Method to study particle flow bias at a channel bifurcation in a microfluidic device

2017 ◽  
Vol 9 (47) ◽  
pp. 6719-6724
Author(s):  
Melinda A. Lake ◽  
Seth A. Berry ◽  
David J. Hoelzle
Keyword(s):  
Statistical Analysis ◽  
Random Forest ◽  
Microfluidic Device ◽  
Empirical Method ◽  
Particle Flow ◽  
Cell Sorter ◽  
Polystyrene Beads ◽  
Design Variables ◽  
Microfluidic Cell Sorter

We demonstrate an empirical method and a balanced random forest statistical analysis to study the efficacy of microfluidic cell sorter designs with an imbalanced distribution of outcomes. The study uses polystyrene beads as model cells and studies the effects of design variables on the outcome of the beads at a Y-shaped bifurcation.

scholarly journals A Study on the Sustainable Development for Senior Learners

2019 ◽  
Vol 11 (22) ◽  
pp. 6338 ◽  
Author(s):  
Zne-Jung Lee ◽  
Hsiang Huang ◽  
Chou-Yuan Lee ◽  
Yi-Huo Jiang ◽  
Chun-Yen Chang
Keyword(s):  
Decision Making ◽  
Sustainable Development ◽  
Statistical Analysis ◽  
Random Forest ◽  
Decision Rules ◽  
Random Forest Model ◽  
The Sustainable Development ◽  
Forest Model ◽  
Positive Experiences ◽  
The Government

In an aging population with a changing demographic structure, the government aims to ensure that elderly people receive care. In the concept of lifelong learning, education opportunities are available to senior learners, not just children and young people. The sustainable development for senior learners becomes a very important issue because it promotes a variety of learning activities for senior learners. Many universities have started to offer education for senior learners in Taiwan. Positive experiences for senior learners in senior universities can be fostered by ensuring the sustainable development of senior education. In this paper, a study on sustainable development for senior learners is proposed. This study aims to explore potential tools or approaches in evaluating the sustainable development for senior learners for decision making. In this study, two approaches are applied to analyze the sustainable development for senior learners. The first is a statistical analysis, and the second is the random forest model. The methodology of statistical analysis focuses on three aspects such as social assistance, inspiration, and the learning fulfillment for senior learners in senior universities. The random forest model is used to generate decision rules to support decision making. The random forest in this study obtained 22 decision rules. The results suggest that the items in the questionnaire and the decision rules from random forest could provide useful information that allows decision-makers to analyze the sustainable development of senior learners.

scholarly journals Rapid and cheap prototyping of a microfluidic cell sorter

2011 ◽  
Vol 79A (5) ◽  
pp. 361-367 ◽  
Author(s):  
M. Z. Islam ◽  
J. N. McMullin ◽  
Y. Y. Tsui
Keyword(s):  
Cell Sorter ◽  
Microfluidic Cell Sorter

Image-guided microfluidic cell sorter with machine learning

2019 ◽  
Author(s):  
Yi Gu ◽  
Rui Tang ◽  
Alex Ce Zhang ◽  
Yuanyuan Han ◽  
Yu-Hwa Lo
Keyword(s):  
Machine Learning ◽  
Cell Sorter ◽  
Image Guided ◽  
Microfluidic Cell Sorter

scholarly journals Photoresist-based Microfluidic Cell Sorter for Photodynamic Urine Diagnosis

2014 ◽  
Vol 87 ◽  
pp. 62-65
Author(s):  
Yoshikazu Hirai ◽  
Daisuke Takagi ◽  
Satoshi Anai ◽  
Yoshitomo Chihara ◽  
Toshiyuki Tsuchiya ◽  
...  
Keyword(s):  
Cell Sorter ◽  
Microfluidic Cell Sorter

Characterization of Switching Time and Cell Stress in a Gravity-Driven Microfluidic Cell Sorter Based on Hydrodynamic Switching

2010 ◽  
Author(s):  
Michael Grad ◽  
Lubomir Smilenov ◽  
David Brenner ◽  
Daniel Attinger
Keyword(s):  
High Speed ◽  
Bystander Effect ◽  
Fluorescent Protein ◽  
Switching Time ◽  
Blue Dye ◽  
Adhesive Tape ◽  
Buffer Solution ◽  
Cell Sorter ◽  
Microfluidic Cell Sorter

