scholarly journals Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response

2014 ◽  
Vol 6 (7) ◽  
pp. 714-725 ◽  
Author(s):  
Yongxin Zhao ◽  
Ahmed S. Abdelfattah ◽  
Yufeng Zhao ◽  
Araya Ruangkittisakul ◽  
Klaus Ballanyi ◽  
...  
Keyword(s):  
Directed Evolution ◽  
Calcium Ion ◽  
Cell Sorter ◽  
Fluorescent Response ◽  
Microfluidic Cell Sorter

We describe the use of μFACS to aid the directed evolution of a genetically encoded yellow fluorescent Ca2+indicator.

A single-phase flow microfluidic cell sorter for multiparameter screening to assist the directed evolution of Ca2+ sensors

Lab on a Chip ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 3880-3887 ◽  
Author(s):  
Yufeng Zhao ◽  
Wei Zhang ◽  
Yongxin Zhao ◽  
Robert E. Campbell ◽  
D. Jed Harrison
Keyword(s):  
Directed Evolution ◽  
Single Phase ◽  
Fluorescent Protein ◽  
Detection System ◽  
Phase Flow ◽  
Bacterial Cells ◽  
Single Phase Flow ◽  
Cell Sorter ◽  
Point Detection ◽  
Microfluidic Cell Sorter

We introduce a single-phase flow microfluidic cell sorter with a two-point detection system capable of two-parameter screening to assist with directed evolution of a fluorescent protein based Ca2+ sensor expressed in bacterial cells.

scholarly journals A Microfluidic Cell Sorter for Directed Evolution of Fluorescent Proteins Based on Dark-State Conversion and Photobleaching

2011 ◽  
Vol 100 (3) ◽  
pp. 175a ◽  
Author(s):  
Jennifer L. Lubbeck ◽  
Kevin M. Dean ◽  
Lloyd M. Davis ◽  
Amy E. Palmer ◽  
Ralph Jimenez
Keyword(s):  
Directed Evolution ◽  
Fluorescent Proteins ◽  
Dark State ◽  
Cell Sorter ◽  
Microfluidic Cell Sorter

scholarly journals Rapid and cheap prototyping of a microfluidic cell sorter

2011 ◽  
Vol 79A (5) ◽  
pp. 361-367 ◽  
Author(s):  
M. Z. Islam ◽  
J. N. McMullin ◽  
Y. Y. Tsui
Keyword(s):  
Cell Sorter ◽  
Microfluidic Cell Sorter

Method to study particle flow bias at a channel bifurcation in a microfluidic device

2017 ◽  
Vol 9 (47) ◽  
pp. 6719-6724
Author(s):  
Melinda A. Lake ◽  
Seth A. Berry ◽  
David J. Hoelzle
Keyword(s):  
Statistical Analysis ◽  
Random Forest ◽  
Microfluidic Device ◽  
Empirical Method ◽  
Particle Flow ◽  
Cell Sorter ◽  
Polystyrene Beads ◽  
Design Variables ◽  
Microfluidic Cell Sorter

We demonstrate an empirical method and a balanced random forest statistical analysis to study the efficacy of microfluidic cell sorter designs with an imbalanced distribution of outcomes. The study uses polystyrene beads as model cells and studies the effects of design variables on the outcome of the beads at a Y-shaped bifurcation.

Image-guided microfluidic cell sorter with machine learning

2019 ◽  
Author(s):  
Yi Gu ◽  
Rui Tang ◽  
Alex Ce Zhang ◽  
Yuanyuan Han ◽  
Yu-Hwa Lo
Keyword(s):  
Machine Learning ◽  
Cell Sorter ◽  
Image Guided ◽  
Microfluidic Cell Sorter

scholarly journals Photoresist-based Microfluidic Cell Sorter for Photodynamic Urine Diagnosis

2014 ◽  
Vol 87 ◽  
pp. 62-65
Author(s):  
Yoshikazu Hirai ◽  
Daisuke Takagi ◽  
Satoshi Anai ◽  
Yoshitomo Chihara ◽  
Toshiyuki Tsuchiya ◽  
...  
Keyword(s):  
Cell Sorter ◽  
Microfluidic Cell Sorter

