Skin Mucus ◽  
Enzyme Cascade ◽  
Body Regions ◽  
Amplification Strategy ◽  
Cascade Amplification ◽  
Subtype A

Protocols used to collect fish skin mucus may inadvertently compromise the sampled fish or the resulting sample. Here, we used three methods (wiping, scraping, and absorption) to collect skin mucus from Atlantic salmon and compared their invasiveness on fish skin epithelium. We found that the absorption method was the least invasive. We also compared the abundance of antigen-specific immunoglobulin M subtype A antibodies (IgM-A Ab) and complement component 5 (C5) in mucus samples collected from vaccinated fish by the three methods. An enzyme-cascade-amplification strategy colorimetric immune assay was optimized and used to analyze IgM-A, and ELISA was used to analyze C5. The abundance of antigen-specific IgM-A in skin mucus was comparable between the three methods, but C5 was significantly lower in absorbed mucus in comparison to in the wiped or scraped mucus samples. Absorbed skin mucus samples collected from various body regions of salmon, levels of C5 were comparable, while specific IgM-A amounts varied between the regions. By comparing three mucus-absorbing materials (medical wipe, gauze, and cotton) for their ability to absorb and release IgM-A and C5, medical wipes proved to be ideal for IgM-A analysis, whereas gauze was the best for C5 analysis.

Aptamer-based colorimetric detection of proteins using a branched DNA cascade amplification strategy and unmodified gold nanoparticles

2016 ◽  
Vol 78 ◽  
pp. 200-205 ◽  
Author(s):  
Chia-Chen Chang ◽  
Chen-Yu Chen ◽  
Tsung-Liang Chuang ◽  
Tzu-Heng Wu ◽  
Shu-Chen Wei ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Branched Dna ◽  
Amplification Strategy ◽  
Cascade Amplification

scholarly journals Enzyme-controlled dissolution of MnO2 nanoflakes with enzyme cascade amplification for colorimetric immunoassay

2017 ◽  
Vol 89 ◽  
pp. 645-651 ◽  
Author(s):  
Wenqiang Lai ◽  
Qiaohua Wei ◽  
Mingdi Xu ◽  
Junyang Zhuang ◽  
Dianping Tang
Keyword(s):  
Enzyme Cascade ◽  
Colorimetric Immunoassay ◽  
Cascade Amplification

A cascade signal amplification strategy for sensitive and label-free DNA detection based on Exo III-catalyzed recycling coupled with rolling circle amplification

The Analyst ◽  
2014 ◽  
Vol 139 (11) ◽  
pp. 2884-2889 ◽  
Author(s):  
Xingti Liu ◽  
Qingwang Xue ◽  
Yongshun Ding ◽  
Jing Zhu ◽  
Lei Wang ◽  
...  
Keyword(s):  
Detection Method ◽  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Label Free ◽  
Exonuclease Iii ◽  
Rolling Circle ◽  
Free Dna ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Cascade Amplification

A sensitive and label-free DNA detection method was developed based on cascade amplification combining exonuclease-III recycling with rolling circle amplification.

Sign in / Sign up

Export Citation Format

Share Document