In this work we describe the control and characterization of the switching time and hydrodynamic stress in a microfluidic cell sorter. The device was designed to sort small (<1000) populations of live cells in buffer solution labeled with standard bio-markers such as live dyes or green fluorescent protein (GFP). Sorting occurs through a hydrodynamic switching technique where high-speed solenoid valves control a sheath flow used to steer sorted cells away from the unsorted bulk population. The device is constructed from a reusable hard plastic polymethylmethacrylate (PMMA) chip machined with 127μm × 50μm microchannels and sealed with adhesive tape. Open reservoirs in the chip facilitate pipette access, standard microscope visualization, and a simple disassembly and cleaning procedure. The sorting frequency of this type of device is typically limited by the hydrodynamic switching time. Here, we present a theoretical and numerical analysis of the device switching time. These results show that the sorter switching time t is practically limited by the velocity of the flow and the characteristic length between inlet and outlet channels. We validate this theoretical result with experimental data obtained from flow visualizations, along with experiments conducted to evaluate the repeatability of the hydrodynamic switching scheme and the survival rate of sorted fibroblast cells Manually operated, the sorting frequencies were approximately 10 cells per minute, with switching time constants of approximately 130ms. Current throughput is limited by this switching time to approximately 450 cells per minute. Automation can increase the velocity and reduce the spacing between cells, thereby increasing throughput by at least an order of magnitude. The cell sorter was then tested by manually sorting 100 beads in 7 minutes, and 30 cells in less than 3 minutes, and was successfully used in the framework of a study on the bystander effect occurring during cell irradiation. Experiments with Trypan Blue dye verified that cell viability was maintained during the sorting process.

A Semi-Empirically Thermal Optimization for Heat Sink/TEC Assemblies With Various External Thermal Resistances

2007 ◽  
Author(s):  
M. C. Wu ◽  
T. Y. Wu ◽  
J. T. Horng ◽  
S. F. Chang ◽  
P. L. Chen ◽  
...  
Keyword(s):  
Thermal Resistance ◽  
Thermal Performance ◽  
Network Models ◽  
Empirical Method ◽  
Heat Sinks ◽  
Performance Improvements ◽  
Design Variables ◽  
Fitting Method ◽  
Parametric Studies ◽  
Semi Empirical

An effective semi-empirical method that combines thermal network models and empirical correlations for exploring the thermal performance of heat sinks and HS/TEC assemblies under different external thermal resistances is successfully established. A series of parametric studies, including the effects of external thermal resistance, input current of TEC and pumping heat capacity, on thermal performance improvements of HS/TEC assemblies have been performed. The Response Surface Methodology (RSM) is applied to establish explicit models of the thermal performance of HS/TEC assemblies under various external thermal resistances in terms of the design variables through statistical fitting method. Furthermore, the numerical optimization results for HS/TEC assemblies under different constraints are obtained. With constrained optimal designs of HS/TEC assemblies, the HS/TEC assemblies can provide excellent thermal performance improvements on (1) the reduction of thermal resistance, (2) the enhancement of module heat loads and (3) the improvement of external thermal resistance.

ALA-induced fluorescence detection with photoresist-based microfluidic cell sorter for bladder cancer diagnosis

2015 ◽  
Vol 213 ◽  
pp. 547-557 ◽  
Author(s):  
Yoshikazu Hirai ◽  
Daisuke Takagi ◽  
Satoshi Anai ◽  
Yoshitomo Chihara ◽  
Toshiyuki Tsuchiya ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Fluorescence Detection ◽  
Cancer Diagnosis ◽  
Cell Sorter ◽  
Microfluidic Cell Sorter

scholarly journals Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response

2014 ◽  
Vol 6 (7) ◽  
pp. 714-725 ◽  
Author(s):  
Yongxin Zhao ◽  
Ahmed S. Abdelfattah ◽  
Yufeng Zhao ◽  
Araya Ruangkittisakul ◽  
Klaus Ballanyi ◽  
...  
Keyword(s):  
Directed Evolution ◽  
Calcium Ion ◽  
Cell Sorter ◽  
Fluorescent Response ◽  
Microfluidic Cell Sorter

We describe the use of μFACS to aid the directed evolution of a genetically encoded yellow fluorescent Ca2+indicator.

scholarly journals Microfluidic Cell Sorter (μFCS) for On-chip Capture and Analysis of Single Cells

2012 ◽  
Vol 1 (4) ◽  
pp. 432-436 ◽  
Author(s):  
Jaehoon Chung ◽  
Huilin Shao ◽  
Thomas Reiner ◽  
David Issadore ◽  
Ralph Weissleder ◽  
...  
Keyword(s):  
Single Cells ◽  
Cell Sorter ◽  
On Chip ◽  
Microfluidic Cell Sorter
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