Characterization of Switching Time and Cell Stress in a Gravity-Driven Microfluidic Cell Sorter Based on Hydrodynamic Switching

2010 ◽  
Author(s):  
Michael Grad ◽  
Lubomir Smilenov ◽  
David Brenner ◽  
Daniel Attinger
Keyword(s):  
High Speed ◽  
Bystander Effect ◽  
Fluorescent Protein ◽  
Switching Time ◽  
Blue Dye ◽  
Adhesive Tape ◽  
Buffer Solution ◽  
Cell Sorter ◽  
Microfluidic Cell Sorter

In this work we describe the control and characterization of the switching time and hydrodynamic stress in a microfluidic cell sorter. The device was designed to sort small (<1000) populations of live cells in buffer solution labeled with standard bio-markers such as live dyes or green fluorescent protein (GFP). Sorting occurs through a hydrodynamic switching technique where high-speed solenoid valves control a sheath flow used to steer sorted cells away from the unsorted bulk population. The device is constructed from a reusable hard plastic polymethylmethacrylate (PMMA) chip machined with 127μm × 50μm microchannels and sealed with adhesive tape. Open reservoirs in the chip facilitate pipette access, standard microscope visualization, and a simple disassembly and cleaning procedure. The sorting frequency of this type of device is typically limited by the hydrodynamic switching time. Here, we present a theoretical and numerical analysis of the device switching time. These results show that the sorter switching time t is practically limited by the velocity of the flow and the characteristic length between inlet and outlet channels. We validate this theoretical result with experimental data obtained from flow visualizations, along with experiments conducted to evaluate the repeatability of the hydrodynamic switching scheme and the survival rate of sorted fibroblast cells Manually operated, the sorting frequencies were approximately 10 cells per minute, with switching time constants of approximately 130ms. Current throughput is limited by this switching time to approximately 450 cells per minute. Automation can increase the velocity and reduce the spacing between cells, thereby increasing throughput by at least an order of magnitude. The cell sorter was then tested by manually sorting 100 beads in 7 minutes, and 30 cells in less than 3 minutes, and was successfully used in the framework of a study on the bystander effect occurring during cell irradiation. Experiments with Trypan Blue dye verified that cell viability was maintained during the sorting process.

ALA-induced fluorescence detection with photoresist-based microfluidic cell sorter for bladder cancer diagnosis

2015 ◽  
Vol 213 ◽  
pp. 547-557 ◽  
Author(s):  
Yoshikazu Hirai ◽  
Daisuke Takagi ◽  
Satoshi Anai ◽  
Yoshitomo Chihara ◽  
Toshiyuki Tsuchiya ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Fluorescence Detection ◽  
Cancer Diagnosis ◽  
Cell Sorter ◽  
Microfluidic Cell Sorter

scholarly journals Microfluidic Cell Sorter (μFCS) for On-chip Capture and Analysis of Single Cells

2012 ◽  
Vol 1 (4) ◽  
pp. 432-436 ◽  
Author(s):  
Jaehoon Chung ◽  
Huilin Shao ◽  
Thomas Reiner ◽  
David Issadore ◽  
Ralph Weissleder ◽  
...  
Keyword(s):  
Single Cells ◽  
Cell Sorter ◽  
On Chip ◽  
Microfluidic Cell Sorter

scholarly journals Cell Sorting: Microfluidic Cell Sorter (μFCS) for On-chip Capture and Analysis of Single Cells (Adv. Healthcare Mater. 4/2012)

2012 ◽  
Vol 1 (4) ◽  
pp. 365-365
Author(s):  
Jaehoon Chung ◽  
Huilin Shao ◽  
Thomas Reiner ◽  
David Issadore ◽  
Ralph Weissleder ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Single Cells ◽  
Cell Sorter ◽  
On Chip ◽  
Microfluidic Cell Sorter
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